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Literature summary for 3.1.2.20 extracted from

  • Wei, J.; Kang, H.W.; Cohen, D.E.
    Thioesterase superfamily member 2 (Them2)/acyl-CoA thioesterase 13 (Acot13): a homotetrameric hotdog fold thioesterase with selectivity for long-chain fatty acyl-CoAs (2009), Biochem. J., 421, 311-322.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.2.20

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged version expressed in Escherichia coli BL21(DE3) Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0073
-
 myristoyl-CoA at 25°C Mus musculus
0.01
-
 palmitoyl-CoA
-
 Mus musculus
0.0106
-
 myristoyl-CoA at 37°C, in the presence of StarD2 protein in a molar ratio StarD2/Them2 of 0.5 Mus musculus
0.0136
-
 myristoyl-CoA at 37°C, in the presence of StarD2 protein in a molar ratio StarD2/Them2 of 1.0 Mus musculus
0.0155
-
 myristoyl-CoA at 37°C Mus musculus
0.0159
-
 palmitoyl-CoA in the presence of StarD2 protein in a molar ratio StarD2/Them2 of 1.0 Mus musculus
0.018
-
 palmitoyl-CoA in the presence of StarD2 protein in a molar ratio StarD2/Them2 of 0.5 Mus musculus
0.0214
-
 myristoyl-CoA at 50°C Mus musculus
0.0228
-
 myristoyl-CoA at 37°C, in the presence of small unilamellar vesicles composed of phosphatidylcholine at 4 mM total phospholipid concentration Mus musculus
0.0274
-
 oleoyl-CoA
-
 Mus musculus
0.0275
-
 lauroyl-CoA
-
 Mus musculus
0.0472
-
 decanoyl-CoA
-
 Mus musculus
0.1382
-
 hexanoyl-CoA
-
 Mus musculus
0.1423
-
 malonyl-CoA
-
 Mus musculus
0.1628
-
 3-hydroxybutyryl-CoA
-
 Mus musculus
0.1916
-
 phenylacetyl-CoA
-
 Mus musculus
0.3057
-
 3-hydroxy-3-methylglutaryl-CoA
-
 Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information optimal range for ionic strength is 80-160 mM for both NaCl and KCl Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15000
-
 4 * 15000, gel filtration Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brown adipose tissue
-
 Mus musculus
-
heart
-
 Mus musculus
-
kidney
-
 Mus musculus
-
liver
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0069
-
 substrate 3-hydroxy-3-methylglutaryl-CoA Mus musculus
0.014
-
 substrate malonyl-CoA Mus musculus
0.0298
-
 substrate hexanoyl-CoA Mus musculus
0.0304
-
 substrate myristoyl-CoA, at 25°C Mus musculus
0.0322
-
 substrate myristoyl-CoA, at 37°C, in the presence of small unilamellar vesicles composed of phosphatidylcholine at 4 mM total phospholipid concentration Mus musculus
0.0377
-
 substrate lauroyl-CoA Mus musculus
0.0381
-
 substrate palmitoyl-CoA Mus musculus
0.0454
-
 substrate 3-hydroxybutyryl-CoA Mus musculus
0.0469
-
 substrate myristoyl-CoA, at 37°C Mus musculus
0.0543
-
 substrate decanoyl-CoA Mus musculus
0.0568
-
 substrate palmitoyl-CoA, in the presence of StarD2 protein in a molar ratio StarD2/Them2 of 0.5 Mus musculus
0.0604
-
 substrate oleoyl-CoA Mus musculus
0.0614
-
 substrate myristoyl-CoA, at 50°C Mus musculus
0.0652
-
 substrate palmitoyl-CoA, in the presence of StarD2 protein in a molar ratio StarD2/Them2 of 1.0 Mus musculus
0.0681
-
 substrate myristoyl-CoA, at 37°C, in the presence of StarD2 protein in a molar ratio StarD2/Them2 of 0.5 Mus musculus
0.0846
-
 substrate myristoyl-CoA, at 37°C, in the presence of StarD2 protein in a molar ratio StarD2/Them2 of 1.0 Mus musculus
0.1198
-
 substrate phenylacetyl-CoA Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxy-3-methylglutaryl-CoA + H2O
-
 Mus musculus CoA + 3-hydroxy-3-methylglutarate
-
 ?
3-hydroxybutyryl-CoA + H2O
-
 Mus musculus CoA + 3-hydroxybutyrate
-
 ?
decanoyl-CoA + H2O
-
 Mus musculus CoA + decanoate
-
 ?
hexanoyl-CoA + H2O
-
 Mus musculus CoA + hexanoate
-
 ?
lauroyl-CoA + H2O
-
 Mus musculus CoA + laurate
-
 ?
malonyl-CoA + H2O
-
 Mus musculus CoA + malonate
-
 ?
myristoyl-CoA + H2O
-
 Mus musculus CoA + myristate
-
 ?
oleoyl-CoA + H2O
-
 Mus musculus CoA + oleate
-
 ?
palmitoyl-CoA + H2O
-
 Mus musculus CoA + palmitate
-
 ?
phenylacetyl-CoA + H2O
-
 Mus musculus CoA + phenylacetate
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 15000, gel filtration Mus musculus

Synonyms

Synonyms Comment Organism
ACOT13
-
 Mus musculus
acyl-CoA thioesterase 13
-
 Mus musculus
Them2
-
 Mus musculus
thioesterase superfamily member 2
-
 Mus musculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 Tm 59.2°C (transition beginning at temperatures in excess of 50°C) Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information inactive with acetyl-CoA and 2-butenoyl-CoA Mus musculus
0.0066
-
 palmitoyl-CoA in the presence of StarD2 protein in a molar ratio StarD2/Them2 of 1.0 Mus musculus
0.007
-
 3-hydroxy-3-methylglutaryl-CoA
-
 Mus musculus
0.014
-
 malonyl-CoA
-
 Mus musculus
0.03
-
 hexanoyl-CoA
-
 Mus musculus
0.031
-
 myristoyl-CoA at 25°C Mus musculus
0.033
-
 myristoyl-CoA at 37°C, in the presence of small unilamellar vesicles composed of phosphatidylcholine at 4 mM total phospholipid concentration Mus musculus
0.038
-
 palmitoyl-CoA
-
 Mus musculus
0.038
-
 lauroyl-CoA
-
 Mus musculus
0.046
-
 3-hydroxybutyryl-CoA
-
 Mus musculus
0.047
-
 myristoyl-CoA at 37°C Mus musculus
0.055
-
 decanoyl-CoA
-
 Mus musculus
0.057
-
 palmitoyl-CoA in the presence of StarD2 protein in a molar ratio StarD2/Them2 of 0.5 Mus musculus
0.061
-
 oleoyl-CoA
-
 Mus musculus
0.062
-
 myristoyl-CoA at 50°C Mus musculus
0.069
-
 myristoyl-CoA at 37°C, in the presence of StarD2 protein in a molar ratio StarD2/Them2 of 0.5 Mus musculus
0.086
-
 myristoyl-CoA at 37°C, in the presence of StarD2 protein in a molar ratio StarD2/Them2 of 1.0 Mus musculus
0.12
-
 phenylacetyl-CoA
-
 Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 9
-
 Mus musculus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.023
-
 3-hydroxy-3-methylglutaryl-CoA
-
 Mus musculus
0.1
-
 malonyl-CoA
-
 Mus musculus
0.22
-
 hexanoyl-CoA
-
 Mus musculus
0.28
-
 3-hydroxybutyryl-CoA
-
 Mus musculus
0.63
-
 phenylacetyl-CoA
-
 Mus musculus
1.2
-
 decanoyl-CoA
-
 Mus musculus
1.4
-
 lauroyl-CoA
-
 Mus musculus
1.4
-
 myristoyl-CoA at 37°C, in the presence of small unilamellar vesicles composed of phosphatidylcholine at 4 mM total phospholipid concentration Mus musculus
2
-
 oleoyl-CoA
-
 Mus musculus
2.9
-
 myristoyl-CoA at 50°C Mus musculus
3
-
 palmitoyl-CoA
-
 Mus musculus
3
-
 palmitoyl-CoA in the presence of StarD2 protein in a molar ratio StarD2/Them2 of 0.5 Mus musculus
3.1
-
 myristoyl-CoA at 37°C Mus musculus
4
-
 palmitoyl-CoA in the presence of StarD2 protein in a molar ratio StarD2/Them2 of 1.0 Mus musculus
4.4
-
 myristoyl-CoA at 25°C Mus musculus
6
-
 myristoyl-CoA at 37°C, in the presence of StarD2 protein in a molar ratio StarD2/Them2 of 0.5 Mus musculus
6
-
 myristoyl-CoA at 37°C, in the presence of StarD2 protein in a molar ratio StarD2/Them2 of 1.0 Mus musculus