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Literature summary for 3.1.21.3 extracted from

  • Endlich, B.; Linn, S.
    Type I restriction enzymes (1981), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 14, 137-156.
No PubMed abstract available
Search for more literature for 3.1.21.3

Activating Compound

Activating Compound Comment Organism Structure
S-adenosyl-L-methionine required Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 alpha2,beta2,gamma1, 2 * 135000 + 2 * 62000 + 1 * 52000, EcoK, SDS-PAGE Escherichia coli
55000
-
 alpha2,beta4,gamma2, 2 * 135000 + 4 * 60000 + 2 * 55000, EcoB, SDS-PAGE Escherichia coli
60000
-
 alpha2,beta4,gamma2, 2 * 135000 + 4 * 60000 + 2 * 55000, EcoB, SDS-PAGE Escherichia coli
62000
-
 alpha2,beta2,gamma1, 2 * 135000 + 2 * 62000 + 1 * 52000, EcoK, SDS-PAGE Escherichia coli
135000
-
 alpha2,beta2,gamma1, 2 * 135000 + 2 * 62000 + 1 * 52000, EcoK, SDS-PAGE Escherichia coli
135000
-
 alpha2,beta4,gamma2, 2 * 135000 + 4 * 60000 + 2 * 55000, EcoB, SDS-PAGE Escherichia coli
400000
-
 EcoK, sucrose density gradient sedimentation Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Salmonella enterica subsp. enterica serovar Typhimurium enzyme restricts the exchange of genetic material between bacteria of different strains or species ?
-
 ?
additional information Escherichia coli enzyme restricts the exchange of genetic material between bacteria of different strains or species ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 enzyme EcoR
-
Escherichia coli
-
 enzyme EcoK
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
 enzyme SA and SB
-

Purification (Commentary)

Purification (Comment) Organism
enzyme EcoB Escherichia coli
enzyme EcoK Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
duplex DNA + ATP
-
 Salmonella enterica subsp. enterica serovar Typhimurium double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate
-
 ?
duplex DNA + ATP the site of restriction cleavage is random, occuring between 1 and 5 kb from the recognition site. The modification methylase acts directly at the recognition sequence Escherichia coli double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate
-
 ?
additional information enzyme restricts the exchange of genetic material between bacteria of different strains or species Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
additional information enzyme restricts the exchange of genetic material between bacteria of different strains or species Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
octamer alpha2,beta4,gamma2, 2 * 135000 + 4 * 60000 + 2 * 55000, EcoB, SDS-PAGE Escherichia coli
pentamer alpha2,beta2,gamma1, 2 * 135000 + 2 * 62000 + 1 * 52000, EcoK, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
EcoK
-
 Escherichia coli
EcoR
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
167
-
 ATP at 37°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 nuclease reaction Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP required Escherichia coli
ATP is degraded during reaction Escherichia coli