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Literature summary for 3.1.1.83 extracted from
- Genetic and biochemical characterization of a novel monoterpene epsilon-lactone hydrolase from Rhodococcus erythropolis DCL14 (2001), Appl. Environ. Microbiol., 67, 733-741.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Rhodococcus erythropolis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,2'-dipyridyl | 1 mM, 55% inhibition | Rhodococcus erythropolis |  |
| 2-Bromo-4'-nitroacetophenone | 1 mM, 84% inhibition | Rhodococcus erythropolis | |
| CaCl2 | 1 mM, 27% inhibition | Rhodococcus erythropolis | |
| CoCl2 | 1 mM, 71% inhibition | Rhodococcus erythropolis | |
| EDTA | 10 mM, 16% inhibition | Rhodococcus erythropolis | |
| HgCl2 | 0.1 mM 95% inhibition | Rhodococcus erythropolis | |
| MnCl2 | 1 mM, 37% inhibition | Rhodococcus erythropolis | |
| p-chloromercuribenzoate | 0.1 mM, 26% inhibition | Rhodococcus erythropolis | |
| phenylhydrazine | 1 mM, 17% inhibition | Rhodococcus erythropolis | |
| SDS | 1 mM, 78% inhibition | Rhodococcus erythropolis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
gel filtration | Rhodococcus erythropolis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Rhodococcus erythropolis | DCL14, catalyzing the ring opening of lactones which are formed during degradation of several monocyclic monoterpenes, including carvone and menthol | ? | - |
? | |
| additional information | Rhodococcus erythropolis DCL14 | DCL14, catalyzing the ring opening of lactones which are formed during degradation of several monocyclic monoterpenes, including carvone and menthol | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus erythropolis | - |
- |
- |
| Rhodococcus erythropolis DCL14 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rhodococcus erythropolis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:(4R)-limonene-grown cell | - |
Rhodococcus erythropolis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 145 | - |
- |
Rhodococcus erythropolis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, MLH can be stored for 6 months, without loss of activity | Rhodococcus erythropolis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (4R)-4-isopropenyl-7-methyl-2-oxo-oxepanone + H2O | - |
Rhodococcus erythropolis | 6-hydroxy-3-(1-methylethenyl)heptanoic acid | - |
? | |
| (4R)-4-isopropenyl-7-methyl-2-oxo-oxepanone + H2O | - |
Rhodococcus erythropolis DCL14 | 6-hydroxy-3-(1-methylethenyl)heptanoic acid | - |
? | |
| (6R)-6-isopropenyl-3-methyl-2-oxo-oxepanone + H2O | - |
Rhodococcus erythropolis | 5-(hydroxymethyl)-2,6-dimethylhept-6-enoic acid | - |
? | |
| (6R)-6-isopropenyl-3-methyl-2-oxo-oxepanone + H2O | - |
Rhodococcus erythropolis DCL14 | 5-(hydroxymethyl)-2,6-dimethylhept-6-enoic acid | - |
? | |
| epsilon-caprolactone + H2O | - |
Rhodococcus erythropolis | ? | - |
? | |
| epsilon-caprolactone + H2O | - |
Rhodococcus erythropolis DCL14 | ? | - |
? | |
| ethyl caproate + H2O | 23% of the activity with epsilon-caprolactone | Rhodococcus erythropolis | ? | - |
? | |
| ethyl caproate + H2O | 23% of the activity with epsilon-caprolactone | Rhodococcus erythropolis DCL14 | ? | - |
? | |
| additional information | DCL14, catalyzing the ring opening of lactones which are formed during degradation of several monocyclic monoterpenes, including carvone and menthol | Rhodococcus erythropolis | ? | - |
? | |
| additional information | DCL14, catalyzing the ring opening of lactones which are formed during degradation of several monocyclic monoterpenes, including carvone and menthol | Rhodococcus erythropolis DCL14 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 35000, gel filtration | Rhodococcus erythropolis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| mlhB | - |
Rhodococcus erythropolis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Rhodococcus erythropolis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 13 | 37 | 13°C: about 35% of maximal activity, 37°C: 75% of maximal activity | Rhodococcus erythropolis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
inactivation above | Rhodococcus erythropolis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Rhodococcus erythropolis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 10.5 | pH 7.5: about 60% of maximal activity, pH 10.5: about 50% of maximal activity | Rhodococcus erythropolis |
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