Cloned (Comment) | Organism |
---|---|
Y80 and W260 mutants expressed in Pichia pastoris | Aspergillus niger |
Crystallization (Comment) | Organism |
---|---|
S133A mutant in complex with O-(5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl)-(1,3)-O-beta-D-xylopyranosyl-(1,4)-D-xylopyranose, hanging drop vapor diffusion method | Aspergillus niger |
Protein Variants | Comment | Organism |
---|---|---|
S133A | inactive | Aspergillus niger |
W260S | significantly reduced turnover, increase in alpha-helix content, broadening of substrate specificity allowing the hydrolysis of methyl caffeate | Aspergillus niger |
W260V | significantly reduced turnover, increase in alpha-helix content, broadening of substrate specificity allowing the hydrolysis of methyl caffeate | Aspergillus niger |
Y80S | significantly reduced turnover, increase in alpha-helix content, broadening of substrate specificity allowing the hydrolysis of methyl caffeate | Aspergillus niger |
Y80V | significantly reduced turnover, increase in alpha-helix content, broadening of substrate specificity allowing the hydrolysis of methyl caffeate | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
methyl sinapinate | pH 6.0, 37°C, native enzyme | Aspergillus niger | |
0.35 | - |
methyl sinapinate | pH 6.0, 37°C, W260S mutant enzyme | Aspergillus niger | |
0.76 | - |
methyl sinapinate | pH 6.0, 37°C, W260V mutant enzyme | Aspergillus niger | |
0.78 | - |
methyl ferulate | pH 6.0, 37°C, native enzyme | Aspergillus niger | |
0.84 | - |
methyl sinapinate | pH 6.0, 37°C, Y80S mutant enzyme | Aspergillus niger | |
0.88 | - |
methyl ferulate | pH 6.0, 37°C, W260S mutant enzyme | Aspergillus niger | |
1.01 | - |
methyl ferulate | pH 6.0, 37°C, W260V mutant enzyme | Aspergillus niger | |
1.17 | - |
methyl ferulate | pH 6.0, 37°C, Y80V mutant enzyme | Aspergillus niger | |
1.22 | - |
methyl ferulate | pH 6.0, 37°C, Y80S mutant enzyme | Aspergillus niger | |
1.23 | - |
methyl sinapinate | pH 6.0, 37°C, Y80V mutant enzyme | Aspergillus niger | |
2.07 | - |
methyl p-coumarate | pH 6.0, 37°C, Y80S mutant enzyme | Aspergillus niger | |
2.88 | - |
methyl p-coumarate | pH 6.0, 37°C, Y80V mutant enzyme | Aspergillus niger | |
3.02 | - |
methyl caffeate | pH 6.0, 37°C, Y80V mutant enzyme | Aspergillus niger | |
3.26 | - |
methyl p-coumarate | pH 6.0, 37°C, W260S mutant enzyme | Aspergillus niger | |
3.84 | - |
methyl caffeate | pH 6.0, 37°C, Y80S mutant enzyme | Aspergillus niger | |
3.99 | - |
methyl p-coumarate | pH 6.0, 37°C, W260V mutant enzyme | Aspergillus niger | |
4.1 | - |
methyl caffeate | pH 6.0, 37°C, W260S mutant enzyme | Aspergillus niger | |
4.26 | - |
methyl p-coumarate | pH 6.0, 37°C, native enzyme | Aspergillus niger | |
4.72 | - |
methyl caffeate | pH 6.0, 37°C, W260V mutant enzyme | Aspergillus niger |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Aspergillus niger | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
feruloyl polysaccharide + H2O | Aspergillus niger | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | ferulic acid + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
type A enzyme | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
feruloyl polysaccharide + H2O | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | Aspergillus niger | ferulic acid + ? | - |
? | |
methyl caffeate + H2O | no activity of native enzyme, mutant enzymes are capable in hydrolyzing methyl caffeate | Aspergillus niger | caffeic acid + methanol | - |
? | |
methyl ferulate + H2O | - |
Aspergillus niger | ferulic acid + methanol | - |
? | |
methyl p-coumarate + H2O | - |
Aspergillus niger | p-coumaric acid + methanol | - |
? | |
methyl sinapinate + H2O | - |
Aspergillus niger | sinapic acid + methanol | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
methyl caffeate | pH 6.0, 37°C, Y80S mutant enzyme | Aspergillus niger | |
0.02 | - |
methyl caffeate | pH 6.0, 37°C, Y80V mutant enzyme | Aspergillus niger | |
0.031 | 0.51 | methyl sinapinate | pH 6.0, 37°C, Y80V mutant enzyme | Aspergillus niger | |
0.1 | - |
methyl p-coumarate | pH 6.0, 37°C, Y80S mutant enzyme | Aspergillus niger | |
0.26 | - |
methyl caffeate | pH 6.0, 37°C, W260V mutant enzyme | Aspergillus niger | |
0.26 | - |
methyl p-coumarate | pH 6.0, 37°C, Y80V mutant enzyme | Aspergillus niger | |
0.29 | - |
methyl p-coumarate | pH 6.0, 37°C, W260V mutant enzyme | Aspergillus niger | |
0.32 | - |
methyl caffeate | pH 6.0, 37°C, W260S mutant enzyme | Aspergillus niger | |
0.49 | - |
methyl p-coumarate | pH 6.0, 37°C, W260S mutant enzyme | Aspergillus niger | |
0.73 | - |
methyl p-coumarate | pH 6.0, 37°C, native enzyme | Aspergillus niger | |
0.99 | - |
methyl ferulate | pH 6.0, 37°C, W260V mutant enzyme | Aspergillus niger | |
1.56 | - |
methyl ferulate | pH 6.0, 37°C, Y80S mutant enzyme | Aspergillus niger | |
1.6 | - |
methyl ferulate | pH 6.0, 37°C, native enzyme | Aspergillus niger | |
2 | 3.7 | methyl sinapinate | pH 6.0, 37°C, native enzyme | Aspergillus niger | |
2.56 | - |
methyl ferulate | pH 6.0, 37°C, Y80V mutant enzyme | Aspergillus niger | |
3.48 | - |
methyl sinapinate | pH 6.0, 37°C, Y80S mutant enzyme | Aspergillus niger | |
3.5 | - |
methyl ferulate | pH 6.0, 37°C, W260S mutant enzyme | Aspergillus niger | |
7.85 | - |
methyl sinapinate | pH 6.0, 37°C, Y80V mutant enzyme | Aspergillus niger | |
18.33 | - |
methyl ferulate | pH 6.0, 37°C, W260V mutant enzyme | Aspergillus niger | |
20.06 | - |
methyl ferulate | pH 6.0, 37°C, W260S mutant enzyme | Aspergillus niger | |
27.76 | - |
methyl sinapinate | pH 6.0, 37°C, W260S mutant enzyme | Aspergillus niger | |
28.28 | - |
methyl sinapinate | pH 6.0, 37°C, W260V mutant enzyme | Aspergillus niger | |
70.74 | - |
methyl ferulate | pH 6.0, 37°C, native enzyme | Aspergillus niger | |
84.95 | - |
methyl sinapinate | pH 6.0, 37°C, native enzyme | Aspergillus niger |