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Literature summary for 3.1.1.75 extracted from

  • Bhatt, R.; Patel, K.; Trivedi, U.
    Purification and properties of extracellular poly(3-hydroxybutyrate) depolymerase produced by Aspergillus fumigatus 202 (2010), J. Polym. Environ., 18, 141-147.
No PubMed abstract available
Search for more literature for 3.1.1.75

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol inactivation of the purified enzyme Aspergillus fumigatus
Fe2+ 88% inhibition at 5 mM Aspergillus fumigatus
Hg2+ complete inhibition at 5 mM Aspergillus fumigatus
N-bromosuccinimide inactivation of the purified enzyme Aspergillus fumigatus
Triton X-100
-
 Aspergillus fumigatus
Tween 20
-
 Aspergillus fumigatus
Tween 80
-
 Aspergillus fumigatus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Aspergillus fumigatus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1.5fold activation at 5 mM Aspergillus fumigatus
Mg2+ 1.5fold activation at 5 mM Aspergillus fumigatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63744
-
 x * 63744, SDS-PAGE Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
poly(3-hydroxybutyrate) + H2O Aspergillus fumigatus
-
 ?
-
 ?
poly(3-hydroxybutyrate) + H2O Aspergillus fumigatus 202
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus
-
 a thermotolerant fungal soil isolate
-
Aspergillus fumigatus 202
-
 a thermotolerant fungal soil isolate
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein
-
 Aspergillus fumigatus

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 20.62fold by acetone precipitation and gel filtration Aspergillus fumigatus

Source Tissue

Source Tissue Comment Organism Textmining
additional information maximum PHB depolymerase production is obtained at the end of 48 h with initial medium pH 7.0 and 45°C in Bushnell Haas Minerals medium containing PHB as sole source of carbon Aspergillus fumigatus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 4.5512 mg PHB/min, purified enzyme Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(3-hydroxybutyrate) + H2O
-
 Aspergillus fumigatus ?
-
 ?
poly(3-hydroxybutyrate) + H2O
-
 Aspergillus fumigatus 202 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 63744, SDS-PAGE Aspergillus fumigatus

Synonyms

Synonyms Comment Organism
PHB depolymerase
-
 Aspergillus fumigatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Aspergillus fumigatus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 70 activity range Aspergillus fumigatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 the purified enzyme retains 56.01% of maximal activity up to 55°C Aspergillus fumigatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Aspergillus fumigatus

pH Range

pH Minimum pH Maximum Comment Organism
3 10 activity range Aspergillus fumigatus

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus fumigatus isoelectric focusing
-
 4.2