Literature summary for 3.1.1.76 extracted from

Raiger-Iustman, L.J.; Ruiz, J.A.
The alternative sigma factor, sigmaS, affects polyhydroxyalkanoate metabolism in Pseudomonas putida (2008), FEMS Microbiol. Lett., 284, 218-224.

Cloned(Commentary)

Cloned (Comment)	Organism
a stationary sigma factor (rpoS)negative mutant is constructed to evaluate polyhydroxyalkanoate accumulation and expression of a translational fusion to the promoter region of the genes that code for polyhydroxyalkanoate synthase 1 (phaC1) and polyhydroxyalkanoate depolymerase (phaZ). The rpoS gene of Pseudomaonas putida KT2440 and its promoter region are amplified with primers and cloned into pBBR1MCS-5 to obtain pBBRrpoS. This plasmid is transferred to Pseudomonas putida SDM75 and SDMPC1.	Pseudomonas putida

Cloned (Comment)

Organism

a stationary sigma factor (rpoS)negative mutant is constructed to evaluate polyhydroxyalkanoate accumulation and expression of a translational fusion to the promoter region of the genes that code for polyhydroxyalkanoate synthase 1 (phaC1) and polyhydroxyalkanoate depolymerase (phaZ). The rpoS gene of Pseudomaonas putida KT2440 and its promoter region are amplified with primers and cloned into pBBR1MCS-5 to obtain pBBRrpoS. This plasmid is transferred to Pseudomonas putida SDM75 and SDMPC1.

Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
additional information	Pseudomonas putida	Enhanced depolymerization obtained in the mutant could be attributable to the higher depolymerase gene (phaZ) expression and the fact that during the stationary phase the carbon source for polyhydroxyalkanoate synthesis is depleted.	?	-	?
additional information	Pseudomonas putida	The genes phaC1 and phaC2, which encode two polyhydroxyalkanoate polymerases, are separated by phaZ, which encodes a polyhydroxyalkanoate depolymerase.	?	-	?
additional information	Pseudomonas putida	the stationary sigma factor might control the genes involved in polyhydroxyalkanoate metabolism. The inactivation of the rpoS gene increases the polyhydroxyalkanoate degradation rate.	?	-	?
additional information	Pseudomonas putida KT 2240	Enhanced depolymerization obtained in the mutant could be attributable to the higher depolymerase gene (phaZ) expression and the fact that during the stationary phase the carbon source for polyhydroxyalkanoate synthesis is depleted.	?	-	?
additional information	Pseudomonas putida KT 2240	The genes phaC1 and phaC2, which encode two polyhydroxyalkanoate polymerases, are separated by phaZ, which encodes a polyhydroxyalkanoate depolymerase.	?	-	?
additional information	Pseudomonas putida KT 2240	the stationary sigma factor might control the genes involved in polyhydroxyalkanoate metabolism. The inactivation of the rpoS gene increases the polyhydroxyalkanoate degradation rate.	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	-	-	-
Pseudomonas putida KT 2240	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
additional information	Enhanced depolymerization obtained in the mutant could be attributable to the higher depolymerase gene (phaZ) expression and the fact that during the stationary phase the carbon source for polyhydroxyalkanoate synthesis is depleted.	Pseudomonas putida	?	-	?
additional information	The genes phaC1 and phaC2, which encode two polyhydroxyalkanoate polymerases, are separated by phaZ, which encodes a polyhydroxyalkanoate depolymerase.	Pseudomonas putida	?	-	?
additional information	the stationary sigma factor might control the genes involved in polyhydroxyalkanoate metabolism. The inactivation of the rpoS gene increases the polyhydroxyalkanoate degradation rate.	Pseudomonas putida	?	-	?
additional information	Enhanced depolymerization obtained in the mutant could be attributable to the higher depolymerase gene (phaZ) expression and the fact that during the stationary phase the carbon source for polyhydroxyalkanoate synthesis is depleted.	Pseudomonas putida KT 2240	?	-	?
additional information	The genes phaC1 and phaC2, which encode two polyhydroxyalkanoate polymerases, are separated by phaZ, which encodes a polyhydroxyalkanoate depolymerase.	Pseudomonas putida KT 2240	?	-	?
additional information	the stationary sigma factor might control the genes involved in polyhydroxyalkanoate metabolism. The inactivation of the rpoS gene increases the polyhydroxyalkanoate degradation rate.	Pseudomonas putida KT 2240	?	-	?

Synonyms

Synonyms	Comment	Organism
PhaZ	-	Pseudomonas putida
polyhydroxyalkanoate depolymerase	-	Pseudomonas putida