Cloned (Comment) | Organism |
---|---|
expressed in Sf9 insect cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | the recombinant enzyme is completely inhibited at 1 mM | Homo sapiens | |
AgNO3 | the recombinant enzyme is completely inhibited at 0.1 mM | Homo sapiens | |
bis-4-nitrophenylphosphate | the recombinant enzyme shows about 20% residual activity at 1 mM | Homo sapiens | |
CdCl2 | the recombinant enzyme shows about 20% residual activity at 1 mM | Homo sapiens | |
CuCl2 | the recombinant enzyme shows about 20% residual activity at 1 mM | Homo sapiens | |
diisopropyl fluorophosphate | the recombinant enzyme shows about 63% residual activity at 1 mM | Homo sapiens | |
Disulfiram | the recombinant enzyme shows about 23% residual activity at 1 mM | Homo sapiens | |
eserine | the recombinant enzyme shows about 85% residual activity at 1 mM | Homo sapiens | |
additional information | the recombinant enzyme is not inhibited by CoCl2, CaCl2, D-saccharic acid 1,4-lactone, and sodium fluoride | Homo sapiens | |
N-ethylmaleimide | the recombinant enzyme shows about 75% residual activity at 1 mM | Homo sapiens | |
phenylmethylsulfonyl fluoride | the recombinant enzyme shows about 25% residual activity at 1 mM | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1007 | - |
mycophenolic acid O-acyl-glucuronide | recombinant enzyme, in 100 mM Tris-HCl (pH 7.4), at 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
microsome | - |
Homo sapiens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
x * 28000, precursor protein, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9NUJ1 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, CM Sepharose column chromatography, Mono P column chromatography, and Superdex 200 gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00017 | - |
unpurified enzyme from cytosol, in 100 mM Tris-HCl (pH 7.4), at 37°C | Homo sapiens |
0.00954 | - |
after 55.1fold purification, in 100 mM Tris-HCl (pH 7.4), at 37°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
mycophenolic acid O-acyl-glucuronide + H2O | - |
Homo sapiens | mycophenolic acid + D-glucuronate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28000, precursor protein, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
ABHD10 | - |
Homo sapiens |