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Literature summary for 3.1.1.73 extracted from
- Acidic-alkaline ferulic acid esterase from Chaetomium thermophilum var. dissitum Molecular cloning and characterization of recombinant enzyme expressed in Pichia pastoris (2016), Biocatal. Agricult. Biotechnol., 5, 48-55 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris KM71H. | Thermochaetoides dissita |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.016 | - |
methyl p-coumarate | pH 6.0, 55°C | Thermochaetoides dissita |  |
| 0.025 | - |
ethyl ferulate | pH 6.0, 55°C | Thermochaetoides dissita | |
| 0.031 | - |
methyl caffeate | pH 6.0, 55°C | Thermochaetoides dissita | |
| 0.25 | - |
1-naphthyl acetate | pH 6.0, 55°C | Thermochaetoides dissita | |
| 0.54 | - |
4-nitrophenyl butanoate | pH 6.0, 55°C | Thermochaetoides dissita | |
| 0.7 | - |
4-nitrophenyl acetate | pH 6.0, 55°C | Thermochaetoides dissita | |
| 0.8 | - |
4-nitrophenyl ferulate | pH 6.0, 55°C | Thermochaetoides dissita | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermochaetoides dissita | A0A1B1LML0 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-naphthyl acetate + H2O | - |
Thermochaetoides dissita | 1-naphthol + acetate | - |
? | |
| 4-nitrophenyl acetate + H2O | - |
Thermochaetoides dissita | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl butanoate + H2O | - |
Thermochaetoides dissita | 4-nitrophenol + butanoate | - |
? | |
| 4-nitrophenyl ferulate + H2O | - |
Thermochaetoides dissita | 4-nitrophenol + ferulate | - |
? | |
| arabinoxylan + H2O | - |
Thermochaetoides dissita | ferulic acid + ? | - |
? | |
| ethyl ferulate + H2O | - |
Thermochaetoides dissita | ethanol + ferulate | - |
? | |
| methyl caffeate + H2O | - |
Thermochaetoides dissita | methanol + caffeate | - |
? | |
| methyl p-coumarate + H2O | - |
Thermochaetoides dissita | methanol + p-coumarate | - |
? | |
| additional information | Fae is active towards synthetic esters of ferulic, p-coumaric, and caffeic acids, as well as towards wide range of 4-nitrophenyl substrates | Thermochaetoides dissita | ? | - |
? | |
| rye grass + H2O | - |
Thermochaetoides dissita | ferulic acid + p-coumaric acid + ? | - |
? | |
| wheat straw + H2O | - |
Thermochaetoides dissita | ferulic acid + p-coumaric acid + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 29600, calculated, x * 30000, SDS-PAGE | Thermochaetoides dissita |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FAE | - |
Thermochaetoides dissita |
| ferulic acid esterase | - |
Thermochaetoides dissita |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Thermochaetoides dissita |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 65 | more than 70% of the maximal activity | Thermochaetoides dissita |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
24 h, 99% residual activity | Thermochaetoides dissita |
| 50 | - |
24 h, 98% residual activity | Thermochaetoides dissita |
| 60 | - |
24 h, 73% residual activity | Thermochaetoides dissita |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.1 | - |
methyl caffeate | pH 6.0, 55°C | Thermochaetoides dissita | |
| 4.1 | - |
ethyl ferulate | pH 6.0, 55°C | Thermochaetoides dissita | |
| 5.8 | - |
methyl p-coumarate | pH 6.0, 55°C | Thermochaetoides dissita | |
| 24 | - |
4-nitrophenyl ferulate | pH 6.0, 55°C | Thermochaetoides dissita | |
| 32 | - |
1-naphthyl acetate | pH 6.0, 55°C | Thermochaetoides dissita | |
| 59 | - |
4-nitrophenyl butanoate | pH 6.0, 55°C | Thermochaetoides dissita | |
| 60 | - |
4-nitrophenyl acetate | pH 6.0, 55°C | Thermochaetoides dissita | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Thermochaetoides dissita |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | more than 80% of maximal activity | Thermochaetoides dissita |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | 4°C, 24 h, 70% residual activity | Thermochaetoides dissita |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Thermochaetoides dissita | calculated | - |
6.8 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 30 | - |
4-nitrophenyl ferulate | pH 6.0, 55°C | Thermochaetoides dissita | |
| 86 | - |
4-nitrophenyl acetate | pH 6.0, 55°C | Thermochaetoides dissita | |
| 99 | - |
methyl caffeate | pH 6.0, 55°C | Thermochaetoides dissita | |
| 109 | - |
4-nitrophenyl butanoate | pH 6.0, 55°C | Thermochaetoides dissita | |
| 128 | - |
1-naphthyl acetate | pH 6.0, 55°C | Thermochaetoides dissita | |
| 164 | - |
ethyl ferulate | pH 6.0, 55°C | Thermochaetoides dissita | |
| 363 | - |
methyl p-coumarate | pH 6.0, 55°C | Thermochaetoides dissita | |
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