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Literature summary for 3.1.2.20 extracted from

  • Cheesbrough, T.M.; Kolattukudy, P.E.
    Purification and characterization of a fatty acyl-CoA hydrolase from the uropygial glands of Peking ducks (Anas domesticus) (1985), Arch. Biochem. Biophys., 237, 208-216.
    View publication on PubMed
Search for more literature for 3.1.2.20

General Stability

General Stability Organism
no dithioerythritol or other mercaptam required to maintain activity Anas platyrhynchos

Inhibitors

Inhibitors Comment Organism Structure
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide 23% inhibition at 10 mM Anas platyrhynchos
diethyldicarbonate 95% inhibition at 2 mM Anas platyrhynchos
diisopropylfluorophosphate 94% inhibition at 5 mM, maximal inhibition Anas platyrhynchos
p-hydroxymercuribenzoate 99% inhibition at 0.1 mM Anas platyrhynchos
PMSF 96% inhibition at 1 mM Anas platyrhynchos

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Anas platyrhynchos 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56300
-
 gel filtration Anas platyrhynchos

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + H2O Anas platyrhynchos
-
 CoA + a carboxylate
-
 ?

Organism

Organism UniProt Comment Textmining
Anas platyrhynchos
-
 4 isoforms of different pI and MW
-
Anas platyrhynchos
-
 Peking or mallard duck
-

Purification (Commentary)

Purification (Comment) Organism
184fold Anas platyrhynchos

Source Tissue

Source Tissue Comment Organism Textmining
uropygial gland
-
 Anas platyrhynchos
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
45.4
-
 partially purified enzyme Anas platyrhynchos

Storage Stability

Storage Stability Organism
-20°C, purified lyophilized, at least 6 months Anas platyrhynchos

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + H2O
-
 Anas platyrhynchos CoA + a carboxylate
-
 ?
acyl-CoA + H2O broad specificity for medium-chain to long-chain acyl-CoA Anas platyrhynchos CoA + a carboxylate
-
 ?
decanoyl-CoA + H2O
-
 Anas platyrhynchos CoA + decanoate
-
 ?
dodecanoyl-CoA + H2O i.e. lauroyl-CoA Anas platyrhynchos CoA + dodecanoate
-
 ?
additional information substrate specificity alters with pH Anas platyrhynchos ?
-
 ?
octanoyl-CoA + H2O
-
 Anas platyrhynchos CoA + octanoate
-
 ?
tetradecanoyl-CoA + H2O i.e. myristoyl-CoA Anas platyrhynchos CoA + tetradecanoate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 55800-59400, 4 isoforms, SDS-PAGE Anas platyrhynchos

Synonyms

Synonyms Comment Organism
More cf. EC 3.1.2.19 Anas platyrhynchos
More immunologically not related with the thioesterase of fatty acid synthase or the acyl fatty acid synthase thioester hydrolase Anas platyrhynchos

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Anas platyrhynchos

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
-
 Anas platyrhynchos
9.3
-
 dodecanoyl-CoA as substrate Anas platyrhynchos

pH Range

pH Minimum pH Maximum Comment Organism
7.6 10 about half-maximal activity at pH 7.6 and pH 10.0, dodecanoyl-CoA as substrate Anas platyrhynchos

pI Value

Organism Comment pI Value Maximum pI Value
Anas platyrhynchos pH 4.4, 4.45, 4.7, 4.9: pIs of isoenzymes
-
 4.4
Anas platyrhynchos pH 4.4, 4.45, 4.7, 4.9: pIs of isoenzymes
-
 4.45
Anas platyrhynchos pH 4.4, 4.45, 4.7, 4.9: pIs of isoenzymes 4.8 4.7
Anas platyrhynchos pH 4.4, 4.45, 4.7, 4.9: pIs of isoenzymes
-
 4.9