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Literature summary for 3.1.1.76 extracted from
- Establishment of a biosynthesis pathway for (R)-3-hydroxyalkanoates in recombinant Escherichia coli (2015), Korean J. Chem. Engin., 32, 702-706 .
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | construcution of a biosynthetic pathway for the production of (R)-3-hydroxyalkanoates through in vivo depolymerization of poly(3-hydroxyalkanoates) in an Escherichia coli fadA mutant WA101 by introducing the Pseudomonas sp. 61-3 polyhydroxyalkanoate synthase gene (phaC2) and the Pseudomonas aeruginosa intracellular polyhydroxyalkanoate depolymerase gene (phaZ). Upon culture in Luria-Bertani (LB) medium containing 2 g/l of sodium decanoate, 3-hydroxyalkanoats can be produced to the concentration of 0.49 g/l. The mole fractions are 7.5 mol% of 3-hydroxybutanoate, 31.6 mol% of 3-hydroxyhexanoate, 30 mol% of 3-hydroxyoctanoate, 29.4 mol% of 3-hydroxydecanoate, and 1.5 mol% of 3-hydroxydodecanoate | Pseudomonas aeruginosa |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PhaZ | - |
Pseudomonas aeruginosa |
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Release 2023.1 (January 2023)
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