Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Oryza sativa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-lactate | - |
Oryza sativa | |
EDTA | - |
Oryza sativa | |
glutathione | - |
Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.254 | - |
S-Lactoylglutathione | pH and temperature not specified in the publication, Vmax: 1 mmol/min/mg | Oryza sativa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Oryza sativa | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | contains a binuclear Zn/Fe centre in its active site | Oryza sativa | |
Zn2+ | contains a binuclear Zn/Fe centre in its active site | Oryza sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28560 | - |
calculated from cDNA | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Oryza sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
anther | - |
Oryza sativa | - |
callus | - |
Oryza sativa | - |
endosperm | - |
Oryza sativa | - |
inflorescence | - |
Oryza sativa | - |
leaf | - |
Oryza sativa | - |
panicle | - |
Oryza sativa | - |
plant embryo | - |
Oryza sativa | - |
plant ovary | - |
Oryza sativa | - |
root | - |
Oryza sativa | - |
seedling | - |
Oryza sativa | - |
shoot | - |
Oryza sativa | - |
stigma | - |
Oryza sativa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-lactoylglutathione + H2O | - |
Oryza sativa | GSH + D-lactate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Gly II | - |
Oryza sativa |
glyoxalase II | - |
Oryza sativa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
508 | - |
S-Lactoylglutathione | pH and temperature not specified in the publication | Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Oryza sativa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | - |
Oryza sativa |
Organism | Comment | Expression |
---|---|---|
Oryza sativa | slight downregulation during the early reproductive stage | down |
Oryza sativa | slight upregulation during the late vegetative phase | up |
General Information | Comment | Organism |
---|---|---|
malfunction | ectopic expression of OsGLYII-2 in Escherichia coli and tobacco provides improved tolerance against salinity and dicarbonyl stress indicating towards its role in abiotic stress tolerance | Oryza sativa |
malfunction | OsGLYII-2 is able to functionally complement a yeast GLY II mutant | Oryza sativa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2000 | - |
S-Lactoylglutathione | pH and temperature not specified in the publication | Oryza sativa |