Any feedback?
Literature summary for 3.1.1.73 extracted from
- Enhancing the thermostability of feruloyl esterase EstF27 by directed evolution and the underlying structural basis (2015), J. Agric. Food Chem., 63, 8225-8233.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| T27I/S84L/V161I/G243C | mutant displays significantly improved thermostability | uncultured bacterium |
| T27I/S84L/V161I/H195L/G243C/A259V | mutant displays significantly improved thermostability | uncultured bacterium |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.36 | - |
4-nitrophenyl ferulate | mutant T27I/S84L/V161I/H195L/G243C/A259V, pH 7.0, 40°C | uncultured bacterium |  |
| 0.42 | - |
4-nitrophenyl ferulate | mutant T27I/S84L/V161I/H195L/G243C/A259V, pH 7.0, 65°C | uncultured bacterium | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| uncultured bacterium | E7DJY5 | - |
- |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 25°C, storage for 150 days, the activity of mutant T27I/S84L/V161I/H195L/G243C/A259V remains almost constant (100.5%), whereas that of the wild-type is only 21.2% | uncultured bacterium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl ferulate + H2O | - |
uncultured bacterium | ferulic acid + 4-nitrophenol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EstF27 | - |
uncultured bacterium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
wild-type | uncultured bacterium |
| 60 | - |
mutant T27I/S84L/V161I/G243C | uncultured bacterium |
| 65 | - |
mutant T27I/S84L/V161I/H195L/G243C/A259V | uncultured bacterium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
half-life of wild-type 0.5 h, mutant T27I/S84L/V161I/G243C 70 h, mutant T27I/S84L/V161I/H195L/G243C/A259V 1680 h, respectively | uncultured bacterium |
| 65 | - |
half-life of mutant T27I/S84L/V161I/G243C 4 h, mutant T27I/S84L/V161I/H195L/G243C/A259V 140 h, respectively | uncultured bacterium |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 18.9 | - |
4-nitrophenyl ferulate | mutant T27I/S84L/V161I/H195L/G243C/A259V, pH 7.0, 40°C | uncultured bacterium | |
| 32.2 | - |
4-nitrophenyl ferulate | mutant T27I/S84L/V161I/H195L/G243C/A259V, pH 7.0, 65°C | uncultured bacterium | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 52.4 | - |
4-nitrophenyl ferulate | mutant T27I/S84L/V161I/H195L/G243C/A259V, pH 7.0, 40°C | uncultured bacterium | |
| 76.6 | - |
4-nitrophenyl ferulate | mutant T27I/S84L/V161I/H195L/G243C/A259V, pH 7.0, 65°C | uncultured bacterium | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
