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Literature summary for 3.1.2.12 extracted from
- Physiological role of S-formylglutathione hydrolase in C(1) metabolism of the methylotrophic yeast Candida boidinii (2003), Microbiology, 149, 1971-1979.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene fgh1, DNA and amino acid sequence determination and analysis, expression in DH5alpha, expression as GFP-fusion protein in Candida boidinii | [Candida] boidinii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | fgh1 gene disruption impairs the ability to grow on methanol and reduces the growth rate on methylamine and choline as sole nitrogen source, while growth on glucose or glycerol is not affected | [Candida] boidinii |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | detection of recombinant GFP-fusion protein | [Candida] boidinii | 5829 | - |
| peroxisome | detection of recombinant GFP-fusion protein | [Candida] boidinii | 5777 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-formylglutathione + H2O | [Candida] boidinii | enzyme is involved in the glutathione-dependent formaldehyde oxidation pathway during growth on methanol as the sole carbon source, regulation of gene expression | glutathione + formate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| [Candida] boidinii | - |
methylotrophic yeast, gene fgh1, strain S2 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-formylglutathione + H2O | - |
[Candida] boidinii | glutathione + formate | - |
? | |
| S-formylglutathione + H2O | enzyme is involved in the glutathione-dependent formaldehyde oxidation pathway during growth on methanol as the sole carbon source, regulation of gene expression | [Candida] boidinii | glutathione + formate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FGH | - |
[Candida] boidinii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | [Candida] boidinii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at | [Candida] boidinii |
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