Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.13.1 extracted from

  • Schneider, C.; Anderson, J.T.; Tollervey, D.
    The exosome subunit Rrp44 plays a direct role in RNA substrate recognition (2007), Mol. Cell, 27, 324-331.
    View publication on PubMed
Search for more literature for 3.1.13.1

Cloned(Commentary)

Cloned (Comment) Organism
wild-type Rrp44, Rrp44-20, and Rrp44-cat are expressed in Escherichia coli as GST fusions Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
D551N mutation in Rrp44-cat abolishes the exonucleolytic activity of Rrp44 without affecting its ability to bind RNA Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tRNAiMet + H2O Saccharomyces cerevisiae complete degradation of the hypomodified tRNA requires both Rrp44 and the poly(A) polymerase activity of TRAMP. The intact exosome lacking only the catalytic activity of Rrp44 fails to degrade tRNAi Met, showing this to be a specific Rrp44 substrate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P39112 mitochondrial precursor
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5' end-labeled pre-tRNASer + H2O degradation of pre-tRNASer in a processive manner, leaving a short oligonucleotide (about 3nt) product Saccharomyces cerevisiae ?
-
 ?
tRNAiMet + H2O complete degradation of the hypomodified tRNA requires both Rrp44 and the poly(A) polymerase activity of TRAMP. The intact exosome lacking only the catalytic activity of Rrp44 fails to degrade tRNAi Met, showing this to be a specific Rrp44 substrate Saccharomyces cerevisiae ?
-
 ?

Synonyms

Synonyms Comment Organism
Rrp44
-
 Saccharomyces cerevisiae