Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
recombinant | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
duplex DNA + ATP | EcoAI preferentially generates 3'-overhangs of 2-3 nt. Displays some preference for the formation of 5'-overhangs of a length of 6-7 and 3-5 nt, respectively. type I restriction enzymes require two restriction subunits to introduce DNA double-stran breaks, each providing one catalytic center for phosphodiester bond hydrolysis | Escherichia coli | double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate | - |
? | |
duplex DNA + ATP | EcoKI displays some preference for the formation of 5'-overhangs of a length of 6-7 and 3-5 nt, respectively. Type I restriction enzymes require two restriction subunits to introduce DNA double-stran breaks, each providing one catalytic center for phosphodiester bond hydrolysis | Escherichia coli | double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate | - |
? | |
duplex DNA + ATP | EcoR124I displays some preference for the formation of 5'-overhangs of a length of 6-7 and 3-5 nt, respectively. Type I restriction enzymes require two restriction subunits to introduce DNA double-stran breaks, each providing one catalytic center for phosphodiester bond hydrolysis | Escherichia coli | double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EcoAI | - |
Escherichia coli |
EcoKI | - |
Escherichia coli |
EcoR124I | - |
Escherichia coli |