Literature summary for 3.1.1.73 extracted from

Puchart, V.; Vrsanska, M.; Mastihubova, M.; Topakas, E.; Vafiadi, C.; Faulds, C.B.; Tenkanen, M.; Christakopoulos, P.; Biely, P.
Substrate and positional specificity of feruloyl esterases for monoferuloylated and monoacetylated 4-nitrophenyl glycosides (2007), J. Biotechnol., 127, 235-243.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.016	-	4-nitrophenyl 5-O-(E)-feruloyl-alpha-L-arabinofuranoside	-	Talaromyces stipitatus
0.028	-	4-nitrophenyl 4-O-(E)-feruloyl-alpha-D-xylopyranoside	-	Talaromyces stipitatus
0.042	-	4-nitrophenyl 2-O-(E)-feruloyl-alpha-L-arabinofuranoside	-	Talaromyces stipitatus
0.053	-	4-nitrophenyl 3-O-(E)-feruloyl-alpha-L-arabinofuranoside	-	Talaromyces stipitatus
0.073	-	4-nitrophenyl 5-O-(E)-feruloyl-alpha-L-arabinofuranoside	-	Aspergillus niger
0.097	-	4-nitrophenyl 2-O-(E)-feruloyl-alpha-D-xylopyranoside	-	Talaromyces stipitatus
0.123	-	4-nitrophenyl 5-O-(E)-feruloyl-alpha-L-arabinofuranoside	-	Fusarium oxysporum
0.24	-	4-nitrophenyl 4-O-(E)-feruloyl-alpha-D-xylopyranoside	-	Aspergillus niger
0.25	-	4-nitrophenyl 3-O-(E)-feruloyl-alpha-L-arabinofuranoside	-	Fusarium oxysporum
0.28	-	4-nitrophenyl 5-O-(E)-feruloyl-alpha-L-arabinofuranoside	-	Fusarium oxysporum
0.54	-	4-nitrophenyl 2-O-(E)-feruloyl-alpha-L-arabinofuranoside	-	Fusarium oxysporum
0.57	-	4-nitrophenyl 3-O-(E)-feruloyl-alpha-D-xylopyranoside	-	Fusarium oxysporum
1.24	-	4-nitrophenyl 5-O-acetyl-alpha-L-arabinofuranoside	-	Aspergillus niger
1.5	2	4-nitrophenyl 2-O-(E)-feruloyl-alpha-D-xylopyranoside	-	Aspergillus niger
1.51	-	4-nitrophenyl 3-O-(E)-feruloyl-alpha-D-xylopyranoside	-	Aspergillus niger
2.26	-	4-nitrophenyl 2-O-(E)-feruloyl-alpha-D-xylopyranoside	-	Fusarium oxysporum
3.82	-	4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside	-	Aspergillus oryzae
6.78	-	4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside	-	Aspergillus oryzae
6.98	-	4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside	-	Fusarium oxysporum
9.44	-	4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside	-	Aspergillus oryzae
16.4	-	4-nitrophenyl 5-O-acetyl-alpha-L-arabinofuranoside	-	Aspergillus oryzae
17	-	4-nitrophenyl 5-O-acetyl-alpha-L-arabinofuranoside	-	Fusarium oxysporum
44.6	-	4-nitrophenyl 2-O-acetyl-alpha-L-arabinofuranoside	-	Aspergillus oryzae

Organism

Organism	UniProt	Comment	Textmining
Aspergillus niger	O42807	-	-
Aspergillus oryzae	-	-	-
Fusarium oxysporum	-	-	-
Talaromyces stipitatus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
4-nitrophenyl 2-O-(E)-feruloyl-alpha-D-xylopyranoside + H2O	-	Fusarium oxysporum	4-nitrophenyl-alpha-D-xylopyranoside + ferulate	-	?
4-nitrophenyl 2-O-(E)-feruloyl-alpha-D-xylopyranoside + H2O	-	Talaromyces stipitatus	4-nitrophenyl-alpha-D-xylopyranoside + trans-ferulate	-	?
4-nitrophenyl 2-O-(E)-feruloyl-alpha-D-xylopyranoside + H2O	-	Aspergillus niger	4-nitrophenyl-alpha-D-xylopyranoside + trans-ferulate	-	?
4-nitrophenyl 2-O-(E)-feruloyl-alpha-L-arabinofuranoside + H2O	-	Fusarium oxysporum	4-nitrophenyl-alpha-L-arabinofuranoside + ferulate	-	?
4-nitrophenyl 2-O-(E)-feruloyl-alpha-L-arabinofuranoside + H2O	-	Talaromyces stipitatus	4-nitrophenyl-alpha-L-arabinofuranoside + trans-ferulate	-	?
4-nitrophenyl 2-O-acetyl-alpha-L-arabinofuranoside + H2O	-	Aspergillus oryzae	4-nitrophenyl-alpha-L-arabinofuranoside + acetate	-	?
4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside + H2O	-	Aspergillus oryzae	4-nitrophenyl-beta-D-xylopyranoside + acetate	-	?
4-nitrophenyl 3-O-(E)-feruloyl-alpha-D-xylopyranoside + H2O	-	Fusarium oxysporum	4-nitrophenyl-alpha-D-xylopyranoside + ferulate	-	?
4-nitrophenyl 3-O-(E)-feruloyl-alpha-D-xylopyranoside + H2O	-	Aspergillus niger	4-nitrophenyl-alpha-D-xylopyranoside + trans-ferulate	-	?
4-nitrophenyl 3-O-(E)-feruloyl-alpha-L-arabinofuranoside + H2O	-	Fusarium oxysporum	4-nitrophenyl-alpha-L-arabinofuranoside + ferulate	-	?
4-nitrophenyl 3-O-(E)-feruloyl-alpha-L-arabinofuranoside + H2O	-	Talaromyces stipitatus	4-nitrophenyl alpha-L-arabinofuranoside + ferulate	-	?
4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside + H2O	-	Aspergillus oryzae	4-nitrophenyl-beta-D-xylopyranoside + acetate	-	?
4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside + H2O	-	Fusarium oxysporum	?	-	?
4-nitrophenyl 4-O-(E)-feruloyl-alpha-D-xylopyranoside + H2O	-	Talaromyces stipitatus	4-nitrophenyl-alpha-D-xylopyranoside + trans-ferulate	-	?
4-nitrophenyl 4-O-(E)-feruloyl-alpha-D-xylopyranoside + H2O	-	Aspergillus niger	4-nitrophenyl-alpha-D-xylopyranoside + trans-ferulate	-	?
4-nitrophenyl 4-O-acetyl-beta-D-xylopyranoside + H2O	-	Aspergillus oryzae	4-nitrophenyl-beta-D-xylopyranoside + acetate	-	?
4-nitrophenyl 4-O-acetyl-beta-D-xylopyranoside + H2O	-	Fusarium oxysporum	4-nitrophenyl-beta-D-xylopyranoside + acetate	-	?
4-nitrophenyl 5-O-(E)-feruloyl-alpha-L-arabinofuranoside + H2O	-	Fusarium oxysporum	4-nitrophenyl-alpha-L-arabinofuranoside + ferulate	-	?
4-nitrophenyl 5-O-(E)-feruloyl-alpha-L-arabinofuranoside + H2O	-	Fusarium oxysporum	4-nitrophenyl-alpha-L-arabinofuranoside + trans-ferulate	-	?
4-nitrophenyl 5-O-(E)-feruloyl-alpha-L-arabinofuranoside + H2O	-	Talaromyces stipitatus	4-nitrophenyl alpha-L-arabinofuranoside + ferulate	-	?
4-nitrophenyl 5-O-(E)-feruloyl-alpha-L-arabinofuranoside + H2O	-	Aspergillus niger	4-nitrophenyl alpha-L-arabinofuranoside + ferulate	-	?
4-nitrophenyl 5-O-acetyl-alpha-L-arabinofuranoside + H2O	-	Aspergillus oryzae	4-nitrophenyl-alpha-L-arabinofuranoside + acetate	-	?
4-nitrophenyl 5-O-acetyl-alpha-L-arabinofuranoside + H2O	-	Fusarium oxysporum	4-nitrophenyl-alpha-L-arabinofuranoside + acetate	-	?
4-nitrophenyl 5-O-acetyl-alpha-L-arabinofuranoside + H2O	the enzyme is specialized for deferuloylation of primary hydroxyl groups, with a very strong preference for hydrolyzing 5-O-feruloyl-alpha-L-arabinofuranoside	Aspergillus niger	4-nitrophenyl-alpha-L-arabinofuranoside + acetate	-	?

Synonyms

Synonyms	Comment	Organism
AnFaeA	-	Aspergillus niger
AoFaeC	-	Aspergillus oryzae
FoFaeI	-	Fusarium oxysporum
FoFaeII	-	Fusarium oxysporum
TsFaeC	-	Talaromyces stipitatus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.03	0.55	4-nitrophenyl 2-O-(E)-feruloyl-alpha-L-arabinofuranoside	-	Fusarium oxysporum
0.15	-	4-nitrophenyl 2-O-acetyl-alpha-L-arabinofuranoside	-	Aspergillus oryzae
0.24	-	4-nitrophenyl 4-O-(E)-feruloyl-alpha-D-xylopyranoside	-	Aspergillus niger
0.7	-	4-nitrophenyl 3-O-(E)-feruloyl-alpha-D-xylopyranoside	-	Aspergillus niger
0.81	-	4-nitrophenyl 3-O-(E)-feruloyl-alpha-L-arabinofuranoside	-	Fusarium oxysporum
0.86	-	4-nitrophenyl 3-O-(E)-feruloyl-alpha-D-xylopyranoside	-	Fusarium oxysporum
0.89	-	4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside	-	Fusarium oxysporum
0.91	-	4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside	-	Aspergillus oryzae
1.17	-	4-nitrophenyl 2-O-(E)-feruloyl-alpha-D-xylopyranoside	-	Aspergillus niger
1.77	-	4-nitrophenyl 5-O-acetyl-alpha-L-arabinofuranoside	-	Aspergillus oryzae
1.9	-	4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside	-	Aspergillus oryzae
2.33	-	4-nitrophenyl 5-O-acetyl-alpha-L-arabinofuranoside	-	Aspergillus niger
2.73	-	4-nitrophenyl 5-O-(E)-feruloyl-alpha-L-arabinofuranoside	-	Fusarium oxysporum
3.23	-	4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside	-	Aspergillus oryzae
3.66	-	4-nitrophenyl 5-O-(E)-feruloyl-alpha-L-arabinofuranoside	-	Fusarium oxysporum
5.2	-	4-nitrophenyl 2-O-(E)-feruloyl-alpha-D-xylopyranoside	-	Fusarium oxysporum
6.37	-	4-nitrophenyl 5-O-acetyl-alpha-L-arabinofuranoside	-	Fusarium oxysporum
7.05	-	4-nitrophenyl 2-O-(E)-feruloyl-alpha-L-arabinofuranoside	-	Fusarium oxysporum
17.9	-	4-nitrophenyl 4-O-(E)-feruloyl-alpha-D-xylopyranoside	-	Talaromyces stipitatus
22.5	-	4-nitrophenyl 5-O-(E)-feruloyl-alpha-L-arabinofuranoside	-	Talaromyces stipitatus
34.2	-	4-nitrophenyl 3-O-(E)-feruloyl-alpha-L-arabinofuranoside	-	Talaromyces stipitatus
38.9	-	4-nitrophenyl 2-O-(E)-feruloyl-alpha-L-arabinofuranoside	-	Talaromyces stipitatus
39.7	-	4-nitrophenyl 2-O-(E)-feruloyl-alpha-D-xylopyranoside	-	Talaromyces stipitatus
175	-	4-nitrophenyl 5-O-(E)-feruloyl-alpha-L-arabinofuranoside	-	Aspergillus niger