Literature summary for 3.1.1.73 extracted from

Buanafina, M.M.; Langdon, T.; Hauck, B.; Dalton, S.; Timms-Taravella, E.; Morris, P.
Targeting expression of a fungal ferulic acid esterase to the apoplast, endoplasmic reticulum or golgi can disrupt feruloylation of the growing cell wall and increase the biodegradability of tall fescue (Festuca arundinacea) (2010), Plant Biotechnol. J., 8, 316-331.

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Application

Application	Comment	Organism
industry	targeting FAEA expression to the Golgi or apoplast is likely to be an effective strategy for improving wall digestibility in grass species used for fodder or cellulosic ethanol production	Aspergillus niger

Cloned(Commentary)

Cloned (Comment)	Organism
transgenic Festuca arundinacea lines expressing the faeA gene under the rice actin, heat-shock and senescence promoters	Aspergillus niger

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Organism

Organism	UniProt	Comment	Textmining
Aspergillus niger	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	up to 23% of the total FAE activity is found in detergent extracts of the residues of leaf tissue of plants transformed with FAEA targeted to the endoplasmic reticulum or Golgi, particularly those plants which show higher activity in soluble extracts such as plants T36 and T38. 50%60% of the FAEA activity is found in apoplast extracts	Aspergillus niger	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ethyl ferulate + H2O	-	Aspergillus niger	ferulic acid + ethanol	-	?
additional information	release of cell wall hydroxycinnamic acids is enhanced by FAEA expression and this is amplified by exogenously applied xylanase. Release of cell wall sugars is enhanced by FAEA expression in the presence of exogenously applied cellulase. Highest FAEA-expressing plants show low ferulate levels and increased sugar release, compared with control plants, but no significant change in acid detergent lignin or in neutral or acid detergent fibre	Aspergillus niger	?	-	?

Synonyms

Synonyms	Comment	Organism
FAEA	-	Aspergillus niger
ferulic acid esterase	-	Aspergillus niger

Expression

Organism	Comment	Expression
Aspergillus niger	expression of the faeA gene is much lower with the native fungal Aspergillus apoplast sequence	down
Aspergillus niger	expression of the faeA gene under the control of the rice actin promoter, is highest when FAEA is targeted to the apoplast with the plant-derived N-terminal apoplast targeting motifs from the potato protease inhibitor or with the mutated barley aleurain vacuole signal sequence. Endoplasmic reticulum-targeted and Golgi-targeted plants show intermediate FAE activities. FAE activity varies with leaf age, with the highest activities in young leaves	up

General Information

General Information	Comment	Organism
physiological function	endpoint in vitro-dry-matter-digestibility is enhanced by FAEA expression in some individual plants. Initial rates of cell wall fermentation are enhanced under rumen conditions by FAEA expression	Aspergillus niger

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Release 2023.1 (January 2023)