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Literature summary for 3.1.1.76 extracted from

  • Rhee, Y.H.; Kim, Y.H.; Shin, K.
    Characterization of an extracellular poly(3-hydroxyoctanoate) depolymerase from the marine isolate, Pseudomonas luteola M13-4 (2006), Enzyme Microb. Technol., 38, 529-535.
No PubMed abstract available
Search for more literature for 3.1.1.76

Inhibitors

Inhibitors Comment Organism Structure
Acetic anhydride 10 mM, 83% inhibition Pseudomonas luteola
dithiothreitol 10 mM, 12% inhibition Pseudomonas luteola
EDTA 1 mM, 57% inhibition Pseudomonas luteola
N-bromosuccinimide 10 mM, 84% inhibition Pseudomonas luteola
NEM 10 mM, 29% inhibition Pseudomonas luteola
phenylmethylsulfonyl fluoride 10 mM, 93% inhibition Pseudomonas luteola
Triton X-100 0.1%, 80% inhibition Pseudomonas luteola

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Pseudomonas luteola
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 gel filtration Pseudomonas luteola

Organism

Organism UniProt Comment Textmining
Pseudomonas luteola
-
-
-
Pseudomonas luteola M13-4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas luteola

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl dodecanoate + H2O 10.4% of the activity with p-nitrophenydecanoate Pseudomonas luteola 4-nitrophenol + dodecanoate
-
 ?
4-nitrophenyl dodecanoate + H2O 10.4% of the activity with p-nitrophenydecanoate Pseudomonas luteola M13-4 4-nitrophenol + dodecanoate
-
 ?
4-nitrophenyl hexanoate + H2O 58.4% of the activity with p-nitrophenydecanoate Pseudomonas luteola 4-nitrophenol + hexanoate
-
 ?
4-nitrophenyl hexanoate + H2O 58.4% of the activity with p-nitrophenydecanoate Pseudomonas luteola M13-4 4-nitrophenol + hexanoate
-
 ?
4-nitrophenyl octanoate + H2O 71.9% of the activity with p-nitrophenydecanoate Pseudomonas luteola 4-nitrophenol + octanoate
-
 ?
4-nitrophenyl octanoate + H2O 71.9% of the activity with p-nitrophenydecanoate Pseudomonas luteola M13-4 4-nitrophenol + octanoate
-
 ?
additional information no activity with polycaprolactone and poly(3-hydroxybutyrate) Pseudomonas luteola ?
-
 ?
additional information no activity with polycaprolactone and poly(3-hydroxybutyrate) Pseudomonas luteola M13-4 ?
-
 ?
p-nitrophenyl butyrate + H2O 1.36% of the activity with p-nitrophenydecanoate Pseudomonas luteola 4-nitrophenol + butyrate
-
 ?
p-nitrophenyl decanoate + H2O
-
 Pseudomonas luteola 4-nitrophenol + decanoate
-
 ?
p-nitrophenyl decanoate + H2O
-
 Pseudomonas luteola M13-4 4-nitrophenol + decanoate
-
 ?
PHB-co-PHV (60%) + H2O 74% of activity with poly(3-hydroxyoctanoate) Pseudomonas luteola ?
-
 ?
poly(3-hydroxyheptanoate) + H2O 28.7% of activity with poly(3-hydroxyoctanoate) Pseudomonas luteola ?
-
 ?
poly(3-hydroxynonanoate) + H2O 13.4% of activity with poly(3-hydroxyoctanoate) Pseudomonas luteola ?
-
 ?
poly(3-hydroxyoctanoate) + H2O
-
 Pseudomonas luteola ?
-
 ?
poly(3-hydroxyphenylvalerate) + H2O 4.1% of activity with poly(3-hydroxyoctanoate) Pseudomonas luteola ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 28000, SDS-PAGE Pseudomonas luteola

Synonyms

Synonyms Comment Organism
(PHO) depolymerase
-
 Pseudomonas luteola

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Pseudomonas luteola

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 50 25°C: about 65% of maximal activity, 50°C: about 70% of maximal activity Pseudomonas luteola

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
 Pseudomonas luteola

pH Range

pH Minimum pH Maximum Comment Organism
8.5 10.5 pH 8.5: about 40% of maximal activity, pH 10.5: about 90% of maximal activity Pseudomonas luteola

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas luteola isoelectric focusing
-
 6