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Literature summary for 3.1.1.73 extracted from
- Purification and properties of a feruloyl/p-coumaroyl esterase from the fungus Penicillium pinophilum (1992), Enzyme Microb. Technol., 14, 875-884.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 1 mM markedly increases activity, 5 mM slightly inhibitory | Talaromyces pinophilus |  |
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | selective modification of xylans, degradation may be commercially important | Talaromyces pinophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Talaromyces pinophilus | |
| ethanol | slightly inhibitory at a concentration of 10% | Talaromyces pinophilus | |
| Fe2+ | - |
Aspergillus awamori | |
| Fe2+ | - |
Talaromyces pinophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.08 | - |
p-coumaric acid methyl ester | - |
Talaromyces pinophilus | |
| 0.14 | - |
ferulic acid methyl ester | - |
Talaromyces pinophilus | |
| 1.86 | - |
methyl ferulate | - |
Streptomyces olivochromogenes | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Streptomyces olivochromogenes | - |
- |
| extracellular | - |
Aspergillus awamori | - |
- |
| extracellular | - |
Talaromyces pinophilus | - |
- |
| extracellular | - |
Neocallimastix sp. | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ag2+ | stimulates activity | Talaromyces pinophilus | |
| Ca2+ | stimulates activity | Talaromyces pinophilus | |
| Mg2+ | stimulates activity | Talaromyces pinophilus | |
| Zn2+ | stimulates activity | Talaromyces pinophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 11000 | - |
- |
Neocallimastix sp. |
| 29000 | - |
- |
Streptomyces olivochromogenes |
| 57000 | - |
SDS-PAGE | Talaromyces pinophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| feruloyl xylooligosaccharide + H2O | Aspergillus awamori | - |
ferulic acid + xylooligosaccharide | - |
? | |
| feruloyl xylooligosaccharide + H2O | Talaromyces pinophilus | only feruloylated xylooligomers with specific structures are substrates | ferulic acid + xylooligosaccharide | - |
? | |
| feruloyl xylooligosaccharide + H2O | Streptomyces olivochromogenes | esterified xylooligomers generated by xylanase action | ferulic acid + xylooligosaccharide | - |
? | |
| feruloyl xylooligosaccharide + H2O | Aspergillus oryzae | esterified xylooligomers generated by xylanase action | ferulic acid + xylooligosaccharide | - |
? | |
| feruloyl xylooligosaccharide + H2O | Neocallimastix sp. | esterified xylooligomers generated by xylanase action | ferulic acid + xylooligosaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Streptomyces olivochromogenes | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Aspergillus oryzae | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Schizophyllum commune | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Aspergillus awamori | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Talaromyces pinophilus | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Neocallimastix sp. | - |
ferulate + polysaccharide | - |
? | |
| italian rye gras cell walls + H2O | Aspergillus awamori | - |
ferulic acid + p-coumaric acid + ? | - |
? | |
| italian rye gras cell walls + H2O | Talaromyces pinophilus | - |
ferulic acid + p-coumaric acid + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus awamori | - |
- |
- |
| Aspergillus oryzae | - |
- |
- |
| Neocallimastix sp. | - |
rumen fungus, MC-2 | - |
| Schizophyllum commune | - |
- |
- |
| Streptomyces olivochromogenes | - |
- |
- |
| Talaromyces pinophilus | - |
CMI 87160 ii | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Talaromyces pinophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 28.4 | - |
ferulic acid methyl ester as substrate | Talaromyces pinophilus |
| 33.7 | - |
p-coumaric acid methyl ester | Talaromyces pinophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-naphthyl acetate + H2O | - |
Aspergillus awamori | acetic acid + 1-naphthol | - |
? | |
| 1-naphthyl acetate + H2O | - |
Talaromyces pinophilus | acetic acid + 1-naphthol | - |
? | |
| acetylxylan + H2O | - |
Aspergillus awamori | acetic acid + ? | - |
? | |
| acetylxylan + H2O | - |
Talaromyces pinophilus | acetic acid + ? | - |
? | |
| de-starched wheat bran + H2O | - |
Streptomyces olivochromogenes | ferulic acid + ? | - |
? | |
| de-starched wheat bran + H2O | - |
Schizophyllum commune | ferulic acid + ? | - |
? | |
| de-starched wheat bran + H2O | - |
Talaromyces pinophilus | ferulic acid + ? | - |
? | |
| ferulic acid methyl ester + H2O | - |
Streptomyces olivochromogenes | ferulic acid + methanol | - |
? | |
| ferulic acid methyl ester + H2O | - |
Aspergillus awamori | ferulic acid + methanol | - |
? | |
| ferulic acid methyl ester + H2O | - |
Talaromyces pinophilus | ferulic acid + methanol | - |
? | |
| ferulic acid methyl ester + H2O | - |
Neocallimastix sp. | ferulic acid + methanol | - |
? | |
| feruloyl xylooligosaccharide + H2O | - |
Aspergillus awamori | ferulic acid + xylooligosaccharide | - |
? | |
| feruloyl xylooligosaccharide + H2O | only feruloylated xylooligomers with specific structures are substrates | Talaromyces pinophilus | ferulic acid + xylooligosaccharide | - |
? | |
| feruloyl xylooligosaccharide + H2O | esterified xylooligomers generated by xylanase action | Streptomyces olivochromogenes | ferulic acid + xylooligosaccharide | - |
? | |
| feruloyl xylooligosaccharide + H2O | esterified xylooligomers generated by xylanase action | Aspergillus oryzae | ferulic acid + xylooligosaccharide | - |
? | |
| feruloyl xylooligosaccharide + H2O | esterified xylooligomers generated by xylanase action | Neocallimastix sp. | ferulic acid + xylooligosaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Streptomyces olivochromogenes | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Aspergillus oryzae | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Schizophyllum commune | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Aspergillus awamori | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Talaromyces pinophilus | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Neocallimastix sp. | ferulate + polysaccharide | - |
? | |
| italian rye gras cell walls + H2O | - |
Aspergillus awamori | ferulic acid + p-coumaric acid + ? | - |
? | |
| italian rye gras cell walls + H2O | - |
Talaromyces pinophilus | ferulic acid + p-coumaric acid + ? | - |
? | |
| additional information | - |
Streptomyces olivochromogenes | ? | - |
? | |
| additional information | - |
Aspergillus awamori | ? | - |
? | |
| additional information | - |
Neocallimastix sp. | ? | - |
? | |
| additional information | no capacity to release ferulic and p-coumaric acid from grass cell wall preparations in absence of other xylanolytic enzymes, methyl umbelliferyltrimethyl ammonium cinnamate is no substrate | Talaromyces pinophilus | ? | - |
? | |
| O-5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O | FAXX | Aspergillus awamori | ferulic acid + alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose | - |
? | |
| O-5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O | FAXX | Neocallimastix sp. | ferulic acid + alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose | - |
? | |
| O-5-O-[(E)-p-coumaroyl]-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O | PAXX | Neocallimastix sp. | p-coumaric acid + alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose | - |
? | |
| p-coumaric acid methyl ester + H2O | - |
Streptomyces olivochromogenes | p-coumaric acid + methanol | - |
? | |
| p-coumaric acid methyl ester + H2O | - |
Aspergillus awamori | p-coumaric acid + methanol | - |
? | |
| p-coumaric acid methyl ester + H2O | - |
Talaromyces pinophilus | p-coumaric acid + methanol | - |
? | |
| p-nitrophenyl acetate + H2O | - |
Aspergillus awamori | p-nitrophenol + acetate | - |
? | |
| p-nitrophenyl acetate + H2O | - |
Talaromyces pinophilus | p-nitrophenol + acetate | - |
? | |
| xylan polysaccharide + H2O | - |
Aspergillus oryzae | ferulic acid + p-coumaric acid | - |
? | |
| xylan polysaccharide + H2O | - |
Aspergillus awamori | ferulic acid + p-coumaric acid | - |
? | |
| xylan polysaccharide + H2O | water-soluble wheat straw xylan, enzyme is extremely active on esterified xylan polysaccharide | Talaromyces pinophilus | ferulic acid + p-coumaric acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Aspergillus awamori |
| monomer | - |
Neocallimastix sp. |
| monomer | 1 * 29000, SDS-PAGE | Streptomyces olivochromogenes |
| monomer | 1 * 57000, SDS-PAGE | Talaromyces pinophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
methyl ester of ferulic acid or methyl ester of p-coumaric acid as substrate | Talaromyces pinophilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 70 | - |
Talaromyces pinophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 55 | very stable in this range for up to 8h, at 55°C for 24 h and at 60°C for 2 h still retains 20% original activity, inactive at 60°C for 4 h | Talaromyces pinophilus |
| 37 | 60 | - |
Talaromyces pinophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Talaromyces pinophilus |
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