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Literature summary for 3.1.1.73 extracted from

  • Kelle, S.; Nieter, A.; Krings, U.; Zelena, K.; Linke, D.; Berger, R.
    Heterologous production of a feruloyl esterase from Pleurotus sapidus synthesizing feruloyl-saccharide esters (2016), Biotechnol. Appl. Biochem., 63, 852-862 .
    View publication on PubMed
Search for more literature for 3.1.1.73

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli and Pichia pastoris Pleurotus sapidus

Protein Variants

Protein Variants Comment Organism
additional information expression in Pichia pastoris with an N-terminal alpha-mating factor pre-pro sequence and under the control of a methanol inducible alcohol oxidase 1 promotor Pleurotus sapidus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.227
-
 beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose pH 5.0, 37°C Pleurotus sapidus
0.427
-
 methyl ferulate pH 5.0, 37°C Pleurotus sapidus

Organism

Organism UniProt Comment Textmining
Pleurotus sapidus D5GRP3
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein molecular mass of deglycosylated protein is around 60000 Da on SDS-PAGE and 59400, calculated. Molecular mass of glycosylated protein is around 70000 Da Pleurotus sapidus

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Pleurotus sapidus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1027
-
 substrate methyl ferulate, pH 5.0, 37°C Pleurotus sapidus
1681
-
 substrate beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose, pH 5.0, 37°C Pleurotus sapidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-O-(trans-feruloyl)-L-arabinofuranose + H2O 83% of the activity with beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose Pleurotus sapidus ferulic acid + L-arabinose
-
 ?
alpha-L-galactopyranosyl-(1->2)-beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose + H2O 48.4% of the activity with beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose Pleurotus sapidus alpha-L-galactopyranosyl-(1->2)-beta-D-xylopyranosyl-(1->2)-L-arabinofuranose + ferulate
-
 ?
beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose + H2O
-
 Pleurotus sapidus beta-D-xylopyranosyl-(1->2)-L-arabinofuranose + ferulate
-
 ?
destarched wheat bran + H2O
-
 Pleurotus sapidus ferulate + ?
-
 ?
ethyl ferulate + H2O 61.1% of the activity with beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose Pleurotus sapidus ferulate + ethanol
-
 ?
methyl ferulate + H2O 62.6% of the activity with beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose Pleurotus sapidus ferulate + methanol
-
 ?
methyl sinapinate + H2O 22.4% of the activity with beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose Pleurotus sapidus sinapinate + methanol
-
 ?
additional information enzyme catalyzes transesterification of methyl ferulate using D-glucose, D-fructose, and D-galactose and D-sucrose or D-maltose Pleurotus sapidus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 70000, SDS-PAGE, recombinant protein, x * 60000, SDS-PAGE of Endo H treated protein Pleurotus sapidus

Synonyms

Synonyms Comment Organism
Est1
-
 Pleurotus sapidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Pleurotus sapidus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2
-
 methyl ferulate pH 5.0, 37°C Pleurotus sapidus
1.96
-
 beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose pH 5.0, 37°C Pleurotus sapidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 recombinant protein Pleurotus sapidus
6
-
 native protein Pleurotus sapidus

pH Range

pH Minimum pH Maximum Comment Organism
3 8 no activity below/above Pleurotus sapidus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.81
-
 methyl ferulate pH 5.0, 37°C Pleurotus sapidus
8.64
-
 beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose pH 5.0, 37°C Pleurotus sapidus