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Literature summary for 3.1.1.73 extracted from

  • Prates, J.A.; Tarbouriech, N.; Charnock, S.J.; Fontes, C.M.; Ferreira, L.M.; Davies, G.J.
    The structure of the feruloyl esterase module of xylanase 10B from Clostridium thermocellum provides insights into substrate recognition (2001), Structure, 9, 1183-1190.
    View publication on PubMed

Application

Application Comment Organism
medicine potential application of ferulic acid as antioxidant and anti-cancer agent Acetivibrio thermocellus
nutrition ferulic acid is a potential precursor for the synthesis of aroma and flavoring compounds Acetivibrio thermocellus

Cloned(Commentary)

Cloned (Comment) Organism
-
Acetivibrio thermocellus

Crystallization (Commentary)

Crystallization (Comment) Organism
native and mutant protein, hanging drop vapor diffusion method Acetivibrio thermocellus

Protein Variants

Protein Variants Comment Organism
S954A inactive, unable to bind the sugar moiety of the substrate Acetivibrio thermocellus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular located in the cellulosome Acetivibrio thermocellus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
feruloyl polysaccharide + H2O Acetivibrio thermocellus involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components ferulic acid + ?
-
?
additional information Acetivibrio thermocellus hydrolyzes the ester linkage between arabinofuranoside side chains and ferulic acid ?
-
?

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme using His-tag Acetivibrio thermocellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
feruloyl polysaccharide + H2O involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components Acetivibrio thermocellus ferulic acid + ?
-
?
additional information hydrolyzes the ester linkage between arabinofuranoside side chains and ferulic acid Acetivibrio thermocellus ?
-
?

Synonyms

Synonyms Comment Organism
xylanase 10 ferulate esterase domains are components of the multimodular xylanases 10A and 10B Acetivibrio thermocellus