Cloned (Comment) | Organism |
---|---|
His-tagged version expressed in Escherichia coli BL21(DE3) | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D65A | active site residue | Homo sapiens |
D65E | active site residue | Homo sapiens |
D65N | active site residue | Homo sapiens |
H134A | reduction in catalytic efficiency | Homo sapiens |
H137A | conserved among vertebrate homologues | Homo sapiens |
H56A | active site residue | Homo sapiens |
K136A | relatively small perturbation in catalytic efficiency | Homo sapiens |
N50A | active site residue | Homo sapiens |
S83A | active site residue | Homo sapiens |
T69A | active site residue | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
desulfoCoA | - |
Homo sapiens | |
phenylacetylmethyl-CoA | - |
Homo sapiens | |
undecan-2-one-CoA | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0049 | - |
n-decanoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.005 | - |
myristoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.009 | - |
oleoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.009 | - |
palmitoleoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.009 | - |
myristoyl-CoA | wild-type protein, pH7.5, 37°C | Homo sapiens | |
0.0099 | - |
lauroyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.01 | - |
3,4-dihydroxyphenylacetyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.016 | - |
palmitoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.02 | - |
stearoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.02 | - |
Arachidonoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.025 | - |
3,5-dihydroxyphenylacetyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.026 | - |
3-hydroxyphenylacetyl-CoA | H56A mutant protein, pH7.5, 25°C | Homo sapiens | |
0.026 | - |
n-octanoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.032 | - |
3-hydroxybenzoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.049 | - |
4-hydroxyphenylacetyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.07 | - |
n-hexanoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.11 | - |
beta-hydroxybutyryl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.12 | - |
3-hydroxyphenylacetyl-CoA | K136A mutant protein, pH7.5, 25°C | Homo sapiens | |
0.12 | - |
beta-methylcrotonyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.13 | - |
4-chlorobenzoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.16 | - |
3-hydroxyphenylacetyl-CoA | T69A mutant protein, pH7.5, 25°C | Homo sapiens | |
0.18 | - |
crotonyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.19 | - |
n-propionyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.21 | - |
methylmalonyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.22 | - |
2-butenoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.23 | - |
3-hydroxyphenylacetyl-CoA | D65E mutant protein, pH7.5, 25°C | Homo sapiens | |
0.24 | - |
phenylacetyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.25 | - |
tiglyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.28 | - |
malonyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.29 | - |
acetyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.3 | - |
3-hydroxyphenylacetyl-CoA | N50A mutant protein, pH7.5, 25°C | Homo sapiens | |
0.31 | - |
3-hydroxyphenylacetyl-CoA | S83A mutant protein, pH7.5, 25°C | Homo sapiens | |
0.31 | - |
linoleoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.36 | - |
3-hydroxyphenylacetyl-CoA | D65N mutant protein, pH7.5, 25°C | Homo sapiens | |
0.41 | - |
3-hydroxyphenylacetyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.6 | - |
n-butyryl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.62 | - |
3-hydroxyphenylacetyl-CoA | H134A mutant protein, pH7.5, 25°C | Homo sapiens | |
0.67 | - |
glutaryl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.9 | - |
3-hydroxyphenylacetyl-CoA | H137A mutant protein, pH7.5, 25°C | Homo sapiens | |
2.9 | - |
3-hydroxy-3-methylglutaryl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9NPJ3 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-butenoyl-CoA + H2O | - |
Homo sapiens | CoA + 2-butenoate | - |
? | |
3,4-dihydroxyphenylacetyl-CoA + H2O | - |
Homo sapiens | CoA + 3,4-dihydroxyphenylacetate | - |
? | |
3,5-dihydroxyphenylacetyl-CoA + H2O | - |
Homo sapiens | CoA + 3,5-dihydroxyphenylacetate | - |
? | |
3-hydroxy-3-methylglutaryl-CoA + H2O | - |
Homo sapiens | CoA + 3-hydroxy-3-methylglutarate | - |
? | |
3-hydroxybenzoyl-CoA + H2O | - |
Homo sapiens | CoA + 3-hydroxybenzoate | - |
? | |
3-hydroxyphenylacetyl-CoA + H2O | - |
Homo sapiens | CoA + 3-hydroxyphenylacetate | - |
? | |
4-chlorobenzoyl-CoA + H2O | - |
Homo sapiens | CoA + 4-chlorobenzoate | - |
? | |
4-hydroxyphenylacetyl-CoA + H2O | - |
Homo sapiens | CoA + 4-hydroxyphenylacetate | - |
? | |
acetyl-CoA + H2O | - |
Homo sapiens | CoA + acetate | - |
? | |
arachidonoyl-CoA + H2O | - |
Homo sapiens | CoA + arachidonate | - |
? | |
beta-hydroxybutyryl-CoA + H2O | - |
Homo sapiens | CoA + beta-hydroxybutyrate | - |
? | |
beta-methylcrotonyl-CoA + H2O | - |
Homo sapiens | CoA + beta-methylcrotonate | - |
? | |
crotonyl-CoA + H2O | - |
Homo sapiens | CoA + crotonate | - |
? | |
glutaryl-CoA + H2O | - |
Homo sapiens | CoA + glutarate | - |
? | |
lauroyl-CoA + H2O | - |
Homo sapiens | CoA + laurate | - |
? | |
linoleoyl-CoA + H2O | - |
Homo sapiens | CoA + linoleate | - |
? | |
malonyl-CoA + H2O | - |
Homo sapiens | CoA + malonate | - |
? | |
methylmalonyl-CoA + H2O | - |
Homo sapiens | CoA + methylmalonate | - |
? | |
myristoyl-CoA + H2O | - |
Homo sapiens | CoA + myristate | - |
? | |
n-butyryl-CoA + H2O | - |
Homo sapiens | CoA + n-butyrate | - |
? | |
n-decanoyl-CoA + H2O | - |
Homo sapiens | CoA + n-decanoate | - |
? | |
n-hexanoyl-CoA + H2O | - |
Homo sapiens | CoA + n-hexanoate | - |
? | |
n-octanoyl-CoA + H2O | - |
Homo sapiens | CoA + n-octanoate | - |
? | |
n-propionyl-CoA + H2O | - |
Homo sapiens | CoA + n-propionate | - |
? | |
oleoyl-CoA + H2O | - |
Homo sapiens | CoA + oleate | - |
? | |
palmitoleoyl-CoA + H2O | - |
Homo sapiens | CoA + palmitoleate | - |
? | |
palmitoyl-CoA + H2O | - |
Homo sapiens | CoA + palmitate | - |
? | |
phenylacetyl-CoA + H2O | - |
Homo sapiens | CoA + phenylacetate | - |
? | |
stearoyl-CoA + H2O | - |
Homo sapiens | CoA + stearate | - |
? | |
tiglyl-CoA + H2O | - |
Homo sapiens | CoA + (2E)-2-methylbut-2-enoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | crystal structure | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
hTHEM2 | - |
Homo sapiens |
thioesterase 2 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | no activity observed with 4-hydroxybenzoyl-CoA, lauroyl-acyl carrier protein, myristoyl-acyl carrier protein, palmitoyl-acyl carrier protein, octanoyl-acyl carrier protein, oleoyl-acyl carrier protein and acylated peptide N-acetyl-Gly-Ala-Cys-S-palmitoyl, turnover number of mutant protein D65A below 0.00001 s-1 for 3-hydroxyphenylacetyl-CoA | Homo sapiens | |
0.0032 | - |
4-chlorobenzoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.0044 | - |
palmitoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.0047 | - |
n-octanoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.007 | - |
3-hydroxyphenylacetyl-CoA | N50A mutant protein, pH7.5, 25°C | Homo sapiens | |
0.0076 | - |
crotonyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.011 | - |
oleoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.011 | - |
stearoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.011 | - |
3-hydroxybenzoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.014 | - |
2-butenoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.016 | - |
n-decanoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.017 | - |
linoleoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.017 | - |
palmitoleoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.019 | - |
lauroyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.02 | - |
myristoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.02 | - |
tiglyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.022 | - |
Arachidonoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.022 | - |
n-hexanoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.023 | - |
beta-methylcrotonyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.036 | - |
n-propionyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.047 | - |
n-butyryl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.05 | - |
glutaryl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.054 | - |
acetyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.066 | - |
malonyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.07 | - |
myristoyl-CoA | wild-type protein, pH7.5, 37°C | Homo sapiens | |
0.079 | - |
3-hydroxy-3-methylglutaryl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.083 | - |
methylmalonyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.084 | - |
phenylacetyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.14 | - |
4-hydroxyphenylacetyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.15 | - |
3-hydroxyphenylacetyl-CoA | S83A mutant protein, pH7.5, 25°C | Homo sapiens | |
0.16 | - |
3-hydroxyphenylacetyl-CoA | D65E mutant protein, pH7.5, 25°C | Homo sapiens | |
0.16 | - |
3-hydroxyphenylacetyl-CoA | H134A mutant protein, pH7.5, 25°C | Homo sapiens | |
0.18 | - |
beta-hydroxybutyryl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.19 | - |
3,4-dihydroxyphenylacetyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.19 | - |
3,5-dihydroxyphenylacetyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.32 | - |
3-hydroxyphenylacetyl-CoA | K136A mutant protein, pH7.5, 25°C | Homo sapiens | |
0.36 | - |
3-hydroxyphenylacetyl-CoA | D65N mutant protein, pH7.5, 25°C | Homo sapiens | |
0.62 | - |
3-hydroxyphenylacetyl-CoA | H56A mutant protein, pH7.5, 25°C | Homo sapiens | |
0.66 | - |
3-hydroxyphenylacetyl-CoA | T69A mutant protein, pH7.5, 25°C | Homo sapiens | |
0.83 | - |
3-hydroxyphenylacetyl-CoA | H137A mutant protein, pH7.5, 25°C | Homo sapiens | |
1.4 | - |
3-hydroxyphenylacetyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
undecan-2-one-CoA | 0 mM NaCl | Homo sapiens | |
0.0033 | - |
undecan-2-one-CoA | 100 mM NaCl | Homo sapiens | |
0.009 | - |
desulfoCoA | - |
Homo sapiens | |
0.5 | - |
phenylacetylmethyl-CoA | - |
Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
3-hydroxyphenylacetyl-CoA | N50A mutant protein, pH7.5, 25°C | Homo sapiens | |
0.025 | - |
4-chlorobenzoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.027 | - |
3-hydroxy-3-methylglutaryl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.054 | - |
linoleoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.064 | - |
2-butenoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.078 | - |
n-butyryl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.082 | - |
tiglyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.17 | - |
3-hydroxyphenylacetyl-CoA | H134A mutant protein, pH7.5, 25°C | Homo sapiens | |
0.18 | - |
acetyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.18 | - |
n-octanoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.19 | - |
n-propionyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.19 | - |
beta-methylcrotonyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.24 | - |
malonyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.28 | - |
palmitoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.31 | - |
n-hexanoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.35 | - |
phenylacetyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.35 | - |
3-hydroxybenzoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.39 | - |
methylmalonyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.47 | - |
crotonyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.58 | - |
3-hydroxyphenylacetyl-CoA | S83A mutant protein, pH7.5, 25°C | Homo sapiens | |
0.67 | - |
3-hydroxyphenylacetyl-CoA | D65E mutant protein, pH7.5, 25°C | Homo sapiens | |
0.75 | - |
glutaryl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
0.92 | - |
3-hydroxyphenylacetyl-CoA | H137A mutant protein, pH7.5, 25°C | Homo sapiens | |
1 | - |
3-hydroxyphenylacetyl-CoA | D65N mutant protein, pH7.5, 25°C | Homo sapiens | |
1.1 | - |
Arachidonoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
1.3 | - |
oleoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
1.6 | - |
beta-hydroxybutyryl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
1.9 | - |
lauroyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
1.9 | - |
palmitoleoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
2.6 | - |
3-hydroxyphenylacetyl-CoA | K136A mutant protein, pH7.5, 25°C | Homo sapiens | |
2.9 | - |
4-hydroxyphenylacetyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
3.3 | - |
n-decanoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
4 | - |
myristoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
4.1 | - |
3-hydroxyphenylacetyl-CoA | T69A mutant protein, pH7.5, 25°C | Homo sapiens | |
5.5 | - |
stearoyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
7.7 | - |
myristoyl-CoA | wild-type protein, pH7.5, 37°C | Homo sapiens | |
7.9 | - |
3,5-dihydroxyphenylacetyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
18 | - |
3,4-dihydroxyphenylacetyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens | |
24 | - |
3-hydroxyphenylacetyl-CoA | H56A mutant protein, pH7.5, 25°C | Homo sapiens | |
35 | - |
3-hydroxyphenylacetyl-CoA | wild-type protein, pH7.5, 25°C | Homo sapiens |