Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Pichia pastoris strain KM71 | Aspergillus niger |
Protein Variants | Comment | Organism |
---|---|---|
D174A | site-directed mutagenesis, the mutant does not show changes in thermal stability compared to the wild-type enzyme | Aspergillus niger |
D93G | site-directed mutagenesis, the mutant shows slightly increased thermal stability compared to the wild-type enzyme | Aspergillus niger |
S187F | site-directed mutagenesis, the mutant shows highly increased thermal stability compared to the wild-type enzyme. The substitution of S187F reduces three possible hydrogen bonds | Aspergillus niger |
S92A | site-directed mutagenesis, the mutant does not show changes in thermal stability compared to the wild-type enzyme | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics, overview | Aspergillus niger | |
12.87 | - |
ferulate | pH 6.4, 40°C, mutant D93G | Aspergillus niger | |
14.4 | - |
ferulate | pH 6.4, 40°C, wild-type enzyme | Aspergillus niger | |
20.1 | - |
ferulate | pH 6.4, 40°C, mutant S187F | Aspergillus niger | |
23.35 | - |
ferulate | pH 6.4, 40°C, mutant D93G/S187F | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | O42807 | - |
- |
Aspergillus niger CIB 423.1 | O42807 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
29.5 | - |
wild-type enzyme, substrate 4-nitrophenyl ferulate, pH 6.4, 40°C | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl ferulate + H2O | - |
Aspergillus niger | 4-nitrophenol + ferulate | - |
? | |
4-nitrophenyl ferulate + H2O | - |
Aspergillus niger CIB 423.1 | 4-nitrophenol + ferulate | - |
? | |
methyl ferulate + H2O | - |
Aspergillus niger | ferulic acid + methanol | - |
? | |
methyl ferulate + H2O | - |
Aspergillus niger CIB 423.1 | ferulic acid + methanol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FAEA | - |
Aspergillus niger |
feruloyl esterase A | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
assay at | Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
half-lives of wild-type enzyme and mutants S92A and D174A is 8 min, half-life of mutant D93G is 9.4 min, and of mutant S187F 60.5 min | Aspergillus niger |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
62 | - |
ferulate | pH 6.4, 40°C, wild-type enzyme | Aspergillus niger | |
84 | - |
ferulate | pH 6.4, 40°C, mutant D93G | Aspergillus niger | |
790 | - |
ferulate | pH 6.4, 40°C, mutant D93G/S187F | Aspergillus niger | |
823 | - |
ferulate | pH 6.4, 40°C, mutant S187F | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
assay at | Aspergillus niger |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.3 | - |
ferulate | pH 6.4, 40°C, wild-type enzyme | Aspergillus niger | |
6.5 | - |
ferulate | pH 6.4, 40°C, mutant D93G | Aspergillus niger | |
34 | - |
ferulate | pH 6.4, 40°C, mutant D93G/S187F | Aspergillus niger | |
41 | - |
ferulate | pH 6.4, 40°C, mutant S187F | Aspergillus niger |