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Literature summary for 3.1.13.5 extracted from
- Escherichia coli RNase D. Purification and structural characterization of a putative processing nuclease (1981), J. Biol. Chem., 256, 5627-5632.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoic acid) | the enzyme is relatively insensitive to sulfhydryl reagents | Escherichia coli |  |
| HgCl2 | the enzyme is relatively insensitive to sulfhydryl reagents, increased inhibition after addition of dithiothreitol | Escherichia coli | |
| NEM | the enzyme is relatively insensitive to sulfhydryl reagents | Escherichia coli | |
| p-hydroxymercuribenzoate | the enzyme is relatively insensitive to sulfhydryl reagents | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | the enzyme is devoid of nucleic acid | Escherichia coli |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 6 months, enzyme retains 90% of its activity | Escherichia coli |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 40000, SDS-PAGE | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the enzyme is sensitive to inactivation by elevated temperatures but can be protected by a variety of RNAs | Escherichia coli |
| 50 | - |
3-4 min, 50% loss of the activity against phosphodiesterase-treated tRNA | Escherichia coli |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Escherichia coli | electrofocusing, pH-range 2-11 | - |
6.2 |
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