Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cyclic AMP-dependent protein kinase | phosphorylation, 40% activation of triolein hydrolyzing activity | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diisopropylfluorophosphate | 0.012 mM, 50% inhibition after 30 min incubation | Bos taurus | |
Emulgen 120 | 0.1%, 955 of cholesteryl oleate hydrolysis | Bos taurus | |
NaF | 20 mM, 50% inhibition of p-nitrophenyl butyrate hydrolysis, 70% of triolein hydrolysis, 60% of cholesteryl hydrolysis | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.164 | - |
p-nitrophenyl butyrate | 37°C, pH 6.8 | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | - |
Bos taurus |
Purification (Comment) | Organism |
---|---|
pH 5.0, DE-52, Phenyl-Sepharose, Mono Q, Mono S | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.8 | - |
hydrolysis of p-nitrophenyl laurate | Bos taurus |
0.9 | - |
hydrolysis of p-nitrophenyl palmitate | Bos taurus |
3.8 | - |
hydrolysis of cholesteryl oleate | Bos taurus |
4.7 | - |
hydrolysis of p-nitrophenyl acetate | Bos taurus |
22 | - |
hydrolysis of p-nitrophenyl butyrate | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesteryl oleate + H2O | - |
Bos taurus | cholesterol + oleate | - |
? | |
diacylglycerol + H2O | - |
Bos taurus | monoacylglycerol + a carboxylate | - |
? | |
monoacylglycerol + H2O | - |
Bos taurus | glycerol + a carboxylate | - |
? | |
p-nitrophenyl acetate + H2O | - |
Bos taurus | p-nitrophenol + acetate | - |
? | |
p-nitrophenyl butyrate + H2O | - |
Bos taurus | p-nitrophenol + butanoate | - |
? | |
p-nitrophenyl laurate + H2O | - |
Bos taurus | p-nitrophenol + laurate | - |
? | |
p-nitrophenyl palmitate + H2O | - |
Bos taurus | p-nitrophenol + palmitate | - |
? | |
triacylglycerol + H2O | - |
Bos taurus | diacylglycerol + a carboxylate | - |
? | |
triolein + H2O | - |
Bos taurus | dioleoylglycerol + oleate | - |
? |