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Literature summary for 3.1.1.73 extracted from

  • Garcia-Conesa, M.T.; Crepin, V.F.; Goldson, A.J.; Williamson, G.; Cummings, N.J.; Connerton, I.F.; Faulds, C.B.; Kroon, P.A.
    The feruloyl esterase system of Talaromyces stipitatus: production of three discrete feruloyl esterases, including a novel enzyme, TsFaeC, with a broad substrate specificity (2004), J. Biotechnol., 108, 227-241.
    View publication on PubMed
Search for more literature for 3.1.1.73

Cloned(Commentary)

Cloned (Comment) Organism
type C enzyme Talaromyces stipitatus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Talaromyces stipitatus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 x * 35000, type A and B enzymes, SDS-PAGE Talaromyces stipitatus
55340
-
 x * 55340, type C enzyme, calculated from the deduced amino acid sequence, x * 66000, smeared band on SDS-PAGE Talaromyces stipitatus
66000
-
 x * 55340, type C enzyme, calculated from the deduced amino acid sequence, x * 66000, smeared band on SDS-PAGE Talaromyces stipitatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
feruloyl polysaccharide + H2O Talaromyces stipitatus involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components ferulic acid + ?
-
 ?
feruloyl polysaccharide + H2O Talaromyces stipitatus CBS 375.48 involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components ferulic acid + ?
-
 ?
sugar beat pulp + H2O Talaromyces stipitatus
-
 ferulic acid + ?
-
 ?
sugar beat pulp + H2O Talaromyces stipitatus CBS 375.48
-
 ferulic acid + ?
-
 ?
wheat bran + H2O Talaromyces stipitatus
-
 ferulic acid + ?
-
 ?
wheat bran + H2O Talaromyces stipitatus CBS 375.48
-
 ferulic acid + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Talaromyces stipitatus
-
 feruloyl ersterasea type A and B
-
Talaromyces stipitatus Q70Y21 feruloyl ersterasea type C
-
Talaromyces stipitatus CBS 375.48
-
 feruloyl ersterasea type A and B
-
Talaromyces stipitatus CBS 375.48 Q70Y21 feruloyl ersterasea type C
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein difference between theoretical and detected molecular mass indicates glycosylation Talaromyces stipitatus

Purification (Commentary)

Purification (Comment) Organism
type A and B enzyme Talaromyces stipitatus
type C enzyme Talaromyces stipitatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
feruloyl polysaccharide + H2O involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components Talaromyces stipitatus ferulic acid + ?
-
 ?
feruloyl polysaccharide + H2O involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components Talaromyces stipitatus CBS 375.48 ferulic acid + ?
-
 ?
methyl caffeate + H2O
-
 Talaromyces stipitatus caffeic acid + methanol
-
 ?
methyl caffeate + H2O very low activity of type A enzyme Talaromyces stipitatus caffeic acid + methanol
-
 ?
methyl caffeate + H2O very low activity of type A enzyme Talaromyces stipitatus CBS 375.48 caffeic acid + methanol
-
 ?
methyl caffeate + H2O
-
 Talaromyces stipitatus CBS 375.48 caffeic acid + methanol
-
 ?
methyl ferulate + H2O
-
 Talaromyces stipitatus ferulic acid + methanol
-
 ?
methyl ferulate + H2O low activity of type B enzyme Talaromyces stipitatus ferulic acid + methanol
-
 ?
methyl p-coumarate + H2O
-
 Talaromyces stipitatus p-coumaric acid + methanol
-
 ?
methyl p-coumarate + H2O very low activity of type A enzyme Talaromyces stipitatus p-coumaric acid + methanol
-
 ?
methyl sinapinate + H2O
-
 Talaromyces stipitatus sinapic acid + methanol
-
 ?
methyl sinapinate + H2O
-
 Talaromyces stipitatus sinapic acid + methanol no activity of type B enzyme ?
methyl sinapinate + H2O
-
 Talaromyces stipitatus CBS 375.48 sinapic acid + methanol no activity of type B enzyme ?
methyl sinapinate + H2O
-
 Talaromyces stipitatus CBS 375.48 sinapic acid + methanol
-
 ?
O-[5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl]-(1,3)-O-beta-D-xylopyranosyl-(1,4)-D-xylopyranose + H2O high activity of type A enzyme, moderate or low activity of type C and B enzymes Talaromyces stipitatus ferulic acid + ?
-
 ?
O-[5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl]-(1,3)-O-beta-D-xylopyranosyl-(1,4)-D-xylopyranose + H2O low activity of type C enzyme Talaromyces stipitatus ferulic acid + ?
-
 ?
sugar beat pulp + H2O
-
 Talaromyces stipitatus ferulic acid + ?
-
 ?
sugar beat pulp + H2O
-
 Talaromyces stipitatus CBS 375.48 ferulic acid + ?
-
 ?
wheat bran + H2O
-
 Talaromyces stipitatus ferulic acid + ?
-
 ?
wheat bran + H2O
-
 Talaromyces stipitatus CBS 375.48 ferulic acid + ?
-
 ?
[2-O-(trans-feruloyl)-alpha-L-arabinofuranosyl]-(1,5)-L-arabinofuranose + H2O
-
 Talaromyces stipitatus ferulic acid + ?
-
 ?
[2-O-(trans-feruloyl)-alpha-L-arabinofuranosyl]-(1,5)-L-arabinofuranose + H2O no activity of type A enzyme Talaromyces stipitatus ferulic acid + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 35000, type A and B enzymes, SDS-PAGE Talaromyces stipitatus
? x * 55340, type C enzyme, calculated from the deduced amino acid sequence, x * 66000, smeared band on SDS-PAGE Talaromyces stipitatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 type C enzyme Talaromyces stipitatus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70
-
 20% of activity Talaromyces stipitatus
80
-
 4% of activity Talaromyces stipitatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 unstable on incubation above 40°C Talaromyces stipitatus
60
-
 almost complete loss of activity within 10 min Talaromyces stipitatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7 type C enzyme Talaromyces stipitatus

pH Range

pH Minimum pH Maximum Comment Organism
2.6
-
 34% of activity Talaromyces stipitatus
4
-
 50% of activity Talaromyces stipitatus
5.6
-
 95% of activity Talaromyces stipitatus
7.7
-
 88% of activity Talaromyces stipitatus

pI Value

Organism Comment pI Value Maximum pI Value
Talaromyces stipitatus type A enzyme, isoelectric focusing
-
 3.5
Talaromyces stipitatus type C enzyme, isoelectric focusing
-
 4.6
Talaromyces stipitatus type B enzyme, isoelectric focusing
-
 5.3