Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.76 extracted from

  • Heinrich, D.; Raberg, M.; Fricke, P.; Kenny, S.T.; Morales-Gamez, L.; Babu, R.P.; OConnor, K.E.; Steinbuechel, A.
    Synthesis gas (Syngas)-derived medium-chain-length polyhydroxyalkanoate synthesis in engineered Rhodospirillum rubrum (2016), Appl. Environ. Microbiol., 82, 6132-6140 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis engineering of Rhodospirillum rubrum to synthesize a heteropolymer of mainly 3-hydroxydecanoic acid and 3-hydroxyoctanoic acid from CO- and CO2-containing artificial synthesis gas (syngas) using Pseudomonas putida 3-hydroxyacyl acyl carrier protein thioesterase, a medium-chain-length fatty acid coenzyme A ligase, and an medium-chain-length polyhydroxyalkanoate synthase. A heteropolymer of 3-hydroxydecanoic acid and 3-hydroxyoctanoic acid accumulates to up to 7.1% (wt/wt) of the cell dry weight. The recombinant strains are able to partially degrade the polymer, and the deletion of isoform PhaZ2 does not reduce mobilization of the accumulated polymer significantly. Mutation C176A in the active site of PhaZ2 leads to a slight increase in poly-3-hydroxyalkanoate accumulation Rhodospirillum rubrum

Protein Variants

Protein Variants Comment Organism
C176A mutation C176A in the active site leads to a slight increase in poly-3-hydroxyalkanoate accumulation in a recombinant strain Rhodospirillum rubrum

Organism

Organism UniProt Comment Textmining
Rhodospirillum rubrum Q2RNZ5
-
-
Rhodospirillum rubrum DSM 467 Q2RNZ5
-
-

Synonyms

Synonyms Comment Organism
Rru_A3356
-
Rhodospirillum rubrum