Literature summary for 3.1.1.72 extracted from

Blum, D.L.; Li, X.L.; Chen, H.; Ljungdahl, L.G.
Characterization of an acetyl xylan esterase from the anaerobic fungus Orpinomyces sp. strain PC-2 (1999), Appl. Environ. Microbiol., 65, 3990-3995.

Search for more literature for 3.1.1.72

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination and analysis, expression in Escherichia coli cytosol and periplasm as 2 active forms due to cleavage at 2 different sites within the signal peptide	Orpinomyces sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.9	-	4-nitrophenyl acetate	pH 6.7, 37°C	Orpinomyces sp.

Organism

Organism	UniProt	Comment	Textmining
Orpinomyces sp.	O59869	gene axeA	-

Purification (Commentary)

Purification (Comment)	Organism
2 recombinant enzyme forms from Escherichia coli, over 30fold	Orpinomyces sp.

Reaction

Reaction	Comment	Organism	Reaction ID
acetylxylan + 3 H2O = 3 acetic acid + xylan	catalyses the hydrolysis of acetyl groups from polymeric xylan, acetylated xylose, acetylated glucose, alpha-naphthyl acetate, p-nitrophenyl acetate but not from triacetylglycerol. Does not act on acetylated mannan or pectin.	Orpinomyces sp.	a

Source Tissue

Source Tissue	Comment	Organism	Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.08	-	purified recombinant enzyme, tri-O-acetyl-D-galactal	Orpinomyces sp.
0.2	-	purified recombinant enzyme, xylose tetraacetate	Orpinomyces sp.
0.31	-	purified recombinant enzyme, glucose pentaacetate	Orpinomyces sp.
0.49	-	purified recombinant enzyme, 4-nitrophenyl acetate	Orpinomyces sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl acetate + H2O	-	Orpinomyces sp.	4-nitrophenol + acetate	-	?
acetylated xylan + H2O	the enzyme acts synergistically with xylanase to degrade acetylated xylan	Orpinomyces sp.	xylan + acetate	-	?
glucose pentaacetate + H2O	-	Orpinomyces sp.	glucose + acetate	-	?
tri-O-acetyl-D-galactal + H2O	-	Orpinomyces sp.	D-galactal + acetate	-	?
xylose tetraacetate + H2O	-	Orpinomyces sp.	xylose + acetate	-	?

Subunits

Subunits	Comment	Organism
?	x * 39000-40000, recombinant enzyme, SDS-PAGE	Orpinomyces sp.

Synonyms

Synonyms	Comment	Organism
acetyl xylan esterase	-	Orpinomyces sp.
AxeA	-	Orpinomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	-	Orpinomyces sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	-	Orpinomyces sp.

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Release 2023.1 (January 2023)