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Literature summary for 3.1.1.61 extracted from
- An insight into the interaction mode between CheB and chemoreceptor from two crystal structures of CheB methylesterase catalytic domain (2011), Biochem. Biophys. Res. Commun., 411, 69-75.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Thermotoga maritima |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| CheB methylesterase domain, X-ray diffraction structure determination and analysis at 2.2 A resolution | Thermotoga maritima |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CheB + H2O | Thermotoga maritima | substrate Cheb is a chemoreceptor | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Thermotoga maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CheB + H2O | substrate Cheb is a chemoreceptor | Thermotoga maritima | ? | - |
? | |
| additional information | surfaces with differing electrostatic properties may reflect CheB regions that mediate protein-protein interaction, computational docking, overview | Thermotoga maritima | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | the Thermotoga maritima CheB methylesterase domain has identical topology of a modified doubly-wound alpha/beta fold, but the CheB demethylation consensus sites of the chemoreceptors, the CheB substrate, are not uniquely conserved | Thermotoga maritima |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CheB methylesterase | - |
Thermotoga maritima |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | interaction mode between CheB and chemoreceptors, overview | Thermotoga maritima |
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