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Literature summary for 3.1.1.73 extracted from
- Product patterns of a feruloyl esterase from Aspergillus nidulans on large feruloyl-arabino-xylo-oligosaccharides from wheat bran (2012), Biores. Technol., 119, 425-428.
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | the feruloyl esterase from Aspergillus nidulans can be useful for the enzymatic deconstruction of xylans in plant cell walls | Aspergillus nidulans |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged enzyme in Pichia pastoris | Aspergillus nidulans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.23 | 0.32 | feruloyl arabino-xylo-oligosaccharides | recombinant His-tagged enzyme, pH 7.0, 37°C | Aspergillus nidulans | |
| 0.96 | - |
methyl ferulate | recombinant His-tagged enzyme, pH 7.0, 37°C | Aspergillus nidulans |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus nidulans | Q5B2G3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Pichia pastoris by ammonium sulfate fractionation and metal chelating affinity chromatography, to homogeneity | Aspergillus nidulans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 20.3 | 25.4 | substrate feruloyl arabino-xylo-oligosaccharides, recombinant His-tagged enzyme, pH 7.0, 37°C | Aspergillus nidulans |
| 21.7 | - |
substrate methyl ferulate, recombinant His-tagged enzyme, pH 7.0, 37°C | Aspergillus nidulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| feruloyl arabino-xylo-oligosaccharides + H2O | from wheat bran | Aspergillus nidulans | ? | product pattern, overview | ? | |
| methyl ferulate + H2O | - |
Aspergillus nidulans | methanol + ferulate | - |
? | |
| additional information | the feruloyl esterase shows similar specific activity but an affinity about 3.5fold higher towards feruloyl oligosaccharides than towards methyl ferulate. The esterase is able to hydrolyze the largest feruloyl oligosaccharides and therefore can act alone on feruloyled xylans | Aspergillus nidulans | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FAE | - |
Aspergillus nidulans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Aspergillus nidulans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9.5 | 11.8 | feruloyl arabino-xylo-oligosaccharides | recombinant His-tagged enzyme, pH 7.0, 37°C | Aspergillus nidulans | |
| 10.1 | - |
methyl ferulate | recombinant His-tagged enzyme, pH 7.0, 37°C | Aspergillus nidulans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Aspergillus nidulans |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10.5 | - |
methyl ferulate | recombinant His-tagged enzyme, pH 7.0, 37°C | Aspergillus nidulans | |
| 29.7 | 51.3 | feruloyl arabino-xylo-oligosaccharides | recombinant His-tagged enzyme, pH 7.0, 37°C | Aspergillus nidulans |
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