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Literature summary for 3.1.1.73 extracted from
- Functional classification of the microbial feruloyl esterases (2004), Appl. Microbiol. Biotechnol., 63, 647-652.
Application
| Application | Comment | Organism |
|---|---|---|
| nutrition | broad range of application in the agri-food industries, production of ferulic as food preservative and as potential precursor for the production of flavor and photo-active agents | Neurospora crassa |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris | Neurospora crassa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| caffeic acid methyl ester | - |
Neurospora crassa |  |
| methyl-3,4-dimethoxy cinnamate | product inhibition | Neurospora crassa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
methyl caffeate | pH 6.0, 37°C | Neurospora crassa | |
| 0.021 | - |
methyl p-coumarate | pH 6.0, 37°C | Neurospora crassa | |
| 0.25 | - |
methyl ferulate | pH 6.0, 37°C | Neurospora crassa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Neurospora crassa | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29286 | - |
x * 29286, calculated from the deduced amino acid sequence, x * 35000, SDS-PAGE, x * 35040, average mass from mass spertrometry | Neurospora crassa |
| 35000 | - |
x * 29286, calculated from the deduced amino acid sequence, x * 35000, SDS-PAGE, x * 35040, average mass from mass spertrometry | Neurospora crassa |
| 35040 | - |
x * 29286, calculated from the deduced amino acid sequence, x * 35000, SDS-PAGE, x * 35040, average mass from mass spertrometry | Neurospora crassa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| feruloyl polysaccharide + H2O | Neurospora crassa | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | ferulic acid + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | Q9HGR3 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 32 mannose residues can be calculated from the difference of the theoretical mass to the experimentally estimated mass | Neurospora crassa |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Neurospora crassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| feruloyl polysaccharide + H2O | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | Neurospora crassa | ferulic acid + ? | - |
? | |
| methyl 3,4-dihydroxycinnamate + H2O | i.e. methyl caffeate | Neurospora crassa | 3,4-dihydroxycinnamic acid + methanol | - |
? | |
| methyl ferulate + H2O | - |
Neurospora crassa | ferulic acid + methanol | - |
? | |
| methyl p-coumarate + H2O | - |
Neurospora crassa | p-coumaric acid + methanol | - |
? | |
| additional information | no activity with methyl sinapinate | Neurospora crassa | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 29286, calculated from the deduced amino acid sequence, x * 35000, SDS-PAGE, x * 35040, average mass from mass spertrometry | Neurospora crassa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cinnamic acid hydrolases | - |
Neurospora crassa |
| Cinnamoyl esterase | - |
Neurospora crassa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Neurospora crassa |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 80 | 25% of activity at 20°C, 35% of activity at 80°C | Neurospora crassa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
unstable above 55°C | Neurospora crassa |
| 60 | - |
80% loss of activity within 10 min, residual activity stable for at least 50 min | Neurospora crassa |
| 70 | 80 | 24% of activity when used for the standard assay with methyl ferulate as substrate | Neurospora crassa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.41 | - |
methyl p-coumarate | pH 6.0, 37°C | Neurospora crassa | |
| 4.8 | - |
methyl caffeate | pH 6.0, 37°C | Neurospora crassa | |
| 5.24 | - |
methyl ferulate | pH 6.0, 37°C | Neurospora crassa | |
| 12.19 | - |
methyl p-coumarate | pH 6.0, 37°C | Neurospora crassa | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Neurospora crassa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | stable | Neurospora crassa |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.5 | - |
caffeic acid methyl ester | pH 6.0 | Neurospora crassa | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Neurospora crassa | calculated from the deduced amino acid sequence | - |
8.26 |
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