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Literature summary for 3.1.2.6 extracted from

  • Scire, A.; Tanfani, F.; Saccucci, F.; Bertoli, E.; Principato, G.
    Specific interaction of cytosolic and mitochondrial glyoxalase II with acidic phospholipids in form of liposomes results in the inhibition of the cytosolic enzyme only (2000), Proteins, 41, 33-39.
    View publication on PubMed
Search for more literature for 3.1.2.6

Inhibitors

Inhibitors Comment Organism Structure
cardiolipin negatively charged phospholipid, inhibits the cytosolic isozyme noncompetitively, specific ionic and probably also hydrophobic interaction Homo sapiens
dioleoyl phosphatidic acid i.e. DOPA, negatively charged phospholipid, strongly inhibits the cytosolic isozyme noncompetitively, specific ionic and probably also hydrophobic interaction Homo sapiens
dipalmitoylphosphatidylserine negatively charged phospholipid, inhibits the cytosolic isozyme noncompetitively, specific ionic and probably also hydrophobic interaction Homo sapiens
additional information negatively charged phospholipids, like dioleoyl phosphatidic acid, cardiolipin, and phosphatidylserine, specifically interact with the enzyme, but do not inhibit the mitochondrial isozyme, no effect by neutral phospholipids Bos taurus
additional information no effect by neutral phospholipids, phospholipid binding studies Homo sapiens
phosphatidylserine negatively charged phospholipid, inhibits the cytosolic isozyme noncompetitively, specific ionic and probably also hydrophobic interaction Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics in presence of liposomes consisting of different phospholipids Homo sapiens
additional information
-
 additional information kinetics in presence of liposomes consisting of different phospholipids Bos taurus
0.152
-
 S-(2-hydroxyacyl)glutathione cytosolic isozyme, pH 7.2, 25°C, in presence of dioleoyl phosphatidic acid Homo sapiens
0.152
-
 S-(2-hydroxyacyl)glutathione mitochondrial isozyme, pH 7.2, 25°C Bos taurus
0.162
-
 S-(2-hydroxyacyl)glutathione mitochondrial isozyme, pH 7.2, 25°C, in presence of dioleoyl phosphatidic acid Bos taurus
0.169
-
 S-(2-hydroxyacyl)glutathione cytosolic isozyme, pH 7.2, 25°C, in presence of cardiolipin Homo sapiens
0.191
-
 S-(2-hydroxyacyl)glutathione cytosolic isozyme, pH 7.2, 25°C, in presence of dipalmitoylphosphatidylserine Homo sapiens
0.191
-
 S-(2-hydroxyacyl)glutathione mitochondrial isozyme, pH 7.2, 25°C, in presence of cardiolipin Bos taurus
0.201
-
 S-(2-hydroxyacyl)glutathione cytosolic isozyme, pH 7.2, 25°C Homo sapiens
0.255
-
 S-(2-hydroxyacyl)glutathione mitochondrial isozyme, pH 7.2, 25°C, in presence of phosphatidylserine Bos taurus
0.283
-
 S-(2-hydroxyacyl)glutathione mitochondrial isozyme, pH 7.2, 25°C, in presence of dipalmitoylphosphatidylserine Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol cytosolic isozyme Homo sapiens 5829
-
mitochondrion mitochondrial isozyme Bos taurus 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-(2-hydroxyacyl)glutathione + H2O Homo sapiens enzyme is part of the glyoxalase system and involved in detoxification of methylglyoxal glutathione + a 2-hydroxycarboxylate anion
-
 ?
S-(2-hydroxyacyl)glutathione + H2O Bos taurus enzyme is part of the glyoxalase system and involved in detoxification of methylglyoxal glutathione + a 2-hydroxycarboxylate anion
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 mitochondrial isozyme
-
Homo sapiens
-
 recombinant cytosolic isozyme expressed in Escherichia coli
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-
liver
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-(2-hydroxyacyl)glutathione + H2O
-
 Homo sapiens glutathione + a 2-hydroxycarboxylate anion
-
 ?
S-(2-hydroxyacyl)glutathione + H2O
-
 Bos taurus glutathione + a 2-hydroxycarboxylate anion
-
 ?
S-(2-hydroxyacyl)glutathione + H2O enzyme is part of the glyoxalase system and involved in detoxification of methylglyoxal Homo sapiens glutathione + a 2-hydroxycarboxylate anion
-
 ?
S-(2-hydroxyacyl)glutathione + H2O enzyme is part of the glyoxalase system and involved in detoxification of methylglyoxal Bos taurus glutathione + a 2-hydroxycarboxylate anion
-
 ?

Synonyms

Synonyms Comment Organism
acetoacetylglutathione hydrolase
-
 Homo sapiens
acetoacetylglutathione hydrolase
-
 Bos taurus
Germ cell specific protein
-
 Homo sapiens
Germ cell specific protein
-
 Bos taurus
GLO II
-
 Homo sapiens
GLO II
-
 Bos taurus
glyoxalase II
-
 Homo sapiens
glyoxalase II
-
 Bos taurus
hydrolase, acetoacetylglutathione
-
 Homo sapiens
hydrolase, acetoacetylglutathione
-
 Bos taurus
hydrolase, hydroxyacylglutathione
-
 Homo sapiens
hydrolase, hydroxyacylglutathione
-
 Bos taurus
Round spermatid protein RSP29
-
 Homo sapiens
Round spermatid protein RSP29
-
 Bos taurus
S-2-hydroxylacylglutathione hydrolase
-
 Homo sapiens
S-2-hydroxylacylglutathione hydrolase
-
 Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens
25
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Homo sapiens
7.2
-
 assay at Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0002
-
 dioleoyl phosphatidic acid cytosolic isozyme, pH 7.2, 25°C Homo sapiens
0.0022
-
 dipalmitoylphosphatidylserine cytosolic isozyme, pH 7.2, 25°C Homo sapiens
0.006
-
 cardiolipin cytosolic isozyme, pH 7.2, 25°C Homo sapiens
0.055
-
 phosphatidylserine cytosolic isozyme, pH 7.2, 25°C Homo sapiens