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Literature summary for 3.1.1.73 extracted from
- Identification of a type-D feruloyl esterase from Neurospora crassa (2004), Appl. Microbiol. Biotechnol., 63, 567-570.
Application
| Application | Comment | Organism |
|---|---|---|
| nutrition | broad range of application in the agri-food industries | Neurospora crassa |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris GS115 (his4) | Neurospora crassa |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Neurospora crassa | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28598 | - |
x * 28598, calculated from the deduced amino acid sequence | Neurospora crassa |
| 32000 | - |
x * 32000, SDS-PAGE | Neurospora crassa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| feruloyl polysaccharide + H2O | Neurospora crassa | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | ferulic acid + p-coumaric acid + ? | - |
? |  |
| spent grain + H2O | Neurospora crassa | incubation together with xylanase | ferulic acid + p-coumaric acid + ? | low percentage of p-coumaric acid | ? | |
| wheat bran + H2O | Neurospora crassa | incubation together with xylanase | ferulic acid + p-coumaric acid + ? | monomeric and dehydrodimers of ferulic acid | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | Q9HGR3 | type D sub-class of the enzyme | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | possible glycosylation hypothesized from the mass difference between the theoretical mass and the SDS-PAGE results | Neurospora crassa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.3 | - |
methyl caffeate as substrate, pH 6.0, 37°C | Neurospora crassa |
| 0.3 | - |
methyl p-coumarate as substrate, pH 6.0, 37°C | Neurospora crassa |
| 0.56 | - |
methyl sinapinate as substrate, pH 6.0, 37°C | Neurospora crassa |
| 0.57 | - |
methyl ferulate as substrate, pH 6.0, 37°C | Neurospora crassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| feruloyl polysaccharide + H2O | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | Neurospora crassa | ferulic acid + p-coumaric acid + ? | - |
? | |
| methyl caffeate + H2O | - |
Neurospora crassa | caffeic acid + methanol | - |
? | |
| methyl ferulate + H2O | - |
Neurospora crassa | ferulic acid + methanol | - |
? | |
| methyl p-coumarate + H2O | - |
Neurospora crassa | p-coumaric acid + methanol | - |
? | |
| methyl sinapinate + H2O | - |
Neurospora crassa | sinapic acid + methanol | - |
? | |
| spent grain + H2O | incubation together with xylanase | Neurospora crassa | ferulic acid + p-coumaric acid + ? | low percentage of p-coumaric acid | ? | |
| wheat bran + H2O | incubation together with xylanase | Neurospora crassa | ferulic acid + p-coumaric acid + ? | monomeric and dehydrodimers of ferulic acid | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32000, SDS-PAGE | Neurospora crassa |
| ? | x * 28598, calculated from the deduced amino acid sequence | Neurospora crassa |
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