Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression in Escherichia coli BL21(DE3) as His-tagged enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetoacetyl-CoA | competitive inhibition | Homo sapiens | |
acetyl-CoA | competitive inhibition | Homo sapiens | |
DL-3-hydroxybutyryl-CoA | competitive inhibition | Homo sapiens | |
DL-methylmalonyl-CoA | competitive inhibition | Homo sapiens | |
isobutyryl-CoA | competitive inhibition | Homo sapiens | |
malonyl-CoA | competitive inhibition | Homo sapiens | |
n-valeryl-CoA | competitive inhibition | Homo sapiens | |
propionyl-CoA | competitive inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
(S)-3-hydroxy-2-methylpropanoyl-CoA | recombinant enzyme, pH 8.0, 30°C | Homo sapiens | |
0.025 | - |
3-hydroxypropionyl-CoA | recombinant enzyme, pH 8.0, 30°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
gel filtration | Rattus norvegicus |
38000 | - |
1 * 39398, amino acid sequence calculation, 1 * 38000, recombinant enzyme, SDS-PAGE | Homo sapiens |
39398 | - |
1 * 39398, amino acid sequence calculation, 1 * 38000, recombinant enzyme, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-2-methylpropanoyl-CoA + H2O | Homo sapiens | - |
CoA + 3-hydroxy-2-methylpropanoate | - |
? | |
3-hydroxy-2-methylpropanoyl-CoA + H2O | Rattus norvegicus | substrate is an intermediate in the pathway of valine catabolism, which is unique in that it produces a radily diffusible monocarboxylic acid | 3-hydroxy-2-methylpropanoate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
from rat liver, 7200fold, to homogeneity | Rattus norvegicus |
recombinant enzyme from Escherichia coli, removal of His-tag with thrombin | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
heart | - |
Rattus norvegicus | - |
kidney | - |
Rattus norvegicus | - |
liver | - |
Homo sapiens | - |
liver | - |
Rattus norvegicus | - |
muscle | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
244 | - |
purified recombinant enzyme, substrate 3-hydroxypropionyl-CoA | Homo sapiens |
427 | - |
purified enzyme | Rattus norvegicus |
430 | - |
purified recombinant enzyme, substrate (S)-3-hydroxy-2-methylpropanoyl-CoA | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxy-2-methylpropanoyl-CoA + H2O | - |
Homo sapiens | CoA + (S)-3-hydroxy-2-methylpropanoate | - |
? | |
3-hydroxy-2-methylbutyryl-CoA + H2O | low activity | Homo sapiens | CoA + 3-hydroxy-2-methylbutyrate | - |
? | |
3-hydroxy-2-methylpropanoyl-CoA + H2O | - |
Homo sapiens | CoA + 3-hydroxy-2-methylpropanoate | - |
? | |
3-hydroxy-2-methylpropanoyl-CoA + H2O | - |
Rattus norvegicus | CoA + 3-hydroxy-2-methylpropanoate | - |
? | |
3-hydroxy-2-methylpropanoyl-CoA + H2O | substrate is an intermediate in the pathway of valine catabolism, which is unique in that it produces a radily diffusible monocarboxylic acid | Rattus norvegicus | 3-hydroxy-2-methylpropanoate + CoA | - |
? | |
3-hydroxypropanoyl-CoA + H2O | - |
Homo sapiens | CoA + 3-hydroxypropanoate | - |
? | |
DL-3-hydroxybutyryl-CoA + H2O | low activity | Homo sapiens | CoA + DL-3-hydroxybutyrate | - |
? | |
L-3-hydroxybutyryl-CoA + H2O | low activity | Homo sapiens | CoA + L-3-hydroxybutyrate | - |
? | |
additional information | substrate specificity, overview, acetoacetyl-CoA, DL-methylmalonyl-CoA, isobutyryl-CoA, malonyl-CoA, acetyl-CoA, propionyl-CoA, and n-valeryl-CoA are poor substrates | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 36000, SDS-PAGE | Rattus norvegicus |
monomer | 1 * 39398, amino acid sequence calculation, 1 * 38000, recombinant enzyme, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Homo sapiens |
8 | - |
assay at | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.1 | 10.3 | at least | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
acetoacetyl-CoA | recombinant enzyme, pH 8.0, 30°C | Homo sapiens | |
0.14 | - |
DL-3-hydroxybutyryl-CoA | recombinant enzyme, pH 8.0, 30°C | Homo sapiens | |
0.72 | - |
n-valeryl-CoA | recombinant enzyme, pH 8.0, 30°C | Homo sapiens | |
0.83 | - |
propionyl-CoA | recombinant enzyme, pH 8.0, 30°C | Homo sapiens | |
1.19 | - |
isobutyryl-CoA | recombinant enzyme, pH 8.0, 30°C | Homo sapiens | |
1.6 | - |
malonyl-CoA | recombinant enzyme, pH 8.0, 30°C | Homo sapiens | |
2.26 | - |
DL-methylmalonyl-CoA | recombinant enzyme, pH 8.0, 30°C | Homo sapiens | |
2.61 | - |
acetyl-CoA | recombinant enzyme, pH 8.0, 30°C | Homo sapiens |