Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Aspergillus terreus | |
Fe2+ | - |
Aspergillus terreus | |
Fe3+ | - |
Aspergillus terreus | |
Hg2+ | complete inhibition at 1 mM; complete inhibition at 1 mM | Aspergillus terreus | |
K+ | - |
Aspergillus terreus | |
Mg2+ | - |
Aspergillus terreus | |
PMSF | complete inhibition at 1 mM; complete inhibition at 1 mM | Aspergillus terreus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Ca2+ has no significant effect on the enzyme activity | Aspergillus terreus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
x * 23000, SDS-PAGE | Aspergillus terreus |
36000 | - |
x * 36000, SDS-PAGE | Aspergillus terreus |
76000 | - |
x * 76000, SDS-PAGE | Aspergillus terreus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus terreus | - |
isolated from the soils of agricultural fields cultivating sweet sorghum, type C isozyme AtFAE-2 | - |
Aspergillus terreus | - |
isolated from the soils of agricultural fields cultivating sweet sorghum, type C isozyme AtFAE-3 | - |
Aspergillus terreus | Q0CBM7 | isolated from the soils of agricultural fields cultivating sweet sorghum, type A isozyme AtFAE-1 | - |
Aspergillus terreus MTCC 11096 | - |
isolated from the soils of agricultural fields cultivating sweet sorghum, type C isozyme AtFAE-2 | - |
Aspergillus terreus MTCC 11096 | - |
isolated from the soils of agricultural fields cultivating sweet sorghum, type C isozyme AtFAE-3 | - |
Aspergillus terreus MTCC 11096 | Q0CBM7 | isolated from the soils of agricultural fields cultivating sweet sorghum, type A isozyme AtFAE-1 | - |
Purification (Comment) | Organism |
---|---|
native isozyme AtFAE-1 67fold from strain MTCC 11096 by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography | Aspergillus terreus |
native isozyme AtFAE-2 58fold from strain MTCC 11096 by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, and gel filtration | Aspergillus terreus |
native isozyme AtFAE-3 56fold from strain MTCC 11096 by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, and gel filtration | Aspergillus terreus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
263.3 | - |
purified isozyme, pH 5.0, 40°C | Aspergillus terreus |
275.5 | - |
purified isozyme, pH 5.0, 40°C | Aspergillus terreus |
317.7 | - |
purified isozyme, pH 5.0, 40°C | Aspergillus terreus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl ferulate + H2O | - |
Aspergillus terreus | methanol + ferulate | - |
? | |
methyl ferulate + H2O | - |
Aspergillus terreus MTCC 11096 | methanol + ferulate | - |
? | |
additional information | release of ferulic acid from destarched maize bran, substrate specificity of isozyme AtFAE-1 for diverse ester substrates, overview | Aspergillus terreus | ? | - |
? | |
additional information | release of ferulic acid from destarched maize bran, substrate specificity of isozyme AtFAE-2 for diverse ester substrates, overview | Aspergillus terreus | ? | - |
? | |
additional information | release of ferulic acid from destarched maize bran, substrate specificity of isozyme AtFAE-3 for diverse ester substrates, overview | Aspergillus terreus | ? | - |
? | |
additional information | release of ferulic acid from destarched maize bran, substrate specificity of isozyme AtFAE-2 for diverse ester substrates, overview | Aspergillus terreus MTCC 11096 | ? | - |
? | |
additional information | release of ferulic acid from destarched maize bran, substrate specificity of isozyme AtFAE-3 for diverse ester substrates, overview | Aspergillus terreus MTCC 11096 | ? | - |
? | |
additional information | release of ferulic acid from destarched maize bran, substrate specificity of isozyme AtFAE-1 for diverse ester substrates, overview | Aspergillus terreus MTCC 11096 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 76000, SDS-PAGE | Aspergillus terreus |
? | x * 36000, SDS-PAGE | Aspergillus terreus |
? | x * 23000, SDS-PAGE | Aspergillus terreus |
Synonyms | Comment | Organism |
---|---|---|
AtFAE-1 | - |
Aspergillus terreus |
AtFAE-2 | - |
Aspergillus terreus |
AtFAE-3 | - |
Aspergillus terreus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Aspergillus terreus |
50 | - |
- |
Aspergillus terreus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified isozyme AtFAE-2, 4 h incubation at pH 3.0-8.0, over 50% activity remaining | Aspergillus terreus |
40 | - |
purified isozyme AtFAE-3, 4 h incubation at pH 3.0-8.0, over 50% activity remaining | Aspergillus terreus |
50 | - |
purified isozyme AtFAE-1, 4 h incubation at pH 3.0-8.0, over 50% activity remaining | Aspergillus terreus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Aspergillus terreus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | over 50% of maximal activity within this range | Aspergillus terreus |
5 | 6 | over 50% of maximal activity within this range | Aspergillus terreus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | purified isozyme AtFAE-1, 4 h incubation at 50°C, over 50% activity remaining. After 12 h incubation, the enzyme is more stable in acidic to neutral conditions than in alkaline conditions | Aspergillus terreus |
3 | 8 | purified isozyme AtFAE-2, 4 h incubation at 40°C, over 50% activity remaining. After 12 h incubation, the enzyme is more stable in acidic to neutral conditions than in alkaline conditions | Aspergillus terreus |
3 | 8 | purified isozyme AtFAE-3, 4 h incubation at 50°C, over 50% activity remaining. After 12 h incubation, the enzyme is more stable in acidic to neutral conditions than in alkaline conditions | Aspergillus terreus |