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Literature summary for 3.1.1.73 extracted from

  • Aurilia, V.; Parracino, A.; Saviano, M.; Rossi, M.; DAuria, S.
    The psychrophilic bacterium Pseudoalteromonas haloplanktis TAC125 possesses a gene coding for a cold-adapted feruloyl esterase activity that shares homology with esterase enzymes from gamma-proteobacteria and yeast (2007), Gene, 397, 51-57.
    View publication on PubMed
Search for more literature for 3.1.1.73

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudoalteromonas haloplanktis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000025
-
 methylumbelliferyl acetate 20°C, pH 7.4 Pseudoalteromonas haloplanktis
0.27
-
 4-methylumbelliferyl p-trimethylammonio cinnamate 20°C, pH 7.4 Pseudoalteromonas haloplanktis
0.5
-
 Alpha-naphthyl acetate 20°C, pH 7.4 Pseudoalteromonas haloplanktis
0.5
-
 beta-naphthyl acetate 20°C, pH 7.4 Pseudoalteromonas haloplanktis
0.75
-
 p-nitrophenyl acetate 20°C, pH 7.4 Pseudoalteromonas haloplanktis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stimulates Pseudoalteromonas haloplanktis
Mg2+ stimulates Pseudoalteromonas haloplanktis
Mn2+ stimulates Pseudoalteromonas haloplanktis

Organism

Organism UniProt Comment Textmining
Pseudoalteromonas haloplanktis Q3IL66
-
-
Pseudoalteromonas haloplanktis TAC 125 Q3IL66
-
-

Storage Stability

Storage Stability Organism
4°C, 3 days, stable Pseudoalteromonas haloplanktis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl p-trimethylammonio cinnamate + H2O
-
 Pseudoalteromonas haloplanktis 4-methylumbelliferone + p-trimethylammonio cinnamate
-
 ?
4-methylumbelliferyl p-trimethylammonio cinnamate + H2O
-
 Pseudoalteromonas haloplanktis TAC 125 4-methylumbelliferone + p-trimethylammonio cinnamate
-
 ?
alpha-naphthyl acetate + H2O
-
 Pseudoalteromonas haloplanktis alpha-naphthol + acetate
-
 ?
alpha-naphthyl acetate + H2O
-
 Pseudoalteromonas haloplanktis TAC 125 alpha-naphthol + acetate
-
 ?
beta-naphthyl acetate + H2O
-
 Pseudoalteromonas haloplanktis beta-naphthol + acetate
-
 ?
beta-naphthyl acetate + H2O
-
 Pseudoalteromonas haloplanktis TAC 125 beta-naphthol + acetate
-
 ?
methylumbelliferyl acetate + H2o
-
 Pseudoalteromonas haloplanktis methylumbelliferone + acetate
-
 ?
additional information no activity with p-nitrophenyl esanoate and p-nitrophenyl-dodecanoate Pseudoalteromonas haloplanktis ?
-
 ?
additional information no activity with p-nitrophenyl esanoate and p-nitrophenyl-dodecanoate Pseudoalteromonas haloplanktis TAC 125 ?
-
 ?
p-nitrophenyl acetate + H2O
-
 Pseudoalteromonas haloplanktis p-nitrophenol + acetate
-
 ?
p-nitrophenyl acetate + H2O
-
 Pseudoalteromonas haloplanktis TAC 125 p-nitrophenol + acetate
-
 ?

Subunits

Subunits Comment Organism
? x * 30000., SDS-PAGE Pseudoalteromonas haloplanktis

Synonyms

Synonyms Comment Organism
PSHAa enzyme
-
 Pseudoalteromonas haloplanktis
PSHAa1385
-
 Pseudoalteromonas haloplanktis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
-
 Pseudoalteromonas haloplanktis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 methylumbelliferyl acetate 20°C, pH 7.4 Pseudoalteromonas haloplanktis
1.8
-
 beta-naphthyl acetate 20°C, pH 7.4 Pseudoalteromonas haloplanktis
2
-
 p-nitrophenyl acetate 20°C, pH 7.4 Pseudoalteromonas haloplanktis
2.75
-
 Alpha-naphthyl acetate 20°C, pH 7.4 Pseudoalteromonas haloplanktis
11.9
-
 4-methylumbelliferyl p-trimethylammonio cinnamate 20°C, pH 7.4 Pseudoalteromonas haloplanktis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pseudoalteromonas haloplanktis