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Literature summary for 3.1.1.73 extracted from
- Production and characterization of a type B feruloyl esterase from Fusarium proliferatum NRRL 26517 (2006), Enzyme Microb. Technol., 38, 478-485.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.146 | - |
methyl ferulate | pH 7, 40°C | Fusarium proliferatum |  |
| 0.196 | - |
methyl caffeate | pH 7, 40°C | Fusarium proliferatum | |
| 0.263 | - |
methyl p-coumarate | pH 7, 40°C | Fusarium proliferatum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Fusarium proliferatum | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
x * 31000, SDS-PAGE | Fusarium proliferatum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| corn bran + H2O | Fusarium proliferatum | crude enzyme preparation from the strain supplemented with a commercial xylanase can release about 32% of the alkaline-extractable ferulic acid from corn bran | ferulic acid + ? | - |
? | |
| corn bran + H2O | Fusarium proliferatum NRRL 26517 | crude enzyme preparation from the strain supplemented with a commercial xylanase can release about 32% of the alkaline-extractable ferulic acid from corn bran | ferulic acid + ? | - |
? | |
| feruloyl polysaccharide + H2O | Fusarium proliferatum | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | ferulic acid + ? | - |
? | |
| feruloyl polysaccharide + H2O | Fusarium proliferatum NRRL 26517 | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | ferulic acid + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fusarium proliferatum | - |
feruloyl esterase type B, inorganic nitrogen salt and an esterified ferulic acid in the culture medium required for enzyme production, complex organic nitrogen sources suppress enzyme production | - |
| Fusarium proliferatum NRRL 26517 | - |
feruloyl esterase type B, inorganic nitrogen salt and an esterified ferulic acid in the culture medium required for enzyme production, complex organic nitrogen sources suppress enzyme production | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Fusarium proliferatum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.89 | - |
purified enzyme, pH 7, 40°C | Fusarium proliferatum |
| 3.8 | - |
methyl ferulate as substrate, pH 7, 40°C | Fusarium proliferatum |
| 9.6 | - |
methyl p-coumarate as substrate, pH 7, 40°C | Fusarium proliferatum |
| 13.1 | - |
methyl caffeate as substrate, pH 7, 40°C | Fusarium proliferatum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| corn bran + H2O | crude enzyme preparation from the strain supplemented with a commercial xylanase can release about 32% of the alkaline-extractable ferulic acid from corn bran | Fusarium proliferatum | ferulic acid + ? | - |
? | |
| corn bran + H2O | crude enzyme preparation from the strain supplemented with a commercial xylanase can release about 32% of the alkaline-extractable ferulic acid from corn bran | Fusarium proliferatum NRRL 26517 | ferulic acid + ? | - |
? | |
| feruloyl polysaccharide + H2O | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | Fusarium proliferatum | ferulic acid + ? | - |
? | |
| feruloyl polysaccharide + H2O | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | Fusarium proliferatum NRRL 26517 | ferulic acid + ? | - |
? | |
| methyl caffeate + H2O | - |
Fusarium proliferatum | caffeic acid + methanol | - |
? | |
| methyl caffeate + H2O | - |
Fusarium proliferatum NRRL 26517 | caffeic acid + methanol | - |
? | |
| methyl ferulate + H2O | - |
Fusarium proliferatum | ferulic acid + methanol | - |
? | |
| methyl ferulate + H2O | - |
Fusarium proliferatum NRRL 26517 | ferulic acid + methanol | - |
? | |
| methyl p-coumarate + H2O | - |
Fusarium proliferatum | p-coumaric acid + methanol | - |
? | |
| additional information | no activity with methyl sinapinate | Fusarium proliferatum | ? | - |
? | |
| additional information | no activity with methyl sinapinate | Fusarium proliferatum NRRL 26517 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 31000, SDS-PAGE | Fusarium proliferatum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Fusarium proliferatum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
unstable above 50°C | Fusarium proliferatum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | - |
Fusarium proliferatum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
enzyme retains over 80% of its activity at pH 5.0 after storage at for 12 h at 4°C | Fusarium proliferatum |
| 6 | 8 | stable at pHs between 6 and 8 | Fusarium proliferatum |
| 9 | - |
enzyme retains over 80% of its activity at pH 9.0 after storage at for 12 h at 4°C | Fusarium proliferatum |
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