Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Na+ | substrate: artificial polynucleotides | Escherichia coli | |
Poly(U) | strong competitive inhibitor of T4 mRNA-degradation | Escherichia coli | |
rRNA | weak inhibitor of T4 mRNA-degradation | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | activation against poly(A), -(U), -(C), RNA and T4 mRNA | Escherichia coli | |
Li+ | can substitute for K+ to a small extend | Escherichia coli | |
Na+ | increases activity against T4 mRNA, can substitute for K+ in activation against T4 mRNA and RNA | Escherichia coli | |
NH4+ | activating | Escherichia coli | |
NH4+ | can substitute for K+ to a small extend | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | 90000 | native and SDS-PAGE, gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ss RNA + H2O | Escherichia coli | - |
5'-phosphomononucleotides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to apparent homogeneity, 270fold | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.04 | - |
activity of the most active fraction, substrate: T4 mRNA | Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, small aliquots of purified enzyme, 10% glycerol, stable for at least 19 months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ss RNA + H2O | - |
Escherichia coli | 5'-phosphomononucleotides | - |
? | |
T4 mRNA + H2O | - |
Escherichia coli | 5'-phosphomononucleotides | - |
? |