Cloned (Comment) | Organism |
---|---|
the gene encoding YciA is amplified by PCR and expressed in Escherichia coli strain BL21 (DE3) | Escherichia coli |
the gene encoding YciA is amplified by PCR and expressed in Escherichia coli strain BL21 (DE3) | Haemophilus influenzae |
General Stability | Organism |
---|---|
YciA is a highly soluble, robust protein | Haemophilus influenzae |
YciA tends to precipitate at high concentrations | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-hydroxyphenacyl-CoA | - |
Escherichia coli | |
4-hydroxyphenacyl-CoA | - |
Haemophilus influenzae | |
CoA | strong feedback inhibitor, product inhibition | Escherichia coli | |
CoA | strong feedback inhibitor, product inhibition | Haemophilus influenzae | |
hexyl-CoA | - |
Escherichia coli | |
hexyl-CoA | - |
Haemophilus influenzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
phenylacetyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
0.0019 | - |
n-octanoyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
0.0021 | - |
tiglyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
0.0025 | - |
myristoyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
0.0035 | - |
n-butyryl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
0.0037 | - |
3-hydroxyphenylacetyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
0.0041 | - |
n-hexanoyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
0.0042 | - |
linoleoyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
0.0045 | - |
n-decanoyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
0.0047 | - |
acetyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
0.0059 | - |
crotonyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
0.006 | - |
propionyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
0.006 | - |
lauroyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
0.0064 | - |
palmitoleoyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
0.0076 | - |
4-hydroxyphenylacetyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
0.0081 | - |
glutaryl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
0.009 | - |
beta-methylcrotonyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
0.01 | - |
stearoyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
0.011 | - |
oleoyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Escherichia coli | |
0.011 | - |
oleoyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
0.013 | - |
isobutyryl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
0.013 | - |
n-decanoyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Escherichia coli | |
0.014 | - |
isovaleryl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
0.015 | - |
isobutyryl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Escherichia coli | |
0.016 | - |
phenylacetyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Escherichia coli | |
0.017 | - |
palmitoyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
0.02 | - |
succinyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
0.02 | - |
lauroyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Escherichia coli | |
0.02 | - |
DL-3-hydroxy-3-methylglutaryl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
0.025 | - |
4-hydroxybenzoyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Escherichia coli | |
0.031 | - |
DL-3-hydroxybutyryl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
0.037 | - |
acetoacetyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
0.039 | - |
arachidoyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
0.068 | - |
n-butyryl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Escherichia coli | |
0.14 | - |
malonyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
0.167 | - |
acetyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Escherichia coli | |
0.17 | - |
methylmalonyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
0.29 | - |
beta-methylcrotonyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Escherichia coli | |
0.8 | - |
n-butyryl-pantetheine phosphate | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Escherichia coli | |
1.4 | - |
n-butyryl-pantetheine phosphate | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
33 | - |
palmitoyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Escherichia coli | 5737 | - |
cytoplasm | - |
Haemophilus influenzae | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15160 | - |
His-tagged YciA protein, determined by electrospray ionization mass spectrometry | Escherichia coli |
16630 | - |
His-tagged YciA protein, determined by electrospray ionization mass spectrometry, N-terminal methionine is removed | Haemophilus influenzae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-Hydroxybenzoyl-CoA + H2O | Escherichia coli | - |
4-Hydroxybenzoate + CoA | - |
? | |
4-Hydroxybenzoyl-CoA + H2O | Haemophilus influenzae | - |
4-Hydroxybenzoate + CoA | - |
? | |
acetyl-CoA + H2O | Escherichia coli | - |
CoA + acetate | - |
? | |
acetyl-CoA + H2O | Haemophilus influenzae | - |
CoA + acetate | - |
? | |
additional information | Escherichia coli | the long, medium, and short chain fatty acyl-CoAs are very good substrates as are the small acyl-CoA metabolites | ? | - |
? | |
additional information | Haemophilus influenzae | the long, medium, and short chain fatty acyl-CoAs are very good substrates as are the small acyl-CoA metabolites | ? | - |
? | |
additional information | Haemophilus influenzae | the substrate activity of propionyl-s-N-acetylcysteine is negligible and that of n-butyryl-pantetheine phosphate low, it is evident YciA does not target acylated holoacyl carrier proteins or other acylated proteins as substrates | ? | - |
? | |
additional information | Escherichia coli | YciA is responsible for the efficient, indiscriminant CoA-regulated hydrolysis of cellular acyl-CoA thioesters in a wide range of bacteria and hypothesize that this activity may support membrane biogenesis | ? | - |
? | |
additional information | Haemophilus influenzae | YciA is responsible for the efficient, indiscriminant CoA-regulated hydrolysis of cellular acyl-CoA thioesters in a wide range of bacteria and hypothesize that this activity may support membrane biogenesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A8Z0 | - |
- |
Haemophilus influenzae | P44886 | - |
- |
Purification (Comment) | Organism |
---|---|
Cells are harvested by centrifugation and suspended in lysis buffer, passed through a French press and centrifuged. The supernatant is loaded onto a Ni-NTA agarose column. The column is washed with a buffer containing 50 mM NaH2PO4, 300 mM NaCl, 50 mM imidazole and 1 mM dithiothreitol, and then the YciA is eluted with an imidazole gradient up to 250 mM imidazole. The pooled and concentrated fractions are chromatographed on a size exclusion column using a buffer containing 50 mM NaH2PO4, 50 mM NaCl, 10 mM imidazole and 1 mM dithiothreitol. | Escherichia coli |
Cells are harvested by centrifugation, and the cell pellet is suspended in HEPES buffer containing 2 mM DTT and 0.2 mM phenylmethanesulfonyl fluoride. The cells are lysed in a French press and the lysate is centrifuged. The supernatant is loaded onto a DEAE Sepharose Fast Flow column equilibrated with HEPES buffer. A linear gradient of KCl in 50 mM Hepes is used to elute the protein. Desired fractions are pooled, concentrated and subjected to ammonium sulfate fractionation. The 40-60% ammonium sulfate fraction is centrifuged, the protein pellet is dissolved, loaded onto a size exclusion column and eluted with 10 mM HEPES containing 0.15 M KCl. | Haemophilus influenzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxyphenylacetyl-CoA + H2O | - |
Haemophilus influenzae | CoA + 3-hydroxyphenylacetate | - |
? | |
4-Hydroxybenzoyl-CoA + H2O | - |
Escherichia coli | 4-Hydroxybenzoate + CoA | - |
? | |
4-Hydroxybenzoyl-CoA + H2O | - |
Haemophilus influenzae | 4-Hydroxybenzoate + CoA | - |
? | |
4-hydroxyphenylacetyl-CoA + H2O | - |
Haemophilus influenzae | CoA + 4-hydroxyphenylacetate | - |
? | |
acetoacetyl-CoA + H2O | - |
Haemophilus influenzae | CoA + acetoacetate | - |
? | |
acetyl-CoA + H2O | - |
Escherichia coli | CoA + acetate | - |
? | |
acetyl-CoA + H2O | - |
Haemophilus influenzae | CoA + acetate | - |
? | |
arachidoyl-CoA + H2O | - |
Haemophilus influenzae | CoA + arachidate | - |
? | |
beta-methylcrotonyl-CoA + H2O | - |
Escherichia coli | CoA + beta-methylcrotonate | - |
? | |
beta-methylcrotonyl-CoA + H2O | - |
Haemophilus influenzae | CoA + beta-methylcrotonate | - |
? | |
crotonyl-CoA + H2O | - |
Haemophilus influenzae | CoA + crotonate | - |
? | |
DL-3-hydroxy-3-methylglutaryl-CoA + H2O | - |
Haemophilus influenzae | CoA + 3-hydroxy-3-methylglutarate | - |
? | |
DL-3-hydroxybutyryl-CoA + H2O | - |
Haemophilus influenzae | CoA + ? | - |
? | |
glutaryl-CoA + H2O | - |
Haemophilus influenzae | CoA + glutarate | - |
? | |
hexanoyl-CoA + H2O | - |
Haemophilus influenzae | CoA + hexanoate | - |
? | |
isobutyryl-CoA + H2O | - |
Escherichia coli | CoA + isobutyrate | - |
? | |
isobutyryl-CoA + H2O | - |
Haemophilus influenzae | CoA + isobutyrate | - |
? | |
isovaleryl-CoA + H2O | - |
Haemophilus influenzae | CoA + isovalerate | - |
? | |
lauroyl-CoA + H2O | - |
Escherichia coli | CoA + laurate | - |
? | |
lauroyl-CoA + H2O | - |
Haemophilus influenzae | CoA + laurate | - |
? | |
linoleoyl-CoA + H2O | - |
Haemophilus influenzae | CoA + linolate | - |
? | |
malonyl-CoA + H2O | - |
Haemophilus influenzae | CoA + malonate | - |
? | |
methylmalonyl-CoA + H2O | - |
Haemophilus influenzae | CoA + methylmalonate | - |
? | |
additional information | the long, medium, and short chain fatty acyl-CoAs are very good substrates as are the small acyl-CoA metabolites | Escherichia coli | ? | - |
? | |
additional information | the long, medium, and short chain fatty acyl-CoAs are very good substrates as are the small acyl-CoA metabolites | Haemophilus influenzae | ? | - |
? | |
additional information | the substrate activity of propionyl-s-N-acetylcysteine is negligible and that of n-butyryl-pantetheine phosphate low, it is evident YciA does not target acylated holoacyl carrier proteins or other acylated proteins as substrates | Haemophilus influenzae | ? | - |
? | |
additional information | YciA is responsible for the efficient, indiscriminant CoA-regulated hydrolysis of cellular acyl-CoA thioesters in a wide range of bacteria and hypothesize that this activity may support membrane biogenesis | Escherichia coli | ? | - |
? | |
additional information | YciA is responsible for the efficient, indiscriminant CoA-regulated hydrolysis of cellular acyl-CoA thioesters in a wide range of bacteria and hypothesize that this activity may support membrane biogenesis | Haemophilus influenzae | ? | - |
? | |
myristoyl-CoA + H2O | - |
Haemophilus influenzae | CoA + myristate | - |
? | |
n-butyryl-CoA + H2O | - |
Escherichia coli | CoA + n-butyrate | - |
? | |
n-butyryl-CoA + H2O | - |
Haemophilus influenzae | CoA + n-butyrate | - |
? | |
n-butyryl-pantetheine phosphate + H2O | - |
Escherichia coli | ? | - |
? | |
n-butyryl-pantetheine phosphate + H2O | - |
Haemophilus influenzae | ? | - |
? | |
n-decanoyl-CoA + H2O | - |
Escherichia coli | CoA + n-decanoate | - |
? | |
n-decanoyl-CoA + H2O | - |
Haemophilus influenzae | CoA + n-decanoate | - |
? | |
n-octanoyl-CoA + H2O | - |
Haemophilus influenzae | CoA + n-octanoate | - |
? | |
oleoyl-CoA + H2O | - |
Escherichia coli | CoA + oleate | - |
? | |
oleoyl-CoA + H2O | - |
Haemophilus influenzae | CoA + oleate | - |
? | |
palmitoleoyl-CoA + H2O | - |
Haemophilus influenzae | CoA + palmitoleoate | - |
? | |
palmitoyl-CoA + H2O | - |
Escherichia coli | CoA + palmitate | - |
? | |
palmitoyl-CoA + H2O | - |
Haemophilus influenzae | CoA + palmitate | - |
? | |
phenylacetyl-CoA + H2O | - |
Escherichia coli | CoA + phenylacetate | - |
? | |
phenylacetyl-CoA + H2O | - |
Haemophilus influenzae | CoA + phenylacetate | - |
? | |
propionyl-CoA + H2O | - |
Haemophilus influenzae | CoA + propionate | - |
? | |
propionyl-s-N-acetylcysteamine + H2O | - |
Haemophilus influenzae | CoA + ? | - |
? | |
stearoyl-CoA + H2O | - |
Haemophilus influenzae | CoA + stearate | - |
? | |
succinyl-CoA + H2O | - |
Haemophilus influenzae | CoA + succinate | - |
? | |
tiglyl-CoA + H2O | - |
Haemophilus influenzae | CoA + (2E)-2-methylbut-2-enoic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | determined by using equilibrium sedimentation techniques | Haemophilus influenzae |
homohexamer | YciA determined by using equilibrium sedimentation techniques | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
acyl-CoA hydrolase | - |
Escherichia coli |
acyl-CoA hydrolase | - |
Haemophilus influenzae |
thioesterase | - |
Escherichia coli |
thioesterase | - |
Haemophilus influenzae |
YciA | - |
Escherichia coli |
YciA | - |
Haemophilus influenzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
25 | - |
assay at | Haemophilus influenzae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
propionyl-S-n-acetylcysteamine | absorbance of 5-thio-2-nitrobenzoate formed by reaction of 5,5'-dithiobis(2-nitrobenzoic acid) is measured, Kcat is above 0.0001 | Haemophilus influenzae | |
0.0062 | - |
4-hydroxybenzoyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Escherichia coli | |
0.078 | - |
4-hydroxybenzoyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
0.082 | - |
n-butyryl-pantetheine phosphate | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Escherichia coli | |
0.1 | - |
beta-methylcrotonyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Escherichia coli | |
0.27 | - |
arachidoyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
0.54 | - |
beta-methylcrotonyl-CoA | absorbance of 5-thio-2-nitrobenzoate formed by reaction of 5,5'-dithiobis(2-nitrobenzoic acid) is measured | Haemophilus influenzae | |
0.55 | - |
acetyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Escherichia coli | |
0.77 | - |
stearoyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
0.8 | - |
n-butyrylpantethiene phosphate | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
0.92 | - |
crotonyl-CoA | absorbance of 5-thio-2-nitrobenzoate formed by reaction of 5,5'-dithiobis(2-nitrobenzoic acid) is measured | Haemophilus influenzae | |
1.1 | - |
methylmalonyl-CoA | absorbance of 5-thio-2-nitrobenzoate formed by reaction of 5,5'-dithiobis(2-nitrobenzoic acid) is measured | Haemophilus influenzae | |
1.3 | - |
acetyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
1.7 | - |
oleoyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
2 | - |
linoleoyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
2.6 | - |
tiglyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
2.7 | - |
palmitoyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
3.3 | - |
malonyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
3.7 | - |
palmitoyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Escherichia coli | |
4.6 | - |
n-octanoyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
5.7 | - |
palmitoleoyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
6.7 | - |
n-butyryl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Escherichia coli | |
6.9 | - |
myristoyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
8.3 | - |
DL-3-hydroxybutyryl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
8.9 | - |
DL-3-hydroxy-3-methylglutaryl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
9.2 | - |
lauroyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
9.4 | - |
phenylacetyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Escherichia coli | |
9.5 | - |
isobutyryl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Escherichia coli | |
9.6 | - |
lauroyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Escherichia coli | |
13 | - |
oleoyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Escherichia coli | |
13 | - |
n-decanoyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
15 | - |
hexanoyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
17 | - |
n-decanoyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Escherichia coli | |
22 | - |
n-butyryl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
25 | - |
propionyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
30 | - |
isovaleryl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
35 | - |
acetoacetyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
75 | - |
4-hydroxyphenylacetyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
100 | - |
glutaryl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
130 | - |
phenylacetyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
140 | - |
isobutyryl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
140 | - |
3-hydroxyphenylacetyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae | |
190 | - |
succinyl-CoA | pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay | Haemophilus influenzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
7.5 | - |
assay at | Haemophilus influenzae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | noncompetitive inhibition of YciA-catalyzed isobutyryl-CoA hydrolysis is observed which defined a Kii of 0.0047 mM (Vmax altered) and a Kis of 0.33 microM (Vmax/Km altered) | Haemophilus influenzae | |
0.0019 | - |
hexyl-CoA | - |
Haemophilus influenzae | |
0.0054 | - |
hexyl-CoA | - |
Escherichia coli | |
0.0058 | - |
4-hydroxyphenacyl-CoA | - |
Haemophilus influenzae |