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Literature summary for 3.1.1.82 extracted from
- Two enzymatic reaction pathways in the formation of pyropheophorbide a (2002), Photosynth. Res., 74, 225-233.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 1 mM, 22% activation, Phedase type 1 | Raphanus sativus |  |
| EDTA | 1 mM, 9% activation, Phedase type 2 | Raphanus sativus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| methanol | 1 M, 20% inhibition, Phedase type 1; 1 M, 20% inhibition, Phedase type 2 | Raphanus sativus | |
| N-ethylmaleimide | 1 mM, 11% inhibition, Phedase type 1 | Raphanus sativus | |
| phenylmethylsulfonic fluoride | 1 mM, 26.4% inhibition, Phedase type 2; 1 mM, 61% inhibition, Phedase type 1 | Raphanus sativus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0141 | - |
pheophorbide a | 30°C, pH 7.0, Phedase type 1 | Raphanus sativus | |
| 0.0151 | - |
pheophorbide a | 30°C, pH 7.0, Phedase type 2 | Raphanus sativus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
2 * 57000, Phedase type 1, SDS-PAGE | Raphanus sativus |
| 113000 | - |
Phedase type 1, gel filtration | Raphanus sativus |
| 113000 | - |
Phedase type 2, gel filtration | Raphanus sativus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pheophorbide a + H2O | Raphanus sativus | Phedase type 1 is senescence-induced | pyropheophorbide a + methanol + CO2 | - |
? | |
| pheophorbide a + H2O | Raphanus sativus | Phedase type 2 is constitutively produced | pyropheophorbide a + methanol + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
low activity | - |
| Brassica napus | - |
- |
- |
| Brassica oleracea | - |
- |
- |
| Brassica rapa subsp. chinensis | - |
- |
- |
| Brassica rapa subsp. oleifera | - |
- |
- |
| Brassica rapa subsp. pekinensis | - |
low activity | - |
| Chenopodium album | - |
- |
- |
| Hypolepis punctata | - |
- |
- |
| Nasturtium officinale | - |
- |
- |
| no activity in Camellia sinensis | - |
- |
- |
| no activity in Chlamydomonas reinhardtii | - |
- |
- |
| no activity in Chrysanthemum coronarium | - |
- |
- |
| no activity in Corchorus olitorius | - |
- |
- |
| no activity in Equisetum arvense | - |
- |
- |
| no activity in Ginkgo biloba | - |
- |
- |
| no activity in Hordeum vulgare | - |
- |
- |
| no activity in Lepisorus thunbergianus | - |
- |
- |
| no activity in Perilla frutescens | - |
- |
- |
| no activity in Petroselinum sativum | - |
- |
- |
| no activity in Pisum sativum | - |
- |
- |
| no activity in Rumex acetosa | - |
- |
- |
| no activity in Spinacia oleracea | - |
- |
- |
| Raphanus sativus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Phedase type 1 | Raphanus sativus |
| Phedase type 2 | Raphanus sativus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | - |
Raphanus sativus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pheophorbide a + H2O | Phedase type 1 is senescence-induced | Raphanus sativus | pyropheophorbide a + methanol + CO2 | - |
? | |
| pheophorbide a + H2O | Phedase type 2 is constitutively produced | Raphanus sativus | pyropheophorbide a + methanol + CO2 | - |
? | |
| pheophorbide a + H2O | the enzyme catalyzes the conversion of pheophorbide a to a precursor of pyropheophorbide a, C-132-carboxylpyropheophorbide a, by demethylation, and then the precursor is decarboxylated non-enzymatically to yield pyropheophorbide a, Phedase type 1 | Raphanus sativus | pyropheophorbide a + methanol + CO2 | - |
? | |
| pheophorbide a + H2O | the enzyme catalyzes the conversion of pheophorbide a to a precursor of pyropheophorbide a, C-132-carboxylpyropheophorbide a, by demethylation, and then the precursor is decarboxylated non-enzymatically to yield pyropheophorbide a, Phedase type 2 | Raphanus sativus | pyropheophorbide a + methanol + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Raphanus sativus |
| dimer | 2 * 57000, Phedase type 1, SDS-PAGE | Raphanus sativus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| phedase | - |
Arabidopsis thaliana |
| phedase | - |
Brassica napus |
| phedase | - |
Raphanus sativus |
| phedase | - |
Brassica oleracea |
| phedase | - |
Brassica rapa subsp. oleifera |
| phedase | - |
Brassica rapa subsp. pekinensis |
| phedase | - |
Nasturtium officinale |
| phedase | - |
Chenopodium album |
| phedase | - |
Brassica rapa subsp. chinensis |
| phedase | - |
Hypolepis punctata |
| Phedase type 1 | - |
Raphanus sativus |
| Phedase type 2 | - |
Raphanus sativus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 68 | - |
50% loss of activity at 67.5°C, Phedase type 1 | Raphanus sativus |
| 68 | - |
50% loss of activity at 67.5°C, Phedase type 2 | Raphanus sativus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
Phedase type 2 | Raphanus sativus |
| 7 | 7.5 | Phedase type 1 | Raphanus sativus |
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