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Literature summary for 3.1.13.1 extracted from
- A single mutation in Escherichia coli ribonuclease II inactivates the enzyme without affecting RNA binding (2005), FEBS J., 272, 363-374.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| IPTG | 1 mM, after 2 h RNase major protein in cell extracts of E.coli strain BL21(DE3) | Escherichia coli |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Escherichia coli strain JM109 | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | metal binding | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | for catalysis of mRNA but not for substrate binding | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
and BL21(DE3) | - |
| Escherichia coli JM109 | - |
and BL21(DE3) | - |
| no activity in Escherichia coli | - |
carrying the mutant allele rnb296, single substitution of aspartate 209 for asparagine | - |
| no activity in Escherichia coli SK4803 | - |
carrying the mutant allele rnb296, single substitution of aspartate 209 for asparagine | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Escherichia coli strain BL21(DE3) | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| m-RNA + H2O | SL9A, containing one stem-loop structure, and malE-malF operon, containing two stem-loop structures | Escherichia coli | ? | - |
? | |
| m-RNA + H2O | SL9A, containing one stem-loop structure, and malE-malF operon, containing two stem-loop structures | Escherichia coli JM109 | ? | - |
? | |
| poly[8-3H]adenylic acid + H2O | linear substrate, activity below 325 UE·micro g-1 | Escherichia coli | ? | - |
? | |
| poly[8-3H]adenylic acid + H2O | linear substrate, activity below 325 UE·micro g-1 | Escherichia coli JM109 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ribonuclease II | - |
Escherichia coli |
| RNase II | - |
Escherichia coli |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10 | - |
EDTA | malE-malF mRNA transcripts incubated at 37°C | Escherichia coli | |
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