Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.13.1 extracted from

  • Sporn, M.B.; Lazarus, H.M.; Smith, J.M.; Henderson, W.R.
    Studies on nuclear exoribonucleases. 3. Isolation and properties of the enzyme from normal and malignant tissues of the mouse (1969), Biochemistry, 8, 1698-1706.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
RNA with a 2',3'-cyclic phosphate group on the potential substrate molecule Mus musculus
RNA with a terminal 3'-phosphate group on the potential substrate molecule Mus musculus
thymidine nucleotides potent inhibitor, pTp at a concentration of 0.00004 M inhibits degradation of poly(A) by 60%, pTpT at a concentration of 0.0005 M inhibits degradation of poly(A) by 30% Mus musculus
Urea loss of 40% of activity in presence of 1.6 M, 60% in presence of 2.4 M and 75% in presence of 3.2 M urea Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
Mus musculus 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ss RNA + H2O Mus musculus
-
5'-phosphomononucleotides
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
mouse
-

Reaction

Reaction Comment Organism Reaction ID
Exonucleolytic cleavage in the 3'- to 5'- direction to yield nucleoside 5'-phosphates acts in a processive manner Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
breast adenocarcinoma cell
-
Mus musculus
-
Ehrlich ascites carcinoma cell
-
Mus musculus
-
embryo
-
Mus musculus
-
kidney
-
Mus musculus
-
liver
-
Mus musculus
-

Storage Stability

Storage Stability Organism
-70°C, more than 50% of activity after more than a year Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information 3'-phosphorylated RNA is not hydrolyzed Mus musculus ?
-
?
poly(A) + H2O
-
Mus musculus 5'-AMP + oligonucleotide
-
?
ss RNA + H2O
-
Mus musculus 5'-phosphomononucleotides
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
in 0.1 M Tris-Cl, pH 8.0, for 15 min, loss of 20-30% of activity Mus musculus
50
-
in 0.1 M Tris-Cl, pH 8.0, for 60 min, loss of 40-60% of activity Mus musculus