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Literature summary for 3.1.21.3 extracted from

  • Jindrova, E.; Schmid-Nuoffer, S.; Hamburger, F.; Janscak, P.; Bickle, T.A.
    On the DNA cleavage mechanism of type I restriction enzymes (2005), Nucleic Acids Res., 33, 1760-1766.
    View publication on PubMedView publication on EuropePMC

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
recombinant
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
duplex DNA + ATP EcoAI preferentially generates 3'-overhangs of 2-3 nt. Displays some preference for the formation of 5'-overhangs of a length of 6-7 and 3-5 nt, respectively. type I restriction enzymes require two restriction subunits to introduce DNA double-stran breaks, each providing one catalytic center for phosphodiester bond hydrolysis Escherichia coli double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate
-
?
duplex DNA + ATP EcoKI displays some preference for the formation of 5'-overhangs of a length of 6-7 and 3-5 nt, respectively. Type I restriction enzymes require two restriction subunits to introduce DNA double-stran breaks, each providing one catalytic center for phosphodiester bond hydrolysis Escherichia coli double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate
-
?
duplex DNA + ATP EcoR124I displays some preference for the formation of 5'-overhangs of a length of 6-7 and 3-5 nt, respectively. Type I restriction enzymes require two restriction subunits to introduce DNA double-stran breaks, each providing one catalytic center for phosphodiester bond hydrolysis Escherichia coli double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate
-
?

Synonyms

Synonyms Comment Organism
EcoAI
-
Escherichia coli
EcoKI
-
Escherichia coli
EcoR124I
-
Escherichia coli