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Literature summary for 3.1.1.73 extracted from
- Loop of Streptomyces feruloyl esterase plays an important role in the enzymes catalyzing the release of ferulic acid from biomass (2018), Appl. Environ. Microbiol., 84, e02300 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D214A | mutation within catalytic triad, complete loss of activity | Streptomyces cinnamoneus |
| H268A | mutation within catalytic triad, complete loss of activity | Streptomyces cinnamoneus |
| additional information | construction of chimeric proteins by RIBS in vivo DNA shuffling of the feruloyl esterase genes of strains Streptomyces cinnamoneus NBRC 12852 and Streptomyces cinnamoneus TH-2. A domain comprised of residues 140 to 154 is crucial for the catalytic activity of isoform EstA | Streptomyces cinnamoneus |
| additional information | construction of chimeric proteins by RIBS in vivo DNA shuffling of the feruloyl esterase genes of strains Streptomyces cinnamoneus NBRC 12852 and Streptomyces cinnamoneus TH-2. insertion of residues Pro and Gly between residues Asn-152 and Ala-153, i.e. mutant 18PG, leads to increased activity with substrates methyl ferulate and methyl sinapinate | Streptomyces sp. TH-2 |
| S191A | mutation within catalytic triad, complete loss of activity | Streptomyces cinnamoneus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.63 | - |
methyl sinapinate | wild-type, pH not specified in the publication, 50°C | Streptomyces sp. TH-2 |  |
| 2 | 77 | methyl sinapinate | mutant 18PG, pH not specified in the publication, 50°C | Streptomyces sp. TH-2 | |
| 2.56 | - |
methyl sinapinate | pH not specified in the publication, 50°C | Streptomyces cinnamoneus | |
| 2.77 | - |
methyl ferulate | mutant 18PG, pH not specified in the publication, 50°C | Streptomyces sp. TH-2 | |
| 3.72 | - |
methyl ferulate | pH not specified in the publication, 50°C | Streptomyces cinnamoneus | |
| 4.13 | - |
ethyl ferulate | mutant 18PG, pH not specified in the publication, 50°C | Streptomyces sp. TH-2 | |
| 4.44 | - |
ethyl ferulate | wild-type, pH not specified in the publication, 50°C | Streptomyces sp. TH-2 | |
| 5.59 | - |
ethyl ferulate | pH not specified in the publication, 50°C | Streptomyces cinnamoneus | |
| 9.66 | - |
methyl ferulate | wild-type, pH not specified in the publication, 50°C | Streptomyces sp. TH-2 | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces cinnamoneus | A0A0M4UW33 | - |
- |
| Streptomyces cinnamoneus NBRC 12852 | A0A0M4UW33 | - |
- |
| Streptomyces sp. TH-2 | A0A1E1FNX1 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethyl ferulate + H2O | - |
Streptomyces cinnamoneus | ferulate + ethanol | - |
? | |
| ethyl ferulate + H2O | - |
Streptomyces sp. TH-2 | ferulate + ethanol | - |
? | |
| ethyl ferulate + H2O | - |
Streptomyces cinnamoneus NBRC 12852 | ferulate + ethanol | - |
? | |
| methyl ferulate + H2O | - |
Streptomyces cinnamoneus | ferulate + methanol | - |
? | |
| methyl ferulate + H2O | - |
Streptomyces sp. TH-2 | ferulate + methanol | - |
? | |
| methyl ferulate + H2O | - |
Streptomyces cinnamoneus NBRC 12852 | ferulate + methanol | - |
? | |
| methyl sinapinate + H2O | - |
Streptomyces cinnamoneus | sinapinate + methanol | - |
? | |
| methyl sinapinate + H2O | - |
Streptomyces sp. TH-2 | sinapinate + methanol | - |
? | |
| methyl sinapinate + H2O | - |
Streptomyces cinnamoneus NBRC 12852 | sinapinate + methanol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EstA | - |
Streptomyces cinnamoneus |
| R18 | - |
Streptomyces cinnamoneus |
| TH2-18 | - |
Streptomyces sp. TH-2 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Streptomyces cinnamoneus |
| 45 | - |
- |
Streptomyces sp. TH-2 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
30 min, complete loss of activity | Streptomyces cinnamoneus |
| 60 | - |
30 min, complete loss of activity | Streptomyces sp. TH-2 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0008 | - |
methyl sinapinate | wild-type, pH not specified in the publication, 50°C | Streptomyces sp. TH-2 | |
| 0.0017 | - |
methyl sinapinate | pH not specified in the publication, 50°C | Streptomyces cinnamoneus | |
| 0.0027 | - |
ethyl ferulate | wild-type, pH not specified in the publication, 50°C | Streptomyces sp. TH-2 | |
| 0.0045 | - |
methyl ferulate | wild-type, pH not specified in the publication, 50°C | Streptomyces sp. TH-2 | |
| 0.0145 | - |
methyl ferulate | mutant 18PG, pH not specified in the publication, 50°C | Streptomyces sp. TH-2 | |
| 0.0283 | - |
methyl ferulate | pH not specified in the publication, 50°C | Streptomyces cinnamoneus | |
| 0.0422 | - |
ethyl ferulate | pH not specified in the publication, 50°C | Streptomyces cinnamoneus | |
| 0.073 | - |
ethyl ferulate | mutant 18PG, pH not specified in the publication, 50°C | Streptomyces sp. TH-2 | |
| 0.0732 | - |
methyl sinapinate | mutant 18PG, pH not specified in the publication, 50°C | Streptomyces sp. TH-2 | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
and 8.0 | Streptomyces cinnamoneus |
| 6.5 | - |
and 8.0 | Streptomyces sp. TH-2 |
| 8 | - |
and 6.5 | Streptomyces cinnamoneus |
| 8 | - |
and 6.5 | Streptomyces sp. TH-2 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00047 | - |
methyl ferulate | wild-type, pH not specified in the publication, 50°C | Streptomyces sp. TH-2 | |
| 0.0006 | - |
methyl sinapinate | pH not specified in the publication, 50°C | Streptomyces cinnamoneus | |
| 0.0006 | - |
methyl sinapinate | wild-type, pH not specified in the publication, 50°C | Streptomyces sp. TH-2 | |
| 0.00062 | - |
ethyl ferulate | wild-type, pH not specified in the publication, 50°C | Streptomyces sp. TH-2 | |
| 0.00528 | - |
methyl ferulate | mutant 18PG, pH not specified in the publication, 50°C | Streptomyces sp. TH-2 | |
| 0.0076 | - |
ethyl ferulate | pH not specified in the publication, 50°C | Streptomyces cinnamoneus | |
| 0.0077 | - |
methyl ferulate | pH not specified in the publication, 50°C | Streptomyces cinnamoneus | |
| 0.0177 | - |
ethyl ferulate | mutant 18PG, pH not specified in the publication, 50°C | Streptomyces sp. TH-2 | |
| 0.0427 | - |
methyl sinapinate | mutant 18PG, pH not specified in the publication, 50°C | Streptomyces sp. TH-2 | |
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