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Literature summary for 3.1.1.73 extracted from

  • Gao, L.; Wang, M.; Chen, S.; Zhang, D.
    Biochemical characterization of a novel feruloyl esterase from Penicillium piceum and its application in biomass bioconversion (2016), J. Mol. Catal. B, 133, S388-S394 .
No PubMed abstract available

Application

Application Comment Organism
degradation low doses of enzyme (120 microg/g substrate) increases glucose yields released from corn stover, wheat bran, corn cob, and cassava stillage residues by 68.8%, 38.6%, 15.6%, and 20.0%, respectively Talaromyces piceae

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Talaromyces piceae

Crystallization (Commentary)

Crystallization (Comment) Organism
molecular modeling of structure based on PDB entry 3WMT Talaromyces piceae

Organism

Organism UniProt Comment Textmining
Talaromyces piceae A0A1S5XVA5
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
22
-
substrate methyl ferulate, pH 3.0, 70°C Talaromyces piceae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl caffeate + H2O
-
Talaromyces piceae caffeate + methanol
-
?
methyl ferulate + H2O
-
Talaromyces piceae ferulate + methanol
-
?
methyl p-coumarate + H2O
-
Talaromyces piceae p-coumarate + methanol
-
?
methyl sinapinate + H2O
-
Talaromyces piceae sinapinate + methanol
-
?
additional information enzyme is active in degradation of corn stover, wheat bran, corn cob, and cassava stillage Talaromyces piceae ?
-
?

Subunits

Subunits Comment Organism
? x * 56300, calculated, x * 56000, SDS-PAGE Talaromyces piceae

Synonyms

Synonyms Comment Organism
carboxylic ester hydrolase
-
Talaromyces piceae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Talaromyces piceae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60
-
65% of maximum activity Talaromyces piceae
80
-
48% of maximum activity Talaromyces piceae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
half-life 150 min Talaromyces piceae
70
-
half-life 220 min Talaromyces piceae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
-
Talaromyces piceae

pH Range

pH Minimum pH Maximum Comment Organism
3 5 more than 96% of maximum activity Talaromyces piceae