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Literature summary for 3.1.1.76 extracted from
- Novel extracellular medium-chain-length polyhydroxyalkanoate depolymerase from Streptomyces exfoliatus K10 DSMZ 41693 a promising biocatalyst for the efficient degradation of natural and functionalized mcl-PHAs (2015), Appl. Microbiol. Biotechnol., 99, 9605-9615 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| SDS | 0.01%, 166.7% of initial activity | Streptomyces exfoliatus |  |
| Triton X-100 | 0.005%, 300% of initial activity, inhibitory at 0.01% | Streptomyces exfoliatus | |
| Tween 20 | 0.01%, 227.9% of initial activity | Streptomyces exfoliatus | |
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | enzyme is able to degrade functionalized polyhydroxyalkanoate polymers containing thioester groups in the side chain, releasing functional thioester-based monomers and oligomers | Streptomyces exfoliatus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Rhodococcus sp. | Streptomyces exfoliatus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 2 mM, complete inactivation | Streptomyces exfoliatus | |
| EDTA | 10 mM, complete loss of activity | Streptomyces exfoliatus | |
| Mn2+ | 2 mM, complete inactivation | Streptomyces exfoliatus | |
| Triton X-100 | 0.01%, 80% of initial activity, activating at 0.05% | Streptomyces exfoliatus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | recombinant enzyme | Streptomyces exfoliatus | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces exfoliatus | - |
- |
- |
| Streptomyces exfoliatus DSMZ 41693 | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 42 | - |
pH 10, 30°C | Streptomyces exfoliatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polyhydroxyoctanoate-co-hexanoate + H2O | - |
Streptomyces exfoliatus | (R)-3-hydroxyoctanoic acid + ? | - |
? | |
| polyhydroxyoctanoate-co-hexanoate + H2O | - |
Streptomyces exfoliatus DSMZ 41693 | (R)-3-hydroxyoctanoic acid + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 27600, calculated from sequence, x * 30000, SDS-PAGE | Streptomyces exfoliatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Streptomyces exfoliatus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
56% residual activity | Streptomyces exfoliatus |
| 45 | - |
15% residual activity | Streptomyces exfoliatus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
thermal stability is improved by 10% glycerol | Streptomyces exfoliatus |
| 44.2 | - |
melting temperature | Streptomyces exfoliatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
- |
Streptomyces exfoliatus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
30% residual activity | Streptomyces exfoliatus |
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Release 2023.1 (January 2023)
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