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Literature summary for 3.1.1.67 extracted from
- Nonoxidative ethanol metabolism in rabbit myocardium: purification to homogeneity of fatty acyl ethyl ester synthase (1984), Biochemistry, 23, 4075-4081.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-butanol | at concentrations greater than 5times Km | Oryctolagus cuniculus |  |
| 1-propanol | at concentrations greater than 5times Km | Oryctolagus cuniculus | |
| albumin | - |
Oryctolagus cuniculus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
oleate | pH 7.2, 37°C | Oryctolagus cuniculus | |
| 0.2 | - |
palmitate | pH 7.2, 37°C | Oryctolagus cuniculus | |
| 0.2 | - |
linoleate | pH 7.2, 37°C | Oryctolagus cuniculus | |
| 0.2 | - |
stearate | pH 7.2, 37°C | Oryctolagus cuniculus | |
| 0.2 | - |
arachidonate | pH 7.2, 37°C, presence of ethanol | Oryctolagus cuniculus | |
| 70 | - |
1-butanol | pH 7.2, 37°C, cosubstrate oleate | Oryctolagus cuniculus | |
| 530 | - |
1-propanol | pH 7.2, 37°C, cosubstrate oleate | Oryctolagus cuniculus | |
| 1100 | - |
ethanol | pH 7.2, 37°C, cosubstrate oleate | Oryctolagus cuniculus | |
| 1300 | - |
methanol | pH 7.2, 37°C, cosubstrate oleate, value above | Oryctolagus cuniculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Oryctolagus cuniculus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
2 * 26000, SDS-PAGE | Oryctolagus cuniculus |
| 50000 | - |
gel filtration under nondenaturing conditions | Oryctolagus cuniculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| fatty acid + ethanol | Oryctolagus cuniculus | catalyzes fatty acyl ethyl ester synthesis independently of coenzyme A and ATP | fatty acyl ethyl ester + H2O | - |
? | |
| fatty acid + ethanol | Oryctolagus cuniculus | production of a nonoxidative metabolite of ethanol that accumulates in vivo with potentially toxic effects | fatty acyl ethyl ester + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 5400fold | Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | ventricular myocardium | Oryctolagus cuniculus | - |
| myocardium | ventricular | Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Oryctolagus cuniculus |
| 0.0112 | - |
pH 7.2, 37°C | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arachidonate + ethanol | 18.3% of the activity with linoleate | Oryctolagus cuniculus | ethyl arachidonate + H2O | - |
? | |
| fatty acid + ethanol | enzyme specificity | Oryctolagus cuniculus | fatty acyl ethyl ester + H2O | - |
? | |
| fatty acid + ethanol | catalyzes fatty acyl ethyl ester synthesis independently of coenzyme A and ATP | Oryctolagus cuniculus | fatty acyl ethyl ester + H2O | - |
? | |
| fatty acid + ethanol | production of a nonoxidative metabolite of ethanol that accumulates in vivo with potentially toxic effects | Oryctolagus cuniculus | fatty acyl ethyl ester + H2O | - |
? | |
| linoleate + ethanol | highest activity | Oryctolagus cuniculus | ethyl linoleate + H2O | - |
? | |
| additional information | no hydrolysis of cholesteryl oleate | Oryctolagus cuniculus | ? | - |
? | |
| oleate + 1-butanol | 63.2% of the activity with 1-propanol | Oryctolagus cuniculus | 1-butyl oleate + H2O | - |
? | |
| oleate + 1-propanol | highest activity with 1-propanol | Oryctolagus cuniculus | 1-propyl oleate + H2O | - |
? | |
| oleate + ethanol | 79.1% of the activity with linoleate, 81.6% of the activity with 1-propanol | Oryctolagus cuniculus | ethyl oleate + H2O | - |
? | |
| oleate + methanol | 74.7% of the activity with 1-propanol | Oryctolagus cuniculus | methyl oleate + H2O | - |
? | |
| palmitate + ethanol | 7.3% of the activity with linoleate | Oryctolagus cuniculus | ethyl palmitate + H2O | - |
? | |
| stearate + ethanol | 1.6% of the activity with linoleate | Oryctolagus cuniculus | ethyl stearate + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 26000, SDS-PAGE | Oryctolagus cuniculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Oryctolagus cuniculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Oryctolagus cuniculus |
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Release 2023.1 (January 2023)
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