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Literature summary for 3.1.2.6 extracted from
- A glutathione responsive rice glyoxalase II, OsGLYII-2, functions in salinity adaptation by maintaining better photosynthesis efficiency and anti-oxidant pool (2014), Plant J., 80, 93-105.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as a His-tagged fusion protein | Oryza sativa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-lactate | - |
Oryza sativa |  |
| EDTA | - |
Oryza sativa | |
| glutathione | - |
Oryza sativa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.254 | - |
S-Lactoylglutathione | pH and temperature not specified in the publication, Vmax: 1 mmol/min/mg | Oryza sativa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Oryza sativa | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | contains a binuclear Zn/Fe centre in its active site | Oryza sativa | |
| Zn2+ | contains a binuclear Zn/Fe centre in its active site | Oryza sativa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28560 | - |
calculated from cDNA | Oryza sativa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-NTA chromatography | Oryza sativa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| anther | - |
Oryza sativa | - |
| callus | - |
Oryza sativa | - |
| endosperm | - |
Oryza sativa | - |
| inflorescence | - |
Oryza sativa | - |
| leaf | - |
Oryza sativa | - |
| panicle | - |
Oryza sativa | - |
| plant embryo | - |
Oryza sativa | - |
| plant ovary | - |
Oryza sativa | - |
| root | - |
Oryza sativa | - |
| seedling | - |
Oryza sativa | - |
| shoot | - |
Oryza sativa | - |
| stigma | - |
Oryza sativa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-lactoylglutathione + H2O | - |
Oryza sativa | GSH + D-lactate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Gly II | - |
Oryza sativa |
| glyoxalase II | - |
Oryza sativa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 508 | - |
S-Lactoylglutathione | pH and temperature not specified in the publication | Oryza sativa | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Oryza sativa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | - |
Oryza sativa |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Oryza sativa | slight downregulation during the early reproductive stage | down |
| Oryza sativa | slight upregulation during the late vegetative phase | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | ectopic expression of OsGLYII-2 in Escherichia coli and tobacco provides improved tolerance against salinity and dicarbonyl stress indicating towards its role in abiotic stress tolerance | Oryza sativa |
| malfunction | OsGLYII-2 is able to functionally complement a yeast GLY II mutant | Oryza sativa |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2000 | - |
S-Lactoylglutathione | pH and temperature not specified in the publication | Oryza sativa | |
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