Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AgNO3 | - |
Pseudomonas aeruginosa | |
atropine | competitive | Pseudomonas aeruginosa | |
CuSO4 | - |
Pseudomonas aeruginosa | |
diisopropylphosphofluoridate | irreversible inhibition | Pseudomonas aeruginosa | |
HgCl2 | - |
Pseudomonas aeruginosa | |
homatropine | competitive | Pseudomonas aeruginosa | |
iodoacetic acid | - |
Pseudomonas aeruginosa | |
additional information | enzyme is inhibited by quaternary ammonium salts. The effect is influenced by the size of the quaternary ammonium ion | Pseudomonas aeruginosa | |
p-chloromercuribenzoate | - |
Pseudomonas aeruginosa | |
physostigmine | i.e. eserine; irreversible inhibition | Pseudomonas aeruginosa | |
procaine | competitive | Pseudomonas aeruginosa | |
quinidine | competitive | Pseudomonas aeruginosa | |
Scopolamine | competitive | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
Butyrylcholine | pH 7.5, 25°C | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
gel filtration | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
IFO 3918 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas aeruginosa |
Storage Stability | Organism |
---|---|
-20°C, 0.1 M potassium phosphate buffer, pH 7.4, 1 month, no loss of activity | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoylcholine + H2O | - |
Pseudomonas aeruginosa | benzoate + choline | - |
? | |
butyrylcholine + H2O | best substrate | Pseudomonas aeruginosa | butanoate + choline | - |
? | |
butyrylthiocholine + H2O | good substrate | Pseudomonas aeruginosa | butanoate + thiocholine | - |
? | |
ethyl n-butyrate + H2O | poor substrate | Pseudomonas aeruginosa | ethanol + n-butanoate | - |
? | |
isoamyl isovalerate + H2O | poor substrate | Pseudomonas aeruginosa | isopentanol + isopentanoate | - |
? | |
isoamyl n-butyrate + H2O | - |
Pseudomonas aeruginosa | isopentanol + n-butanoate | - |
? | |
isoamyl propionate + H2O | - |
Pseudomonas aeruginosa | isopentanol + propanoate | - |
? | |
isobutyl isovalerate + H2o | poor substrate | Pseudomonas aeruginosa | isobutanol + isopentanoate | - |
? | |
isobutyl n-butyrate + H2O | - |
Pseudomonas aeruginosa | isobutanol + n-butanoate | - |
? | |
isobutyl propionate + H2O | poor substrate | Pseudomonas aeruginosa | isobutanol + propanoate | - |
? | |
methyl n-butyrate + H2O | poor substrate | Pseudomonas aeruginosa | methanol + n-butanoate | - |
? | |
methyl n-valerate + H2O | poor substrate | Pseudomonas aeruginosa | methanol + n-pentanoate | - |
? | |
additional information | butyryl esters are hydrolyzed at a greater rate than propionyl, pentanoyl, valeryl- or acetyl esters | Pseudomonas aeruginosa | ? | - |
? | |
n-amyl acetate + H2O | poor substrate | Pseudomonas aeruginosa | n-pentanol + acetate | - |
? | |
n-amyl n-butyrate + H2O | good substrate | Pseudomonas aeruginosa | n-pentanol + n-butanoate | - |
? | |
n-butyl acetate + H2O | poor substrate | Pseudomonas aeruginosa | n-butanol + acetate | - |
? | |
n-butyl n-butyrate + H2O | poor substrate | Pseudomonas aeruginosa | n-butanol + n-butanoate | - |
? | |
n-hexyl acetate + H2O | poor substrate | Pseudomonas aeruginosa | n-hexanol + acetate | - |
? | |
propionylcholine + H2O | good substrate | Pseudomonas aeruginosa | propanoate + choline | - |
? | |
propionylthiocholine + H2O | - |
Pseudomonas aeruginosa | propanoate + thiocholine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
butyrylcholine-hydrolyzing enzyme | - |
Pseudomonas aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
0.1 M phosphate buffer, pH 5.5, 4% loss of activity | Pseudomonas aeruginosa |
80 | - |
above 80°C, 10 min, 0.1 M phosphate buffer, pH 5.5, complete inactivation | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | optimum reactivity for hydrolysis of butyrylcholine as substrate | Pseudomonas aeruginosa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
5 min, 75°C, 90% loss of activity | Pseudomonas aeruginosa |
5.5 | 7 | 5 min, 75°C, stable between | Pseudomonas aeruginosa |
8.5 | - |
5 min, 75°C, 60% loss of activity | Pseudomonas aeruginosa |