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Literature summary for 3.1.1.75 extracted from
- Poly(-beta-hydroxybutyrate) (PHB) depolymerase PHAZ Pen from Penicillium expansum purification, characterization and kinetic studies (2015), 3 Biotech, 5, 901-909 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | maximum production of polyhydroxybutanoate depolymerase (6 U/mL) by 72 h when grown in mineral salt medium containing 0.2 % w/v polyhydroxybutanoate, pH 5.0, at 30 °C and 200 rpm shaking conditions | Penicillium expansum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 1 mM, 41% inhibition | Penicillium expansum |  |
| dithiothreitol | 1 mM, complete inhibition | Penicillium expansum | |
| Fe2+ | 1 mM, 84% inhibition | Penicillium expansum | |
| Hg2+ | 1 mM, 82% inhibition | Penicillium expansum | |
| N-ethylmaleimide | 1 mM, complete inhibition | Penicillium expansum | |
| SDS | 10 mM, 86% inhibition | Penicillium expansum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
poly(2-hydroxybutanoate) | Km value 1.04 microg/ml, pH 5.0, 50°C | Penicillium expansum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Penicillium expansum | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium expansum | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Penicillium expansum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Penicillium expansum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1821 | - |
pH 5.0, 50°C | Penicillium expansum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no substrates: p-nitrophenyl fatty acids or polycaprolactone | Penicillium expansum | ? | - |
? | |
| poly(2-hydroxybutanoate) + H2O | - |
Penicillium expansum | 2-hydroxybutanoate + ? | - |
? | |
| poly(3-hydroxybutanoate-co-3-hydroxyvalerate) + H2O | - |
Penicillium expansum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 20000, SDS-PAGE | Penicillium expansum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Penicillium expansum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 50 | - |
Penicillium expansum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
1 h, 70-80% residual activity | Penicillium expansum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Penicillium expansum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6 | - |
Penicillium expansum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6 | - |
Penicillium expansum |
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