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Literature summary for 3.1.2.6 extracted from
- Glyoxalase system in yeasts: structure, function, and physiology (2011), Semin. Cell Dev. Biol., 22, 278-284.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0166 | - |
S-D-mandeloylglutathione | pH not specified in the publication, temperature not specified in the publication | Saccharomyces cerevisiae |  |
| 0.0365 | - |
S-D-mandeloylglutathione | pH not specified in the publication, temperature not specified in the publication | Saccharomyces cerevisiae | |
| 0.0722 | - |
S-D-lactoylglutathione | pH not specified in the publication, temperature not specified in the publication | Saccharomyces cerevisiae | |
| 0.112 | - |
S-D-lactoylglutathione | pH not specified in the publication, temperature not specified in the publication | Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Saccharomyces cerevisiae | 5737 | - |
| mitochondrial matrix | - |
Saccharomyces cerevisiae | 5759 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31306 | - |
x * 31306 | Saccharomyces cerevisiae |
| 32325 | - |
x * 32325 | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
| Saccharomyces cerevisiae | Q12320 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-D-lactoylglutathione + H2O | - |
Saccharomyces cerevisiae | D-lactate + glutathione | - |
? | |
| S-D-lactoylglutathione + H2O | - |
Saccharomyces cerevisiae | D-lactic acid + glutathione | - |
? | |
| S-D-mandeloylglutathione + H2O | - |
Saccharomyces cerevisiae | ? + glutathione | - |
? | |
| S-D-mandeloylglutathione + H2O | - |
Saccharomyces cerevisiae | D-mandelate + glutathione | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 31306 | Saccharomyces cerevisiae |
| ? | x * 32325 | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Glo2 | - |
Saccharomyces cerevisiae |
| Glo4 | - |
Saccharomyces cerevisiae |
| glyoxylase II | - |
Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 25.8 | - |
S-D-mandeloylglutathione | pH not specified in the publication, temperature not specified in the publication | Saccharomyces cerevisiae | |
| 35.1 | - |
S-D-mandeloylglutathione | pH not specified in the publication, temperature not specified in the publication | Saccharomyces cerevisiae | |
| 723 | - |
S-D-lactoylglutathione | pH not specified in the publication, temperature not specified in the publication | Saccharomyces cerevisiae | |
| 979 | - |
S-D-lactoylglutathione | pH not specified in the publication, temperature not specified in the publication | Saccharomyces cerevisiae | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Saccharomyces cerevisiae | expressed only in glycerol medium | additional information |
| Saccharomyces cerevisiae | Expression occurs both in glucose and in glycerol media | additional information |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 710 | - |
S-D-mandeloylglutathione | pH not specified in the publication, temperature not specified in the publication | Saccharomyces cerevisiae | |
| 2200 | - |
S-D-mandeloylglutathione | pH not specified in the publication, temperature not specified in the publication | Saccharomyces cerevisiae | |
| 8700 | - |
S-D-lactoylglutathione | pH not specified in the publication, temperature not specified in the publication | Saccharomyces cerevisiae | |
| 10000 | - |
S-D-lactoylglutathione | pH not specified in the publication, temperature not specified in the publication | Saccharomyces cerevisiae | |
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