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Literature summary for 3.1.1.73 extracted from

  • Uraji, M.; Arima, J.; Inoue, Y.; Harazono, K.; Hatanaka, T.
    Application of two newly identified and characterized feruloyl esterases from Streptomyces sp. in the enzymatic production of ferulic acid from agricultural biomass (2014), PLoS ONE, 9, e104584.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.73

Inhibitors

Inhibitors Comment Organism Structure
Co2+
-
 Streptomyces cinnamoneus
Fe3+ strong inhibition of isozyme R18, weak inhibition of isozyme R43 Streptomyces cinnamoneus
Ni2+
-
 Streptomyces cinnamoneus
PMSF
-
 Streptomyces cinnamoneus
Zn2+ strong inhibition of both isozymes Streptomyces cinnamoneus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
 4-nitrophenyl butyrate isozyme R43, pH 7.0, 50°C Streptomyces cinnamoneus
0.54
-
 methyl sinapinate isozyme R43, pH 7.0, 50°C Streptomyces cinnamoneus
1.96
-
 ethyl ferulate isozyme R43, pH 7.0, 50°C Streptomyces cinnamoneus
2.61
-
 methyl caffeate isozyme R43, pH 7.0, 50°C Streptomyces cinnamoneus
3
-
 methyl 4-coumarate isozyme R43, pH 7.0, 50°C Streptomyces cinnamoneus
3.31
-
 methyl caffeate isozyme R18, pH 7.0, 50°C Streptomyces cinnamoneus
4.28
-
 ethyl ferulate isozyme R18, pH 7.0, 50°C Streptomyces cinnamoneus
4.31
-
 methyl 4-coumarate isozyme R18, pH 7.0, 50°C Streptomyces cinnamoneus
4.41
-
 methyl ferulate isozyme R43, pH 7.0, 50°C Streptomyces cinnamoneus
4.99
-
 methyl ferulate isozyme R18, pH 7.0, 50°C Streptomyces cinnamoneus
9.4
-
 methyl sinapinate isozyme R18, pH 7.0, 50°C Streptomyces cinnamoneus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the R18 sequence contains a signal sequence at the N-terminal. Although R43 has no signal peptide for secretion, the enzyme is secreted by the Streptomyces protein expression system Streptomyces cinnamoneus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 x * 38000, extracellular isozyme R18, SDS-PAGE, x * 52000, extracellular isozyme R43, SDS-PAGE Streptomyces cinnamoneus
52000
-
 x * 38000, extracellular isozyme R18, SDS-PAGE, x * 52000, extracellular isozyme R43, SDS-PAGE Streptomyces cinnamoneus

Organism

Organism UniProt Comment Textmining
Streptomyces cinnamoneus
-
 isozymes R18 and R43 identified from screening of feruloyl esterases from a Streptomyces esterase library, overview
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.07
-
 purified recombinant isozyme R18, pH 7.0, 50°C, substrate 4-nitrophenylbutyrate Streptomyces cinnamoneus
0.37
-
 purified recombinant isozyme R43, pH 7.0, 50°C, substrate methyl vanillate Streptomyces cinnamoneus
0.49
-
 purified recombinant isozyme R43, pH 7.0, 50°C, substrate 4-nitrophenylbutyrate Streptomyces cinnamoneus
1.1
-
 purified recombinant isozyme R43, pH 7.0, 50°C, substrate methyl sinapinate Streptomyces cinnamoneus
1.54
-
 purified recombinant isozyme R43, pH 7.0, 50°C, substrate methyl caffeate Streptomyces cinnamoneus
1.89
-
 purified recombinant isozyme R18, pH 7.0, 50°C, substrate methyl vanillate Streptomyces cinnamoneus
5.4
-
 purified recombinant isozyme R18, pH 7.0, 50°C, substrate methyl caffeate Streptomyces cinnamoneus
6.75
-
 purified recombinant isozyme R43, pH 7.0, 50°C, substrate methyl 4-coumarate Streptomyces cinnamoneus
10.02
-
 purified recombinant isozyme R43, pH 7.0, 50°C, substrate ethyl ferulate Streptomyces cinnamoneus
10.9
-
 purified recombinant isozyme R18, pH 7.0, 50°C, substrate methyl sinapinate Streptomyces cinnamoneus
13.75
-
 purified recombinant isozyme R18, pH 7.0, 50°C, substrate methyl 4-coumarate Streptomyces cinnamoneus
18.97
-
 purified recombinant isozyme R18, pH 7.0, 50°C, substrate ethyl ferulate Streptomyces cinnamoneus
19.8
-
 purified recombinant isozyme R43, pH 7.0, 50°C, substrate methyl ferulate Streptomyces cinnamoneus
23.07
-
 purified recombinant isozyme R18, pH 7.0, 50°C, substrate methyl ferulate Streptomyces cinnamoneus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl butyrate + H2O
-
 Streptomyces cinnamoneus 4-nitrophenol + butyrate
-
 ?
ethyl ferulate + H2O
-
 Streptomyces cinnamoneus ethanol + ferulate
-
 ?
methyl 4-coumarate + H2O
-
 Streptomyces cinnamoneus 4-coumarate + methanol
-
 ?
methyl caffeate + H2O
-
 Streptomyces cinnamoneus caffeic acid + methanol
-
 ?
methyl ferulate + H2O
-
 Streptomyces cinnamoneus methanol + ferulate
-
 ?
methyl sinapinate + H2O
-
 Streptomyces cinnamoneus sinapate + methanol
-
 ?
methyl vanillate + H2O
-
 Streptomyces cinnamoneus vanillate + methanol
-
 ?
additional information two isozymes from Streptomyces cinnamoneus, R18 and R43, individually show the ability to produce ferulate from corn bran. Combination with other Streptomyces enzymes, namely xylanase and alpha-L-arabinofuranosidase, increases ferulate production from biomass such as corn bran, defatted rice bran, and wheat bran Streptomyces cinnamoneus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 38000, extracellular isozyme R18, SDS-PAGE, x * 52000, extracellular isozyme R43, SDS-PAGE Streptomyces cinnamoneus

Synonyms

Synonyms Comment Organism
FAE
-
 Streptomyces cinnamoneus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 isozyme R43 Streptomyces cinnamoneus
50
-
 isozyme R18 Streptomyces cinnamoneus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 50 activity range, isozyme R43 Streptomyces cinnamoneus
20 50 activity range, isozyme R18 Streptomyces cinnamoneus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 40 isozyme R43, thermolabile, 30 min, loss of 50% activity at 25°C, inactivation at 40°C Streptomyces cinnamoneus
50 60 isozyme R18, 30 min, completely stable up to 50°C, inactivation at 60°C Streptomyces cinnamoneus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 isozyme R43 Streptomyces cinnamoneus
7.5 8 isozyme R18 Streptomyces cinnamoneus

pH Range

pH Minimum pH Maximum Comment Organism
5 9 activity range, isozymes R18 and R43 Streptomyces cinnamoneus

General Information

General Information Comment Organism
physiological function feruloyl esterase is a key bacterial enzyme involved in ferulate production from agricultural biomass Streptomyces cinnamoneus